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| {

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isolation of biochenica - masents

4

wy se

Lutents 1 were “isolated from Stock cultures of (ye IG 10) and

 

 

\cultures were grown in comrlete

medium without either aeration or subsequent irradiation. ijashed

cells were incubated in minimal medium, to

whieh various amounts of

penicillin (100, 150 and 200 units rer ml regnpectively) were added.

By using the replicdéd plating technicue, mutants were isolated

in 4 experiments.

Experiment Stock
le WG 10
Le ir 10
Be WG 15
4, Wo 10

lveineless
(mutant 7)

 

Biochemical mutants

 

- histidineless
Leoleucine-valineless
- methionineless

- lvsineless

U @ tb
'

- iseleucine-velineléss
- lysineless

- histidineless
lysinelese

Ga fay fe

Rs
'

}
i)
1

S52 all prolineless

FLX - diauxetronh
Lysineless and unknown factoz

Subsequent testing indicated thet the following were stable

mutentse Others were discarded as repeated isolates of the came

mutation or for other reasons.

\ A, - histidineless ee a es

~ en
+

“bY +

isoleucine-velineless
~ De. - lysineless

ij E - isoleucine-valineless

NJ

- lysineless
H - lysineiess... o
oy a
: \ le vate
24.- prolinélese [WS °* )

PLY - diauxetreph so? 5
lysineless 4 unknown factor.
ate
ht

WG Mutants and Crosses

A description of all WG mutants made will be found on ¢

seperate sheet. The first number given the mtant is the

one undgr which the mutant can be found in my notebook;

the second number given is the W number, The chart indicates
which mutants were obtained in the same experiment and the
number in parent@thes indicates the experiment number in

my notebook. All mutants were selected by the penicillin
method. Sp indicates that the mutants selected had arisen
spontaneously; U.V. indicates that mutants were induced by
means of ultraviolet light. Four seperate attempBs to put

a marker other than histidineless or prolineless on W1895_ ?
failed.

The following crosses were made between WG strains:

 

Strain WGs Crossed Mutants used Golonies/Plate
WGLL ii x 13 see under WGL3
WG12 12x 12 1865 x 1868 ca 10
12 x K12 1868 x 58-161 ca 50
1868 x 1177 ca 30
12 x 3 1365 x 148 0, 2
1868 x 1448 ea 5
12x44 1863 x 1445 1, 2
12x13 see under WG13
WG13 13 x 13 1901 x 1902 1 to 6
13 x 12 1902 x 1868 ca 200
13 x Kil2 1902 x S11 6 to 10
13 x 11 1902 x 1883 2, 0, 0
1902 x 1915 1, 6, 0
1902 x 1834 2, 0, 3
1882 x 1353 1, 0, 0
ise2 x 1915 0, 0, 0
1332 x 1884 0; 0, 0

 
 

 

 

 

 

 

 

   

 

 

 

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