fprveeg > L9G.

Mow ally

Y/Om

SD in -

YP oq Tooled m giant. ee

Reounml

A af ceatl te dae

BG | I byt

c sary fo +++

dD. facta /%. +4
- Toe. te 5 nl anh
er

 

SO}

te GUE DE ti
i Shes ll boy ths Conernbalin,
Metabolism of W-106. 17S,

feb, 11, 1948.

Harvest 2 batches (a.B) of #-108 grown in 50 ml. 1% YP-gluconate broth overnight.
Test sample for genetic purity.

A. (10 Ad) Cone. to 12 ml. Use 1 ml cells per tube, with $ ml. 10% sugar and
phaesphate-indicator. (No azide!)

gna gnafgl = gl gal galfgl Bu-gal. Bugal/glu aa
li aM +++ +44 ++ _ - — - —~
ee ft - -~ nis ~ -

Pst

yey
aa: 4 ml. cells 4 1 ml. gal. uN ml. phosphate-indicator for adaptation,to falactose.

B. ll aM «as above. Cone to 10 ml. 1 ml. cells/tube

gba glu gna gna-glu gal gal gnagal gigal Megal.
Ways - - + + oo ~ x _ —
JLN = - +++ +++ — - +++ - ~
igo — ~ - en “ - —

C Cob), Aq. Wart, aud fertas:
30/4. Dna Bh, al X gh Kal A hiek.

4:90 _ —

 

——
UW) -/08 C - Sorte Lhaghon hex
4 /3, (9VR

/ $0 0 both, rw. CIO ler f, 28
enh as /0 fo frat fe avbutr sty. 2 tol HO.
ot <p VIYTSAYY dnc. 37°
lose fh, lis lak Oallee Gur bral foo. pa Ma yf Xult bl, FPoa

~ hE
Wid0 0 + + ~ + _ ce - + pe
[2:30 + +E + 44 + ++ ~ + )
MPM +
M + th + tee RHE tHe ++ -
SPM te tte ttf og fe tt Steh tt HH
/! Peo /. o a lt ~ . fo (f a

Me deaf Halt He bead lars fekel

1 |
1
{

+t t++ +t4-
Gf -)

at

Halal nd oo Alacra pt —
accumulation of monoses by mutants -327,254 156

March 15-16, 1948.

Grow Y-10 & W~254 into YP 1% Lactose, 2x50 ml. each.
Y-10 & W+327 into YP 1% ialtose, do.

Harvest each, and concentrate in 10 ml volumes in sugar .5%, phosphate M/100.
At same time set up no-cells blanks, Lu:
Incubate at 37° 9A-1P 16. Add 4 ml. Barfoed's reagent to clarify. Boil
supernatants 10 mins. Cool, add 1 drop dil. aerokol CT to wet Guy0 ppt, and

sediment and wash in HO. Take up sediment in acid ferric solution and titrate

against .0200 N permanganate,

1. Y-10 Lae 0.10 There is therefore an almosty¥equimolar accumulation
2.Y-10 Mal 0.10 254
3.327 ©6Mat 0.30 of monose by $R¥, but none by 327 on lactose and maltose
4.254 Lac hee

e—-~ «Glu 9.40 respectively.
6.---- Mal 0.98 | @x@R
7.---—-Lac 0.28

The blanks contain 5 mg. sugar each. Note approximately 10% recovery pf maltose,

but negligible recovery of lactose.

seep remainddeys off suspensions 1 und 4 for further characterization of

the accumulated material.
Tetee Ik Exp. curpnens + nan
. ty Send ln, ppt a baba

dist CrP ppt, warda © hearduton ec. Famed tf

Feta os.

|, hicoan + Pheapbate
2. Neltears Phos phake

3. Yio tCbiie

Yo W3rT

5 Plow ial

v/ 00 MH Oy.

22,60 ~ 12.71
2355- 22.60
2.3.55 —- | Line. We lon

23-69 — 23.91.

2351 — < (Arog.
Fnartoashn of Gt’ lackiz jéo
i

 

Mah, VO -12, 1998.

X la Log fo ape pee ~s¥

GY Valk wt unrdh, MCR .7Y. waned
al hg

afr4/ye. Tes} xy lad jn, latore € (oadferd 5 wuediird :

b uSugtad Int 5% bectors vt eee ye EB onl.
Aecubate 3L. ef 37° He Ce 1M WH Od Uo egruak

Xo >IT @ec. aed lal 7 20 pf.

X 0,2 ore
L Lb ce
X+h

(gtd ta!) 22H ce. /. ag actly i niheca led

, hehe 0. 37” iybler fame. Rerreane hrbastice t

¥. G |0
J :
/ A ty {f gbae wa o Cq . IO re. Aypeceelhe glow erty
260,

[ok frat + eylat . -dh Yoo’ helece ~ Olle

Sthumak. Jardin ale. fete. My -> hbgin. Metre
foots ye
ofr-2.: Wak ua. et.

(D. Aum X + Barblecebrs - CLE ppt F tewepird ty
4). Do. = 93 Yo elestel.

rans fo fon 58. (3) LOL yd Supemalch L

(5). FuXY aoa.
Troon gp
(D. Sie a5. (Ad 4g AS. CLEA ppt crusperel. §4
(D. See $3. Do. hearer eller sector: L
B. See 69. MA 9g AS (6 sehusler + dae te) fe pple
(J. A253. De, Colle hesrerund eat .