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2B ~ >F-16 | [> =-@ Y¥/0 why = ston,
i/13)5 Raped. “= [3: surge Bl ied

ee
-

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Al bf $it+
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2 (se- 161471607) x Yo +e +>
W677]. ‘ a eth - &
. (G07 6
BA dew ( 922 Wn clits ) Uy P12 — 73° P77.
7 3916/1607 Y/o tH+ +7
& 4 13677 he
4 to? y/o oO
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lo “161 + 1607 ¥/o — +,
HH be2 YrO- “pT 5 gfe nw [oT be Te pees a Cc

A. Uent Bhee~ +1 bace. Colt rit YO -b0 lae -cgtruie
btn wW/6o7, hisrtolke £. Powenrng V:YI P47. > V9 Vo tact us Thosly
No lact Abel am Hakone t} , 1 (reget IH pop ithe < 10°).

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6 58-181 x W18N7 4+ tt

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& “ x wW 101) qd ++

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Trahsmission of F+ 899

1/15/52

1. Washed cells. Mix 58bhb1 and W-1607 in saline. Incubate 3 - 9 PM. a) Streak out
on EMB Lac; b) Inoc. Penassay + sm lOug/ml. ¢:A” All XX x Y-10 unless indicated

2. Grow 58-161 + W1321 in Penassay. a&b as above. (a showed background of Lact and lambd:
plaques. le CHurfors. » Maou bess Hanceeec Tid Urbina F+ hao).

3. Transmission from lambda-sensitive to smumcthinw, W-1655 + W~-1607 in Pennassay. Then a&b

4. ~ ’ to sensitive.W-1655 + W-1321....

5. Pe ‘to W~1177. W-588 + W-1177 a&b. (b showed rare + pap. & culture was pure.)

In all cases, Lac- colonies from a) were pooled to make fresh inoculum.
Cultures were restreaked to control success of resiolation of F- component. Growth

from sm-Penassay in b) was used directly, in each case with only barely detectable
Lac+ residuum. .

6. Transfer via lambda? 58-161 streaked out on W-1321 on EMB Lac sm. Plaques restreaked.
Individual colonies picked and tested for lysoggnicity. #1 (out of ca 25) was lysogenic

reo” rechecked. Singah verified lysogenic colony retained for test of
F: (X y~10

7. Single colonies of W-1816 from stock culture (itself re&solated) streaked on EMN Gal(-).
o = stock,

Tests (x Y-10 exc. 5, x W-1607)

a b c(= retest pooled Lac- colonies from test streaks of b)
1 - 7 cols. 47 wtto?tonf. —
27%* +44 ++ tht
34 +44 +++ +o
4 as v4 +++ tor
' £& -
5 +44 C- ae grit , nee 3 eee ws slide Y= WI8I7
(wn77F+)
6 ~_ OM doco ut pauend Fr. U2.
7 Litt+ 2+++ 3+++ A+++ O+++ W-1607 -. F+ of W-1816 is therefore heritable at

least 50 cell generations.

8. Crude supernatant of 58-161. 4dd sm 1l0ug/ml. Inoc. W-1607, ineubate 1-7 PM. Limited
overall growth: Reinoculate loopful to Penassay am for cross inoculum, and streak
out on EMB Lac. (ca! / tec+)

9. Whote culture 58-161 inoc. ca 1:20 sm Penassay. Add W-1607 as above....
< 109% Leet

10. Dense eell suspension 58-161 aerated, as above.... < APs bac
Crosses of sm-selected W-1607 treated component.

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