626 y A UBTY UGS” : ahah flees hee tMARt 14 exrtibsrauts : pnt, ne Ghes) B w7 MT Lac x I20S or EMS, $lach) 2F Mel+- qe MGs-n4l No 4 628 Sele thn; 4 dung ercefont Retants . £30 ‘ t I S ql : Vi+ WILT + 130 w] 8 Arche ( il = go Vl lace 34. WG urne Mal Kgl - Hie — $300 lar —6¢ Nal a) I Kyhe nets Ke B) / 2 Kode r al — 1 KyR- rte + Mad t- 7] / Xgl fi barca | ¥ Me Nak Tan | * ayer f= f= f= | = ( 2 ’ - + — — ai2 - - + _ a] a& - +. a - 5 1 — aad = + + | 22 =e _ — - 7]/ & | + | + nn 2 iy of — — @ d + + + + ~ ~> dan | gtr te is Jes eg toft [ace [8 Yule [Mite b/s | feA- Tl oe Masi yyer | 3/4 | Malt hy ae fa MH -48 - ee eee ai; 1B,; 22 op Oe 6304 (Of -6 f/f ty, SPY Ye Ww P ~ ~ » + UAL fs aw Sab F=8 SVE Us bp - > r \ -~, S al \3 hee — -4.. 51/-3 tot; Ww erorrlo. 63) AA 17,1991 _. __ _ _ A lias 6 0s e | i22 . 2 ( Dee ues pom eopmeeyh to [arly 9. Al 4 Flo) 3IEMS. depres BR 3 1 ail oh Wee, c. a! yo O cats. D &" lo" [? tacy talon Oo EMS | )0 exlorter Ej s 7 st Ss T/p L EMS leew E . feels : 2 bar Fale Make fies [Fe | bee) Mat 9 Lac—Relt Halt. LU = _ + x + — G&G — Movciusrrtan> t + — + * ; = o| — | — | ¥ |x 7| 7 _ t * €| + + - + * 4) = _ + * loo t+—- | +- | +- el 4 +-|- |e) x eR Fal ee | * 3 + = > RE Sor ~ ; 6 + ~ is 1) + —~ _+r PassAato $ ~~) 7 ~ e — > —— Racer poorly EMS be kebba 24, /tys &3}a_ Lhe nm, = ~~, ¥ ° Lace! Mag— Mal- bac —| Male [Balt lect | Mel bral — Lac Nalt+ bul lee. - Halt ae rf lee— | /talt rT Kal + ts Nal bE ] Tal + ®e © Tan 4 pr lag l Ht. 63Y t 794. _ 58-16) x WE7T. Gpuak | fk p, TB) petals HM HM whl tree ,eageaad Dh 18 sake - | CrinttaY PIP ere : ol, Att O 194,479, 209 leo: IB2-,2736 Ail Oh i> Pay) 4"? 170, 20%, 207,2)3 19S 24 25° | 2°? R281 193 3| 3° | 47 446 , 1% (see!) 390,407 286 y| ye g to 26S ilo 240 1a! 194 si 52% | oe [az 404 142 2621 j New coli strains—~ crosses 63 October 26, 1949. . First prepare inocula overnight. Then mix.1 ml inceula, grow together 36 h. Wash and plate on (0), A. 1117” COB, 135 «6, 1197 :« 2D. 1222 EB, 1222+466 F. 1222 + 477 @ 122241117 ‘6;,0,0 : -170,0, QO, 1? 30,0 9,051 : 6 94935253 1,0 Qaceninine H. 1222+ 1197 I, 1222 + 1135. 000000 0000000 W-1222 is Val~ ArgGlut—- Mal-Gal-Lact Other stocks are Lac-. Yields (per plate, 3 dags) See above a Note. prototrophe on E (W406 (K22f x We1222. Streak out on EMB Lac to purify and test: wm _“Lac Gal Mal Sucr T5- WehioG tHe W-1222 + - - +s R 7 ee ~ —- + R pr lL + ~ +*- + Ss _¥ 3 F - + + § * Y +. _ - + p 6 fF - -~ F RP 7 * ~~ — + &f f @ + - - tp R ey 4 er ee f 10 a 3 x co MO Oe OR. P Mor 7 — tf Ok p 3 we + + + & “ _o+ - — + R p | -Oeteber 25 "BY 1949, -- Regtest diploids on synthetic medium. - Both these cultures, therefore, are