Arse om paloty . hes eg ees ax rt 2.19 P LX ++ 1,392 Ss [Me ta. Be | Ia he o| x - fo 2 Youd! H 5 PY & ( Phe 8.4 2 & Aclonre Rt 7,20 7 (AS) a. hl ge 3.10 y (Alestis? ) sf) + 2.67 7 helo C. _ 7. _ g. 7. ~ 0. — Placer ++ 9.98 — Xt Bly core +4 6.24 — Wile ?? Larter. ~ 0.1% Prada Copy 0 thor + I abt 3. Cre dour, af Ua saturation, Boy Seah /6 | E amare ha Wer} bclre | jour uk ly. Ae Bch No yg St int. ch hu. py 0 heres. ‘ed a) Ko acolt FR / " MBI caret N 5. pow ech I Paik Coan. Aeon “fe 4 A pat 4 A Ae } im dO Sook. ra Frachin, | ve (15 AS) 4 Fa bw VU . v \ Vive fuck /, bee qt bes a utyct PEK (so%AS) / \ %y AS. Fratin, 2 [15 HA) Joe AS. U LTUe pph . ~ hey la A ae Pye. Eaton S . / | Hasan : luk OS nl 2. Fratton | (hy sake) Ab operant 3 F ‘A Ch sat.) Qo f. FS Off se.) hea cg. FY (gah) Lear pS Reside fle, ASeal. V, optelerend Nesng wth 5h lulose Wyle 27" )6(b >/20 L30- 2.4] AL afl. wR BT 3; Ht 8TH NS th de po. IDSA 9.4/0 8/ R 13.Yo- 45,70 2,30 frie uit ancforoy R[20,. 48,70 - 16.70. hivlaltef. bYecer, o. ptnhy peur (bet Mtibld anes the “anrrtable ceo dbere Hu VfAS areol fhe A AS pastes. brtinin te ayting thee prt ‘ap ot WAS. bet ys f ad portion’ . ~ aie on Pts >?) o? st 4 si + 7! wk I, hhelonr Se ( Aare Duan. Cc. b) .4 La fe ie 7 _ S. > ‘ _. E ode S11 Santis co tall hasty. — 2,93 PAS ¢ ‘. = + 9,90 Nh. cha 0 Muda bt “op: a Ake —~ cmditone te » haat ‘o T Ly tare ling + B aindt mee 16 fo Bn soph. ¢ AS DY ter racine sretamed)- CN) Seaperecatiod — G6 Cc 2) dRemTebh rides offer esterprurenens n't O vil red Cy 0 Cc >) Non- xdomundebl perschice . ~ Cy 0- Id wh reuples (in Binal Sep covngonse unt wrtrkewoeslone. >, (243.2) Yo° be ts ZL a Papasln of lactase (Babel, 2. Y62 - Duma K- JL mM j2Q. V deg ae { 0 Facrerg fo ae stg necator, . Afar LIL. awead is, lap es (Ur fan,). Fashions '. 315. packs ~ hth oon HD, Stack ftetrcr , wry vw, phen Cmernedelg ye of 97° HA 24 Peaks 2. Yq paste . fl /000-N fectie sie ek, hime + 2d fuel acetnn 2 Kea gL , ay sceiceitr, cpt PIS. 4g. (ancl deg ') pork. Seungernl Woy. ees Pee ml Hob baer (eat ah w 16! b) : wk serpin ein, ( seiny ; YWo°). ee ard 107M K Mindy, Akh 11.56 AS (hrs) goa fudiradet on iyo. A ’ seperated B. Tol . Tod seme d tac I62-YA Cue Ue Vovbbe fo vey be “— ; fag nearny | a a V6 2a P28. haf bb. hrbetanak Cadkdls for in C4 Os tabee wap astra th fel decently fico d) 120 ul Alt chen . - tolulic, eathaly tar | YT elle rae nk sennple. — Worle with the othr 140 wl. a a AS. CLT ” 80 cae 4 oa Lf oo wndin. rc ye rot Cost wechgaele —2 IGA. 2. p fr ond) — (Redracebet mv 50 ml.) B. Aeten bin , Ol mk sonpleo oy loo mf tance ) Qom. 37° A . (Mable ay pats! Laden elle Liladaitd? | [A bens, nae tbeben’ J 7. Art. Repeat acme re Olwk. we M|i0 Na peasy weal) No frcbody bavtere prepuce Mah 24 tf | Joke, ha 11 ns W2e20. + Macro, Ub laa s lackore | (A) (6). Mele, 37° 214. (Mlk ankfem ). Gthed a3 Mong len bolle A bf Coll S34. hpet rom ©. [Beye AP nemart) AL. 8 aud an 100 wh. tall ut Vink. lure BL. Pq. gut am ok Sip lteter stale broffr 1h bh. Theor nrerhs eatin vod 1% potas . Clluat fl 16. Stree \p31 mi afc rm sheondennny fal, wet rcay it 2. aol | Lkceay bed . Hei ) yak —- whey UI Mi ml te sortdig sth / 2/0 Agetore. ) 30 Ke Lin . 3 7 ° wth, GN a tegen aed A oO + de ~ deg the certhin . , J e fee y 0. - ald bly ful tee shan, bl 7 tr. he fo nar le f . ~y BR? 1-0 + 0.1 _ 46> jo - 4636. t 62 th ty bon VG “iS Mbrasclot ppt ho. [aeeperalcce mecherdea Lox hrtatele af YI? J/iAr - Xa: 250 pbeliace tolore's Record Ww — 340 Tad a YE her v8 + 2S plB , xr00 3490 Seon fetal Sbiepae to vas # pct, al ¥ 36. Aly trae ++ t+ Dbseornie + + Holbrae t he Leche ++ - At 17 ths utah wv prreler b W-lo§ , brcb es Aachen etek, ming he aunt con ST fhare Fhe ghccryy mare fp 6, 1470. Mrabere . Joo paler x 270 = 30, [eo Vo toGhA wuterile Yd” /. Jd. IS. lt 17. T parent Mane. errands W- 340 Mo. WwW -d70 a1 OWA hth am 237° LT? o yor as 37° LIT" | al Lae VP of Cobbs Ahaverted fom l00nk Ana 37 [bt tho. = g 37=A ¥Y¥S°B (lls fron IP bee = I. (SOunk ue Lil Ho). Tet af 37 +t af >> of “f We 3S AM. pa X. ST ea YS i frac + +t one 2A | Sha. +e++ ne ol tes “ H+ tA ine * tH A jlo _ 2D flaw —_ YP | bee — _ Af war At ns Sinales . Ix vy, 8-10. 172 Lactase preduction; K-12, lactose synthetic mediun apr. 9, 1948. Inoc. $x 50 ml each. K-12 cultures into 10 1. bottles (2) of synthetic medium (v. supra) with 1.5% lactose USP. serate at 37° A9-Al0. Gollect in sharples. GT prawn dannoe A paste ln Jom? po ay ~ valine + nl pleene = POAID. Cahn ey pe ISO CC foray’ Sere Wow, oie oe ve oridy | eth) LX 45 AS. ph. | wm a ft tal ut taf ot clo iPro ened + + fhe tpt Artavrertel. The cup t Urelein oan na wert unl $00 n8 tah Be ey: AS atled. Te pli ell fe Se oA) Presid wath [emus (2, deasalet pf uc Sdaltto (GB). Whales GALL 4 ylbur aigaunh ) AO. 0,00 0.0] -©.0f oB. 124 7.34 10S oC. 1.42 44 YL Cro 1.38 l, 34 c/80 41 Pate #180. fy Ho actenk | /724& ) chbon f adap hibon ba ems aed Aagprecsts yy Pred v2, 1948 Gok Jule co rake b 1) ce. Cbs Ine Fro VP - ov VP -lchra | el | nih, 56 oathnn 5S mk Cony. Ch_ardats Jimnk Phrplat. (Aedfe (Wi0 ¥ lea, * les asatictime t (reg hadhny capac” +t (ng apa fern L. frre: = +++ ttt — tee 2. OL. tH + + $F i t Hee 3. Y. He +H - + aa 4 V+lL the | tte _ é pet. 5” Asp. 7~ g Mommnuchstegd? — YY + ™ oe Hofo . ~ : +++ ~ “ - 7. Arps Holle, He 5 tt — e are + arowuttoland th Oe / La bf i 7 . r i 2 hp 4, 1948 27:9 Z:Bo }/30 [ —_— ee ttt 3 ot: f —, yx, — G —— 7 — 8 _ gy 4. — 3:¢7O 3:30 + Ff + ttt tk 4+ tt SF 2_ Calls oon Yor (05 Uf) mL Wriear- Fe Junk cebl, wr foul. Sark tebe oternd ! link Sy /urk Y qo Lott —tldS ot I? DOK MM, dren dn 770 WH? : The (ecgprsitinr murtan 186 uj) - 340 and W- oF2. May 3, 1948. Add i drop iwcula to BCP-fermentation bnoth, at indicated temperature: W-340 glucose lactose maltose vane. gluconic 30° th tt — +b + it — $y 45° a ~ _. ~ - wen W-382 be , -_ 305 tt te tae — +h ° ay qt — - - ~ Hee oO v L a“ 45 — ~~ — - ++ free SPD. Feet Renda 8 BAN: 15h. Tire ore batt, temipere tect put hurls | W- 7 it nf Ah dank Fier itn adie fapte pelle and buctad ad bacfewe, /94 Pruett I3+ \- / uneeefars, or recy) Foufe It anf l— on aval feet ae syle ote [evanesetiou nufercls . 134 Vay 4 WE. Us. | dang vcorcule fo fost ye beth eliza 7 vical prrvtilion bith ACP ao avlkald 6 SP 1G 1 4+ +4 +4 oe Het fit te tE W-108 _ — — ++ - — — +f W->/0 +44 He Ht H+ _ — — tee W- D8. dee +++ ee 44 — — ~ + bin b PY. [Weiler 4 56-1 rn ren those ( (F-aabs 99S = 154. GAL ~39b,. Hl nsadhings ahueerl . Le. GAY= 6db. [oO Critical temperature: Temperature mutants. [70 May 5, 1948. W-340 and W-~382 inoculated inte BCP broth tubes at indicated temperatures: 30° Plus on glucose, lactose and maltose in 12 hours. an@ galactose 32° Ditte. Inecula from gma bréth .2 ml 33-34° Bitte. 5P 5. Inoculate W340, W-382, 58-161, W-108 as above. Tees. pevaltee Jhcele, a 9A6 16h. glu lac mal gal , ! f a — eee THE hoot Stay, BS a geh a, Thee 282 — ~ +++ — a $44 llc LOPE LE: { for lear 108 —_ a weer ~— . t+ t+- , ( i ake pees }- 4 54). Ny: Wa 58-161 met tt op )) ‘ a te ‘ — if "At 36% W382 2a bac r Bly - W327 WZST: adaptation on maltose LP May 6, 1948. Harvest cells of w-2b7 from overnight cultures of YP-broth. 