ca LL. . 27 lactarar 20 ram -alep tf 6 SY Wetull el rkophte’ oni | ' ttt empl | 04H 1. Shoes, tas" isp rg e tte 3 So totale, en's. - pq. | | | Be | : 3 reise isi \ i : 132 | rae : ! SYo | 179 530 | 27S 4S . _ : 128 : 128 p/ - tat Gso} vere * a ow a a 33 i 26% G) oN PQ PONG HE pe a 69 ro e | : i | i ; ; | ! : | 4 : : | t : 7 : nb @ ow Lb | YP | BI S30. | | | : : | 79 |beOn- YR © 275, 468 = a | : | : i ( 1ohe»,) BRON Be YP — 7-a “rove | => ®~ & ~ Ss OUD | | 034 Ove 022 OYw, 036 OS O ut 3 oa eyo 053 133 03] O14 te oy 670 068 072 $1 eh | 660 183 . CBO Of 7 | | 4 i } i Of | (2S ORD | 673 2383 03 i t pee 08 . rn a 193- Wyuf 131 90 —2// AAE _ [oe 139 wd pe? 9D IS? IBS 147 WS NON: GalNiagd HALAWITV IW "OD N3SSZ15IG0 3Nagna - : Yadvd HdVHO NIOZLFIIG W- OVE ON 630 % A Z QP A \ Lego N5 |e LES All eat 770) 24 ft Do bs 1 2*° () r” (re) 2” (a0) Vas d2” 30197 (vo) 3” ¢sa) 2” %o) floso |29f logo O76 J /¢6 io bY 133 Yo yovg (0740 |\090 O74 | por /06 104 jz] 433 jIy3 O47 1293 |693 O97 | Wo | 16? No jr! \32— | IYO don 094 1095 09E | O92 | O92 | 096 | 097 | OFF | 142 a7 |e63 1066 O66 668 O76 | 080 | 090 | OF¢ /67 I © -| S q Ky 1G 28 42. | 4q s ° 3 3 s 13 17 27 33 44 3-F +8 HY 30 3 30 30 YA 37 33 eclim 3 - i y so eat @ s+ (43-17) Hg = 4 | 3° (45. ye tus) 432 (14 ya? lisp | B Tp 198 (229° Juss) | 2 po 1 /9/ |2?! | 2bo 3 ly? | 140 250 y¥ /O2 | ld 3] S HQ | Jro 6 S 8 $y 104 127 160 YALaAwWiTiw wwoSCA NI GaLNIN j J BQ Na9zi3id SN39N3 MadVd Hav NIOZLIIG W- Ore ON 7 . _ — fe (ENGI I 0 cgay . _ IP wk ralorrnrectt fbr. link 2. [3m ell, (1-100 N/joo WhP. — raljwwo aun. b) gon [gant alm era. s)eemy Divtg , ae | yoo | pt | 92 | 82 yp | Le [@\\ 80 0&3 10490 94 HO [80 , tee | 260 ne 2/670 OB MO J2¥ | 139 | 2S | 225 12k 0 BayoS NR 1129 | 146 | 187 YO 257 1327 bioso joSS | 663 | 479 | 0&0 | 109 HS | (20 a 2/560 1669 199° loa |}06 [26 | 12S | 131 3|67@ (090 (106 (32) [128 | 146 | 149 | 144 © LO as So ts |}y4o | Is> L/o |} | 30 30 27 24 30 A ! BS 14/6 | Bo 24 36 4 33 $7. 97 IS 7 “ 3i ts 2% 123 | 25 3! q4 108 1178 ¥Q Op Les. t O10. Re btreact™ OF0 far 1-3 ! 67 2_ frag Lelie’ bens o = blag 6 os lac | Mak } blir / Sue by Hel. (bh) oS fac ol Nel Sul pln OS Sue De Pompey I2© 370 120 {Oo 147 WP 176 IS& oll O73 Os2-~ OS 182 Fo 080 O67 ey). 089 COD we ge KA. 370 (Leer IP Care . sofjo, [ee pei Peecqens WAS -— 817, PARS \ | t+ ~~ BP (* S De Cong. OL O20 37, _A B ./ 284 ec os O60 4?) p ./ O0%o o7¢ GG. ‘A 008 —0/0— Hi!7 B tl. O50 06Y tc 10s OS9 690 dD .t ee oY7 uf 13 aur Ft / ¥2 Tat *. eg) cet r-k ‘ Usrnerd / 07? AOt A» ven 1,28 A Fi. hes feder a Se ut fa ‘bin Fin, " oO 35+ panne Ls E Jo. 944 370 ra SO . hose, yt lad rammprler ; De de ‘ hiner 4 207 803 qb b ye Yb0 346 c 23 5 20 397 0 2 = =9§ 30 — 408 Ne aclirnlion f goles preatinacl chs Her mew colle vi unt !! Coe gtncs Moth K-12, Paull 9 . Wee 28fr474. oy di Dongs. RK 196) 269 A. ERIE) 304 O ~ 00> oY7 Rrareney 72 PAN UR (Af) Zoy 260 ZK (ine) 190 277 46 (Gp) pol 97 SR (med 154 S20 Lb (Haye. Io 670 oO 003 Wat gest alr uals of Passat Galactosidase: cells from synthetic med. 12/8/49 647 Harvest K-12 from 30 hr. unshaken cultures in Davis minimal medium (new). Cone. 90:10. Intact cells Dy Lac OAL Mal 059 Glu 053 Extr. (Bz ttment) Lac Mal Giu .O1 ~003 2 053 4 108 ~004 0.2 ml per tube ea D sae 064 057 103 057 097 004 7 608 > ~(d:{0.9) + 6d§ + Dong Acer H73 003 ov | 098 go} — 0238 AA. AA. 1o70 " steal,” I96o/ Y, | aly 1? | “e rneed K -/2 ~— 48h, 5 Come 90:10 t4 ha. bla SE ¢ feces meena) hat cel : Olek tebe ; Qin. eruicl cake (Begins Sherco) RA u/s ee 7 e ‘of Jo! ifs fre / i NY 0 See 0o8Y OF; 0.4 wh. «127 oh “ an Bak Me es . G6 off an wlia/yy . Fagrace frbeo 7 (0) tiuiwlhre + euppleimacls wo reatcaled) Lo 7 — | 2. felon | 1% + AN cl te “3. hep one 5% + ey w Y¥. Y.Cu. .] % H+ -—M Seperate ote ? 4S. he. bayh toetary «(0 = _ hn H(. " 5% +L — AW wre tS mA. Fhe v 9. Uits | —- 4 mM vy 1. 4VAL+ YA. | _ re he — WAR. = A Coser, + oF Syos “1a. ADE ag teas “YM AY ee oe “is AS - A + “7 me > Aon) veh. patnconenttity U, ewbluce Urb OH A hprecr. a a ot njr0 b pattich fron nk Yhov 7