segrgating for Az r/s. _ Dominance of Az™/® in diploids 635 _ W 1217 x W-677 on Lac EMS. Good yield. Pick 100 Lac+ and streak out on " Lac EMB for Lac# vy. 14 Lacy found. [All were lambda+ /518]) © Reisolate to EMS Lac. 635=1 and 635-9 streaked out, and Lac- and + segregants isolated. Test on NA + Az. : (NA controls all +) 635 -1 Oo | =8 5 Lac- lr 4s _ 2 3s $ Lact 5 r Os - se le 4 Lacy all r (segregants!) all r.. ~~" Note totals: — Lac- 3:7. - ~ Lact @:1 indicating linkage of Az to Lac, with frequent - -- Mal duplex prototrophs 636 — Oct. 25:1949 =O" 5@=161 x 677. --EMS Mal. Look for Mal+/- sectored prototrophs. 7. found. Stweak out on EMS Mal to seprate components. from severak hundred. htt ; ! + = ty - = -_- s $ 2 + 7 _- = + - + — $ Ss S + = - + —- 7 fe Ss G + + _- — ++ +* RR 5S + _~ _ = _—-~ -- S$ 5 Cc Fr + + +7- r- $s Ss 7 + - + - += + - 5 S$ Het incrosses 637 ; Nov. 7» 1949. - k, 1235 x 1239 B. 1243 x 1239 C. 1238 x 1236 “D. 1238 x 1242 A) heavy - background EMS Lac By Crosses by NZ 20 small +/pl B) about 200 large +/gl -indisting from bekg C) Heavy background 200+ several -, many intermediates p). Heavy-bekg. no = many large+ ___ 200 from each to EMB Lac to look for Lacy a EMS fac akeo— Aft bac v — BLS bev Nal — Mak+ Mao rt cnc hes fn Keo (F, 5) EP 2 oY he i hetek #3lac- Lact 5 Bee 3 & Tecan 4 . TT. rt © L oT 6 (DL TY T te TT gv L. rs 4 L T . 10 u a Sepmanchiss toad Aicees 22. 639 Veventa 10,1999, kept b27 A Yrary i239 alle, eae - Imelt | pgpensin, pe 6 JL¥axnr3q EMS fae Ae, Ta —P / c 1235 ¢ a . | Oo 9% Entols Toabd ! ——_ - Heck pis wth recta, ead bo heseg! Ake |KO Lace fens r life ne z ?2 | Restahn, ESI hae; S7ab.. _& 4I2-39- Mme tal. — Uovenece 138, 1149 ee : ad HoIR , W2 aensb-wiS 4) smnnng ate. oven a eal ff he frome Wek-72 I ef 639 An bh Ane. HO 2x jd "ea, foo bf) re" wWiR IT - 29%. hg a bYo Uprernbe. 13, 1499. _ _ PID Anau V5. 8 IZ Weah W217 cud) e177. Hen sets ad no: oy ra) a. 3) 7 "oe Ik NgSoy Meter . , 4 " on Mr pi fr KiA pee WINB 673 He. “4, PYF | ego Eum wen. culls pba. bak sara BI ond 87 ade mtg, OM. A) ea [ote donk nt peter Whe, fe (erdiphays) & eo 12% fay. FmL/Fdrnek ee Ygpites i4 K-12 Mutant Kerr (Sars fore) bY fas]. . Wk alge 6 pci Acheter vin Yr beth Ce pete mane + houhadt. Glruce ftLH a, rls). EnBlac; Yurb.