50 ml. / 3 ml suspensions. A)- maltose 1% B)- gluconate 1% To lml 5% substrate, add 1 ml cells and 1 ml. .01 M Phosphate buffer plus BCP indicator. Incubate at 36°. Set up 11:15 A6. A glucose maltose gluconic A. — +. | — “> hee — = uv + B — — it v . ttt + - = To 1 ml. B cells add lee glticonate and .5 ml 1% triphenyl-tetrazolium hydrochloride. rttey dey td dry) Smee > Cphteyenl Shady od 4 A (Sate: ot 30) 401. AO febersucre ERE L a, ‘, tk. se) 3 RO. a yf 3:30 6PM, — - )724. ef a6 _ | 6 / “ss J dew W2FL 4 yr ¥2 bath. te « " f9~ coed gtictore. Seb “7 ud ft 1 P94 nm, Jy Jove. behecrgue ae Cea W BP | _ - "0 FW H+ te seal tt Wty ~~ * 1130 _ eee, TT Ranpoertin tant? — fhe, rxraes 19> bure. WedPL, We3¥0 und Be SE-16l inte PCP febes af 33° ¢¥o° ar ncuraked . 6Pé. lot Rin G47: 1Sb. 33° 0 acnwre Nawelel Eructe Sebvlof Nasrnare Nand Fuutere Sabihl tft ~+ vam t++e — “ — —~/% -4 — ew 4+ “ +h 7 tet ~ ~ fe < —_— Y _ / —_ ” ae tee ee te a yee We pee’ pep / He + + “4, af ~ eye uf. te Taf . hula Ager ~-Acaberiori X- abaphtrn slipfttcn. NY ‘dah 1918 Va cueet K- Nn Po LO a) ase (ret | : b) gala hres jogs AMT) | Dat "4 Hee anes oe ° . oes foil —— tubers ote Aeceped a Heo : — OF + . | - +H “ 8 . | | } +t Fees He | - ye tad- 1:30 lot | (2N 2A catece\ bee 100 [Adaphotin; ni prrtnce fret Mealy a ret x golectose | Cotun,'s EE £ - eathnnerst aud d- grlactess adepled cols | | she Lied) y W~382 195 May 7, 1948. Prepare 8 ml cell suspensions from 50 ml. YP broth cuktures (YZ-sugar) Cells: A: no sugar, B-glucose C- galactose D- lactose. Substrates: 1 glucose, 2-galactose 3- lactose. or at 40° xiutx After harvesting, icubate celhy without substrate or bufferd at 33-34° for two hours. Then (1:30 P 7) add 1 ml 5% sugar and buffer-BCP L frog 40 A B =. gts ren: $1 ge BR KH Ht ED ee - ¢ 3 ce t. ow! + =_— wi. # mop Mae. ~ en W-340 Exactly as above. Cells: A-glucose, B-galactose, C~lactose Substrates a& above. Le ait re «é Os . ee ef fos roles Av Rey By GO coed wb Ven F4e) oT bee eR ay) oS een Meal we me o : oo | ee a a i ya get Qhan - HA dg _ ov ae a 8g bY we hay _ _ 2 - = ‘ Af... oa we Cel Blaccrgpyrare aolaritive oF 89) bb os predicced daving ralectort adapter 0) 2PM. (r0 “3Onmunes ), eae 4 fen, a Bye Peunernaiy 1 9/95 19 Tectl for shebrbh af Yo" W382. pu) d¥0 ool Muccore Yolesto backrre fort Aehat antl Purest — Yadecbers re eR Lockear. bee eR ee at Bt? 0 ecg peace crs Aha i eal oP a abvstirce 5 bd ain gelaslore anal lator eclagild oll es Altes | @) Macey names <2 ahaproot af 37° (3 bation 2s pine teble at Yo". i Lenape tage fon WO are) W962 sides Hhaaaist crpaatle enndebis I [dar eubates pork ohbebh ?f Pelt dado liek tacts 1 196. ahrwneee f dubdale of “0° Way 8, 1948. Grow Y-10 and W-382 in 50 ml. batches YZ-sugar broth at 34°. A. Glucose (2 flasks each) B. Lactose (2 each) €. Gluconic ( 1 each). Dispense 1 ml. volumes to tubes with 1 ml indicator buffer (with and without azide)a/ Yo At stated times add 1 ml. substrate and record time required to ferment. Cells: A,B,C. Substrate: a,b Azide t+. — ; . Aat Aa- Abr Ab- Bat Ba- Bhr -Bb- * _ j o -. “of \ > Tame suboh fled): oO Ts HE) Hy Wher Pty 1,20 TIS 2 ar nf Ht yy 2 do ty Heic 15 thy a tru be tys Hy, tas oo ‘ wag ae é ey , + y 10 60 tr FO 7d tay FD tetas Tras FO tt {20 {le ve he: ee theo fey tiie FEN IMG / tT Go. +44 ~ Toy. try “of » ~ +E CO Fish ss” - - tty FI4, 30 tye Hye. Hs +42 23D a rth og WOrhy - L 30 py vs tyr the Fotyr OF tur tye tyr © > jbo se) . +t +1270 . hl 69 “ys WT tr %o ta 12 F277 ros oo Re 160 ribo [2s [yo +rspo- /a- / f 1 . Z fie —- 4 we \/ bee Pe | to > lo: dS an is Ss 11; 6 ‘ ye > way Go: tS 120 : mes ha hat for NS o fo +4 oe 7° 4 / : i fat Aa - Abt Ab - ‘Bat Pa - 3S o WS Is 30 30 176 | Bhr Bb ~ YS 30 . \ 30 30 IS ds 30 45 3o 110 60 vo 8 go do 30 Bo sh Ne YO 1l0 >Ie bo 30 FYSLJ26)30 > a *° ¢éo 30 YI -4 3O 60 30 YY NE 2e 10 <0 Yf-122) SS —_> Nall conti? on fol 7 W~-d8Q qlee liucrr yaar at 34 °/ Une an calle ff 292. Adole desoudt puvtut ther aerlbd, ean tice te fetnan cneedeldiy, 982 ducceroruana, Peppes gic pd “2 “a unrrhtlte co 6 ghaceysiane ty hee LE ie, forse Nenpeenlice drreerloce bee ~ /47 Vey! 5, /9 SG. hoc Lac -M2xe- OP fevmioblen tales engply fone sf. Honk. #5 SP) é 30° 375° 4a°- WY, —~ ~ — - - -— — _ — W-NO -— = - te ttt 4 te 4 ttt W905 = + ++ coo HR Os +h Y-/0. +h ye og Ht th ste HOH Ott Ot 0. V4. he = IVherr @) 7 PI6 =2Fh. & GA17 = 34h. Coty bechrar wo 60rnL V2 Lae froth ells beecled) i, fork 4,0. hceeasuy 7 ‘ofecl ‘ala An “(| Leal dnffr HTS at 37° upc t4/yem. /OMyus se tier wi . Cosel Le : cere. “lay A> Ylo Az A 20 »> 650 St an bd Fh Wt 13 ca SO Jf 065" 09 .o8 oT] a hc ied 074 ea 10 ,o] 009 008 orf op ,036 ,0/0 ,025 < i001 007% 00" ,022 07 — 1023 <¢ & 650 LO Correction, = rv; n”. 650° . At ip Hae maanl,, Yor FAA 10 ms feats 5 bed Penge = 10 ey Bolinns shoved ee ey: Ab Caucryp aarnd 2- A eeeell. Ayo eso Nye DV ese x ce A —o1" Vos 334 HSE LS’ “| | alle Hf 64 h- :7F0 523 ONP, CT. Mx coat bff alse. k= Y20. SgG000 1O70~ | C. D. t / 1065 1Y40- ADL L 7s 4 27 p- 4 ,272. Sad .YO>o~ BIS G 6 8 18- 8 isi 10 614 10 6149 4: 420 A> SOM eo .20 ,O7 (7 1 oy [Omne rm WPS my shan SHdS vare CPE SS] 6 Or + cre Ayah evees nie thls SA 4k Auger tuber’ tlk ofS ffs Yrs traf. lL. Loe ke OPS 2. Lew OPE 3, Ma 4. Mak " gs. 00F " *Oiaiaes Hea tacgs at 37°. leo Mate. Dino. %M%so Note. aulAhta neblgen gtierea. 4. 1032 oO te tad 3 080 o/79 | % O62 ° > 1290 ofS