€. Wr Lex Lipreeyy dnt “fio ap : Tih EM Slee. - he puonrd Ne Lek: Jd grees fey gon on 0) 1 Lb not qo T/8) . Sun deo. K-/2R x WIS/D. ESS lav 40 f oR | te, Vi es to A.wibe x 1429 8 BYTE x Ma 6 pe f | toh tandonn Ow NSS 2 a op eT 24* aatl, § Sec ~ —€ ¥ ny G a of Aicke f 6 oF Fo « Mtbe ok tiga? fob Suer + py Sicen~ waned) Qe satin, WISE war b wr F pee ai OTT peg (ten Sen) care on deen, Lac, TS, Tb: 1h ~ all Sees baer TO" 5% | Oo aA " Ef Fy Dt. 23 duc~ hace 2 THEE” Vo senel ld toeombekois dD Seep bare FE 7. Apeured. SO MMi hhe sere! - ts vane lg ae ade " W "y Drrumane 2f the SR Ane. 6Ys- A 3/14. a wR WIT. Jo pla». nly a palotngh» what 3 a bay. Teed mgepanks om SK. | all (al — | tl 12 vik. thir are V,> ea ae a Aenalict ila 9 bow by ous 8, lac- 2, > axe 8%; Mie a) Fick nA AB fon EYSMA a, “Engle and EMG lee tsh Jeo. UV affts no Aeplof YS iN ve - | - 4Mal ~ cols foie H2I> 10 wrtfas. “uv teh) » Hee. 2 HH+e 3 YMH— lo” '/303. av. Ob mele 2,3 Me - a 4. 4 NK Loe al pe fatty valores. a 696 - ce ne. () sale]. : to” and eo? mL H2)> > (form ES bac) aaion en’ Oe EMG He. Cape tone & WY, 3 scene. a re uy. + v . fo Oo I Jo 4 oO 1 os f ( 3 — / ff TOTAL 3 q 62 _— °/, — jo~ 3 a |o a “ oe § § 8 12. _ 3 6&© FF. i f 44 H io? = 3 / |. | § o 0 2 ToTAL 13 33 2.3 wk Fe ise ye oO 2 wr Qt _3 ye 70 22 +S 6 385 43 | | 2 25 7 ry oe A 23 4 a7. So 92 147 Aq y fx exaament 7-0 Bc { l 7 10 _ 2 oO a 13 - 3 er y o 8668s il . 2 at ¥3z _2. Le Lu 2) | 34 62 us | 33. [23 | 74 201 SH J 4 16 6 bs fo Bec. ge + — : *s 8 . Ys 28 BY 4 z 16 5 2 22- 16 33 . H /0 | 37 4 “ ‘g 7 H ‘Ss 31 > 3 3 3 19 | 37 6468 sos v Tote. Ao 65 55k tay , 3232209 73.8. 6 Irradiated 3 secs. — | ea. IO surv. 1g 33 3B 7h Bh 4h FL 32 125 170 327 1038 52 WIZsT y Le sy fedheotasn EHSlac aor ay Wp lacy. 1/s/yi | | Wyse xesT glen EMB Laz. ft “p" Cc Wye? x Wag (upsfra) oo 650 NEW YORK STATE COLLEGE OF AGRICULTURE ‘CORNELL UNIVERSITY Widesday Deay 4 of. lec jet 5 s£° } 4 Alre , 10, th 12463 cette H 926 - /, a phd hae eet: lprief) "pow a eg Gu cutting - ,. Aru: . babe Toh Gt qey CH but n Cat oe bet appsh 1 ot EOE Cet te. pe tha Coney, 10 + if wel ote “ Eel lore un tal Cpe tey cuuke ths yeh i Pwbarad, a Cpe f~=pe “SS CKerves. LN ih otis cei ele, EE IE ras vn toe uae emt ot a Aa «, ee lpg, fr Ary 2, Alen, Brae | TE er Cleef te 1d- ney sf x-270 col 12. “04, er eo )33 < nee - | 270 so. - ' Chon sand, than 272) goth % fpr 0 ao cap haul 703 3 delet pons | 2704 Aromas om bone ned wo her. (054903 colrvay le Aporrtd 105 33 by : a hoe ta Zz saul avert 4 bey the ampere. QT ten, ee 2 ce rae ea fel pecs eo re ran ree ett He Ae ath NEW YORK STATE COLLEGE OF AGRICULTURE CORNELL UNIVERSITY AL hertir 1 ot abha ak op Cari yg, thet. a0 eet tell cal be, | eget tic £ torky ond eduy eth cc Ani evrce ling ET fwd calle a i wate Be B a ki 12~ 94 eknval 6 te, 199 whew sc eZ 9 newt ee Lin ; JZ 3b ced 13-1863 poy wo 15% 1s, otbt , a,pk wpa Ln, 103 op afrre inhale ~33 a Ne Tart + Prchtiny, y. : 1-3 ¢ 4-74 v0 Colgre,s, COBMETT fo4TAEBeLLA u JosK 21N.LE COLTECE OF VOBICKN LIK: