O- prsshe galacloride Mer. 10, 1997. NO hte + gobkihes oF 05 % in 7 (u,) Dd MED \ ete Y- f pple 5-6 ryelalons iter l 538-161 + tt" - + &t +&- 2 YS? t+ 4ber - & tr = ee + te 5S W-d6, - - ~ = yy -_ _ nod Ho Re th HF wa, HtHe 6+ hut Ht ~ Le oe T Yye + HH 4. ++ 4b ~ 4 +o 4+ + +E g YS >. ++ af + wre ~~ — oe A+ +t ee 6 wed, host th tte - - ett 4 ga ~ ole, 24h, Bohr. gk en gecly cag od ny il eit sel nt LV lero 56é hhgrces 2 724 . qak lac. B-baak b-dgal + gel. f tr ++ ++ o +? 1 lb 2 + _ 7 + tr we, £- f +t +t - ~ tt £ +* ++ -— vw ++ 6 +4 ++ +i. 7 ++ 1 ++ ++ ++ ++ g tf +4 7 ++ Note Swe f ee Fe S44 rally laen 5 pallualr pen » Otgebe. UTTLIGATION OF B-Fhenyl Galactoside 26 Nov 15 1y47 Inocula from 23.5P15. 0.1 ml/tube T(BMTLB1) base. A(Galactose .05%) B (B-PGalactoside) C Galactose +FPheno!. 02h TIME:::: 94 SPI6 SIUC. Inoculum 2 > yb! lb +4 ++ +t 7 tk te He GHA ‘ 4a 2a +t a +t 541 20 2) fe cb ate + “ aoa oe attr + + j hac 44+ +t ++ & pe 7c +44 Cue ++ af od 8a +44 - ++ 10) fee. 8b 4 + 44 ? Lo alli bale trae by wbftigee undbalce ? , SPIT on ek robrose + + "a= O-golafordle”+ 44 ha om guilerbrers t+ Hie ‘4-P-gobtoade " +H PI?, Strale act ve (> Ogleccosde ENB: IA | IC. 10. bA-bC. 6: Aon pred. Toe volgen alae rtf ards chuden c prinol ga lacters B-@-galactoside classification of straéins Vy Nov. 2/, 194° Test on EMB agar using heavy water suspensioms of cells from YP agar slants, except W-28 amd W-29 from galactose MB agar. HB ly. rende W33 +++ W35 - W37 ++ W30 - W38 tt Y'/0 +t te R12. tt 4h +t 1140 ++ ¥53 ++ 3 58-lol 44+ Wey ++ WA3 - W30 + 4 W5S 44+ W44 ++ W45 - W53 + W46 ++ w48 - W51 +4 Wet tt 24 nrs. (a2y) W52 + All others - 36 hrs. W52 +++ W-1,W33 ++, YLO +, Y70O, Y53 + wo3: 48 hrs. Loker. fo absreT. fire Aten Ce he « gad aprTrinn as Ysa, VAI ws aud w33 ek He pert aclene , apeesitl, WSR. Tus tqadne ” tap pe hh nbd te wed 5 F-16l will Lae gato re thei laet rect 0 Bb a ahlinalase a Fue nly strnrtucl, of wren whbindy “Lae +b -" 42 WS 3. tile YS3 +4 bee - [ad . Vs : bec+ Lae -— bee /2f+ YIO YS3 w-t. fod- WSS WHS,- 47. SerngtiTid Coerre WS3 xW-1 Lace bd x lec /db4, daoNelt/- wi 4 x Y/o bee-(bd- x Leca/bdt. Retin feMh wv licsm Cecloie . So lube /Melbre Cones haplatinn polun Poe [0 1977. a. Y¥o lact 6. Wel Lag ,- bre. yp Br ity bel, ce. Wyy bec,- QM Nw hadivn & Lk Zs abi the Sn, Uae Decrboombabs Big let M10 effin W 10 okt low - pice “t te SA 7 2 bo — Coe TDs 4. ath 44 FE me lestore y. ate 7 HA ttt c bee - —— On PE re hocw ed fe, it from enna eat | C sa csp) ee vm festifor f tong fee 60 TREHALOSE*#+*MALTOSE CROSS-ADAPTATION EXPERIMENT. Dec.. 16, 1947. Grow 4-12 in T90) plus .05% dsugar 24 h. Harvest and concentrate to ca 101° /m1/ Add 1 ml. cells to 1 ml 5% sugar, and in replicates add NaN, to a final conc. of @ x 1073 M. Add 0.1 al M/10 phosphate buffer pH 7.0 and ,05 ml BromCresolPurple .15% ddake up to 5 ml with water, cells added 2 P 16, incubate in 37° water bath. Readings at 2h., 4 h., and 18 h., Neadings - unless indicated. Cells a Lh. {h. 1 8L. . (a Yet) 61TH B Sf +f LP 17 A. Gbacore Tlo)+ ,os% 1G horus. p (1 er e. Tictebay, A . ac . . +t + ter A cells did not adapt in 18 hrs. y uy Arde + ett in presence of azide, either to trehalose or to maltose. ~ tt M rt Av — 7 B cells utilized maltose in the presence of azide, but did not adapt to —_ trehalose, t+ - +++ " +Ar ~ ~ © cells utilized malbose as well as trehalose and glucose, even in presence of malbobtse. B. tHe ttt "© Ay - +++ Azide in conc. of 2 x 1073 M does inhibit fermentation to some extent but seems to ++ +++ block adaptation completely. yb RL - ER Tr - $++ Conc. +rehalose and maltose cross-adapt, “op Av _ ~_ but only unilaterally, trahalpse adaptation implying maltose adaptation, but not the x Jd He +44 tH, converse. +A - thr mM + tet Qeery: Will #alt-(Tre/) cells utilize oY tA _ ~ ade maltose if grown on trehalose? Tr + ++ ttt fA +Ar _ tht ~ a . - tha , _f > f . co Toe alate team he rnallearadag lA bujisech nie roon The inhibition of lactose-adaptation fy by Azide. Dec. 18, 1947. Harvest K-12 from YP-.l%glucose broth. 16 hr. cultures. Conc. 50/20. Tubes contain in 3 ml. , : 1% sugar, 1 ml cells, .1lml Phosphate Buffer /10 pH 7.0 and indicated conc. azide or DNP Set up 12:20 PM Glucose (3:20) LI. L. Lacbpse 2f. 4 _7 a MW. 1, Azide u/l00 x BHOPH. Gs 00PY apy. 3:10 00 PY, T:00PM (P lo = tte “ 4.50 - + ++ tee 4-62 2 41 ++ “ “5.719 - - - - 6,23 3.5 +t He “S57 - - - + 5.95 4. ol H+ v ~ 478 ~ + + HE SYS 7 + 005 H+ Y v 4.70 —- + tt $08 6 02 be a A a + +e +t Shee DNP 074M x 7 5 _ y / _ _ 8 . 1 . tr / v _ —_ durian setTn, 7.32 At 12:40, none changed. “ee Af, Oban vdacile . 10 Arde Horr: A ogpecadty rebel» f tf cevtte has ceil” an FU. pitts en + 8 [O44 poaglate Mt oA 4. 30, He . wats ae GO T ive thei the nee Wether Pep Hes te of iit add fers : acu, \ Ue, be baxt »% fe i add audalad” but Ht have bun presen ( Cfo rene = tM? GTM . ). nef shed wd oy thes ein wad tir nthiuter nek uluu pi wo mace fig pi A ws ep! tele Aut, ar {ran Haprrur€ Mu . ° ten the, vtec adkeirly x futalere . E46 Pee. Bf, (997. ) 4 bins Aeat (tree) + SM petebe ted glacere pete Le fothe chal mrebicoreondnallere wi potinihilee typ.6 §. lowe Te gta. Durl. 52/2. To SA Up. SPI. 2. ucose. Mcllore - 1P/4, FAX Place ete tte — - Titel, +4 Het — - OU 08 thane capable f poteny prulielere fut nel irullare . Safes 0k Mall wheal nis cpgmmnthy Tre + althowe WBN ci putaer « lebll Mellie tah nonpl wn veedinlefedley Phebalere. \an. IY 9/8, [ : pemeat LU tom | %o eublisia y Tm) 366. ule Hab ir) ot cp TEs with Ink cello, lnk 3% substak, M/ tohack wel MeL Myo plaruplalld BCP urskecaler , Substrates: @G, glucpse; Lys lactose; M, b-methylgalactopyranoside; and B, N-Butydl-b-galactopyramoside. , Ga, galactose. Grown in/tested on: Set up 11A, 37°. G/GA G/G G/L G/M G/B L/G L/L L/M L/B L/Ga 5 PN 7 t 1 — = 7 — ~ ~ ~ JOA 1S. — +++ — - — +e tH + — + C234.) M/S Mb W/W MW/ Pp B/< B/L B/N B/ 2B SPN £ a + ~ + t.. — - ne tee | ttt tte th tet +44 + a Tent + Yisemy Share bacon Pabflph. MH ok Malaitrrs Plurcerd +44 _ i bactont +h +h *— + + / ~s 444 +++ ct + M FEE EE * pit (lbs pabably be old for rage nclaytitn, beclein alls, s+22cuiilgaie Na frakinar , aide nan liset ocr avd oT rreeert alr enbgal | Uae L orig. | ety. (Mba way be tas od, Ged. Mall) a L astute @ L atepled are Maslaptid DB a> porly dir nde thar vonslilins | of Saleeyyere-th aslyp bot W1O8: Test for maltase and lactase activity. q3 Utilization of C-sources Jan. 23, 1948. Grow W-108, Y87, #56 and Y10 in YB broth overnight. Use } ml inocula into 10 mi. indicator broth with 1% sugar. Maltose lactose — 108 ~~ - — _ — + «ha plif 108 —_ _ _ a — = 87 +++ a — ~ _- - need, tarhor 87 fat — _ - — — 36 * — — +++ +++ — he flay mv 108; 56 . _ e voller . 108356 + i - 1083 87 1 _ — a 108387 _ _ _ Y1LO ++ . +44- _ — — By PRS aL ttt ayespt Ws8/N.. cells “herefore, W108 do not produce maltase detectable by the utilization of the hexose gomponents by symbiotic W56, and converse&x with lactese and Y87. Use small inccula from slant~suspensions. T(m) with .05% equiv. C-source. W108: fuse. P23. _ N24. P25 Pra Suralr vl on glucose - t +++ —- N-L- # fretralore. fructose(st sep) _ — ttt —> Mss } trehalose " — - tit -—7M-L-. sucrose - - - maltose - +4- ttf ~? M+L+4 Lactose - _ ~ Na lactate +e +t - K gluconate Hee ter v Y-10 glucose Hee +t. WwW /08 Y/0 Un 1% EMB plates: Nt q. p25 K glucon 44 He dE glumose — a yet l-arabinose 44+ Vd ete xylose fet “ he mannitol _ ote. AM: rare Aaclrrr — a +++ srnralteer - . He brett fore apseres phenstypes cevanimas -m glutear, rubles + Lealoae eels Wyo 8: Tact for (0 geht hee atntky qu %, 1998 WioP € Y¥/o pli la) +, So /OP, é ey ef ea Rie bal Btkorier + baste foon ch el MSF Teed /. [. Qiasek — — 2, Phew wader (Pop) = = 3. Dox 0r-% Heh Hee 4, (08 + + 5. OF » +r + 6, Via & + - 7, ¥70 4 fk + 6" Uplacmec onl, _ MA penal oll 7/0 Yio ot W188 urvhe. these cor 1 out /0€ ne OE eptes. rem ret, | net he le . “fot. eG Wl YS a L- c a e 6. Me eere Selah Demi dal £-autl- tr. WOK Clucorn co a + + - + + _ - wb! . Cc Phuconce se. +t 444-)7 = ~ - ~~ ~ wioe! = + - + => 10% . DD fnanalviusrt a oo > — + + Yo . _ Eh -arlnort (Oe a) om - ! , * - 7 ios F dol ae)- = ee +> Lt + > [howe “Bebe ee Yee i acme oud aglasTore ess, adore. Alen drglrs an Leeubmers @®. D -atalneree L9G. Mow ally Y/Om SD in - YP oq Tooled m giant. ee Reounml A af ceatl te dae BG | I byt c sary fo +++ dD. facta /%. +4 - Toe. te 5 nl anh er SO} te GUE DE ti i Shes ll boy ths Conernbalin, Metabolism of W-106. 17S, feb, 11, 1948. Harvest 2 batches (a.B) of #-108 grown in 50 ml. 1% YP-gluconate broth overnight. Test sample for genetic purity. A. (10 Ad) Cone. to 12 ml. Use 1 ml cells per tube, with $ ml. 10% sugar and phaesphate-indicator. (No azide!) gna gnafgl = gl gal galfgl Bu-gal. Bugal/glu aa li aM +++ +44 ++ _ - — - —~ ee ft - -~ nis ~ - Pst yey aa: 4 ml. cells 4 1 ml. gal. uN ml. phosphate-indicator for adaptation,to falactose. B. ll aM «as above. Cone to 10 ml. 1 ml. cells/tube gba glu gna gna-glu gal gal gnagal gigal Megal. Ways - - + + oo ~ x _ — JLN = - +++ +++ — - +++ - ~ igo — ~ - en “ - — C Cob), Aq. Wart, aud fertas: 30/4. Dna Bh, al X gh Kal A hiek. 4:90 _ — —— UW) -/08 C - Sorte Lhaghon hex 4 /3, (9VR / $0 0 both, rw. CIO ler f, 28 enh as /0 fo frat fe avbutr sty. 2 tol HO. ot

IT @ec. aed lal 7 20 pf. X 0,2 ore L Lb ce X+h (gtd ta!) 22H ce. /. ag actly i niheca led , hehe 0. 37” iybler fame. Rerreane hrbastice t ¥. G |0 J : / A ty {f gbae wa o Cq . IO re. Aypeceelhe glow erty 260, [ok frat + eylat . -dh Yoo’ helece ~ Olle Sthumak. Jardin ale. fete. My -> hbgin. Metre foots ye ofr-2.: Wak ua. et. (D. Aum X + Barblecebrs - CLE ppt F tewepird ty 4). Do. = 93 Yo elestel. rans fo fon 58. (3) LOL yd Supemalch L (5). FuXY aoa. Troon gp (D. Sie a5. (Ad 4g AS. CLEA ppt crusperel. §4 (D. See $3. Do. hearer eller sector: L B. See 69. MA 9g AS (6 sehusler + dae te) fe pple (J. A253. De, Colle hesrerund eat . Arse om paloty . hes eg ees ax rt 2.19 P LX ++ 1,392 Ss [Me ta. Be | Ia he o| x - fo 2 Youd! H 5 PY & ( Phe 8.4 2 & Aclonre Rt 7,20 7 (AS) a. hl ge 3.10 y (Alestis? ) sf) + 2.67 7 helo C. _ 7. _ g. 7. ~ 0. — Placer ++ 9.98 — Xt Bly core +4 6.24 — Wile ?? Larter. ~ 0.1% Prada Copy 0 thor + I abt 3. Cre dour, af Ua saturation, Boy Seah /6 | E amare ha Wer} bclre | jour uk ly. Ae Bch No yg St int. ch hu. py 0 heres. ‘ed a) Ko acolt FR / " MBI caret N 5. pow ech I Paik Coan. Aeon “fe 4 A pat 4 A Ae } im dO Sook. ra Frachin, | ve (15 AS) 4 Fa bw VU . v \ Vive fuck /, bee qt bes a utyct PEK (so%AS) / \ %y AS. Fratin, 2 [15 HA) Joe AS. U LTUe pph . ~ hey la A ae Pye. Eaton S . / | Hasan : luk OS nl 2. Fratton | (hy sake) Ab operant 3 F ‘A Ch sat.) Qo f. FS Off se.) hea cg. FY (gah) Lear pS Reside fle, ASeal. V, optelerend Nesng wth 5h lulose Wyle 27" )6(b >/20 L30- 2.4] AL afl. wR BT 3; Ht 8TH NS th de po. IDSA 9.4/0 8/ R 13.Yo- 45,70 2,30 frie uit ancforoy R[20,. 48,70 - 16.70. hivlaltef. bYecer, o. ptnhy peur (bet Mtibld anes the “anrrtable ceo dbere Hu VfAS areol fhe A AS pastes. brtinin te ayting thee prt ‘ap ot WAS. bet ys f ad portion’ . ~ aie on Pts >?) o? st 4 si + 7! wk I, hhelonr Se ( Aare Duan. Cc. b) .4 La fe ie 7 _ S. > ‘ _. E ode S11 Santis co tall hasty. — 2,93 PAS ¢ ‘. = + 9,90 Nh. cha 0 Muda bt “op: a Ake —~ cmditone te » haat ‘o T Ly tare ling + B aindt mee 16 fo Bn soph. ¢ AS DY ter racine sretamed)- CN) Seaperecatiod — G6 Cc 2) dRemTebh rides offer esterprurenens n't O vil red Cy 0 Cc >) Non- xdomundebl perschice . ~ Cy 0- Id wh reuples (in Binal Sep covngonse unt wrtrkewoeslone. >, (243.2) Yo° be ts ZL a Papasln of lactase (Babel, 2. Y62 - Duma K- JL mM j2Q. V deg ae { 0 Facrerg fo ae stg necator, . Afar LIL. awead is, lap es (Ur fan,). Fashions '. 315. packs ~ hth oon HD, Stack ftetrcr , wry vw, phen Cmernedelg ye of 97° HA 24 Peaks 2. Yq paste . fl /000-N fectie sie ek, hime + 2d fuel acetnn 2 Kea gL , ay sceiceitr, cpt PIS. 4g. (ancl deg ') pork. Seungernl Woy. ees Pee ml Hob baer (eat ah w 16! b) : wk serpin ein, ( seiny ; YWo°). ee ard 107M K Mindy, Akh 11.56 AS (hrs) goa fudiradet on iyo. A ’ seperated B. Tol . Tod seme d tac I62-YA Cue Ue Vovbbe fo vey be “— ; fag nearny | a a V6 2a P28. haf bb. hrbetanak Cadkdls for in C4 Os tabee wap astra th fel decently fico d) 120 ul Alt chen . - tolulic, eathaly tar | YT elle rae nk sennple. — Worle with the othr 140 wl. a a AS. CLT ” 80 cae 4 oa Lf oo wndin. rc ye rot Cost wechgaele —2 IGA. 2. p fr ond) — (Redracebet mv 50 ml.) B. Aeten bin , Ol mk sonpleo oy loo mf tance ) Qom. 37° A . (Mable ay pats! Laden elle Liladaitd? | [A bens, nae tbeben’ J 7. Art. Repeat acme re Olwk. we M|i0 Na peasy weal) No frcbody bavtere prepuce Mah 24 tf | Joke, ha 11 ns W2e20. + Macro, Ub laa s lackore | (A) (6). Mele, 37° 214. (Mlk ankfem ). Gthed a3 Mong len bolle A bf Coll S34. hpet rom ©. [Beye AP nemart) AL. 8 aud an 100 wh. tall ut Vink. lure BL. Pq. gut am ok Sip lteter stale broffr 1h bh. Theor nrerhs eatin vod 1% potas . Clluat fl 16. Stree \p31 mi afc rm sheondennny fal, wet rcay it 2. aol | Lkceay bed . Hei ) yak —- whey UI Mi ml te sortdig sth / 2/0 Agetore. ) 30 Ke Lin . 3 7 ° wth, GN a tegen aed A oO + de ~ deg the certhin . , J e fee y 0. - ald bly ful tee shan, bl 7 tr. he fo nar le f . ~y BR? 1-0 + 0.1 _ 46> jo - 4636. t 62 th ty bon VG “iS Mbrasclot ppt ho. [aeeperalcce mecherdea Lox hrtatele af YI? J/iAr - Xa: 250 pbeliace tolore's Record Ww — 340 Tad a YE her v8 + 2S plB , xr00 3490 Seon fetal Sbiepae to vas # pct, al ¥ 36. Aly trae ++ t+ Dbseornie + + Holbrae t he Leche ++ - At 17 ths utah wv prreler b W-lo§ , brcb es Aachen etek, ming he aunt con ST fhare Fhe ghccryy mare fp 6, 1470. Mrabere . Joo paler x 270 = 30, [eo Vo toGhA wuterile Yd” /. Jd. IS. lt 17. T parent Mane. errands W- 340 Mo. WwW -d70 a1 OWA hth am 237° LT? o yor as 37° LIT" | al Lae VP of Cobbs Ahaverted fom l00nk Ana 37 [bt tho. = g 37=A ¥Y¥S°B (lls fron IP bee = I. (SOunk ue Lil Ho). Tet af 37 +t af >> of “f We 3S AM. pa X. ST ea YS i frac + +t one 2A | Sha. +e++ ne ol tes “ H+ tA ine * tH A jlo _ 2D flaw —_ YP | bee — _ Af war At ns Sinales . Ix vy, 8-10. 172 Lactase preduction; K-12, lactose synthetic mediun apr. 9, 1948. Inoc. $x 50 ml each. K-12 cultures into 10 1. bottles (2) of synthetic medium (v. supra) with 1.5% lactose USP. serate at 37° A9-Al0. Gollect in sharples. GT prawn dannoe A paste ln Jom? po ay ~ valine + nl pleene = POAID. Cahn ey pe ISO CC foray’ Sere Wow, oie oe ve oridy | eth) LX 45 AS. ph. | wm a ft tal ut taf ot clo iPro ened + + fhe tpt Artavrertel. The cup t Urelein oan na wert unl $00 n8 tah Be ey: AS atled. Te pli ell fe Se oA) Presid wath [emus (2, deasalet pf uc Sdaltto (GB). Whales GALL 4 ylbur aigaunh ) AO. 0,00 0.0] -©.0f oB. 124 7.34 10S oC. 1.42 44 YL Cro 1.38 l, 34 c/80 41 Pate #180. fy Ho actenk | /724& ) chbon f adap hibon ba ems aed Aagprecsts yy Pred v2, 1948 Gok Jule co rake b 1) ce. Cbs Ine Fro VP - ov VP -lchra | el | nih, 56 oathnn 5S mk Cony. Ch_ardats Jimnk Phrplat. (Aedfe (Wi0 ¥ lea, * les asatictime t (reg hadhny capac” +t (ng apa fern L. frre: = +++ ttt — tee 2. OL. tH + + $F i t Hee 3. Y. He +H - + aa 4 V+lL the | tte _ é pet. 5” Asp. 7~ g Mommnuchstegd? — YY + ™ oe Hofo . ~ : +++ ~ “ - 7. Arps Holle, He 5 tt — e are + arowuttoland th Oe / La bf i 7 . r i 2 hp 4, 1948 27:9 Z:Bo }/30 [ —_— ee ttt 3 ot: f —, yx, — G —— 7 — 8 _ gy 4. — 3:¢7O 3:30 + Ff + ttt tk 4+ tt SF 2_ Calls oon Yor (05 Uf) mL Wriear- Fe Junk cebl, wr foul. Sark tebe oternd ! link Sy /urk Y qo Lott —tldS ot I? DOK MM, dren dn 770 WH? : The (ecgprsitinr murtan 186 uj) - 340 and W- oF2. May 3, 1948. Add i drop iwcula to BCP-fermentation bnoth, at indicated temperature: W-340 glucose lactose maltose vane. gluconic 30° th tt — +b + it — $y 45° a ~ _. ~ - wen W-382 be , -_ 305 tt te tae — +h ° ay qt — - - ~ Hee oO v L a“ 45 — ~~ — - ++ free SPD. Feet Renda 8 BAN: 15h. Tire ore batt, temipere tect put hurls | W- 7 it nf Ah dank Fier itn adie fapte pelle and buctad ad bacfewe, /94 Pruett I3+ \- / uneeefars, or recy) Foufe It anf l— on aval feet ae syle ote [evanesetiou nufercls . 134 Vay 4 WE. Us. | dang vcorcule fo fost ye beth eliza 7 vical prrvtilion bith ACP ao avlkald 6 SP 1G 1 4+ +4 +4 oe Het fit te tE W-108 _ — — ++ - — — +f W->/0 +44 He Ht H+ _ — — tee W- D8. dee +++ ee 44 — — ~ + bin b PY. [Weiler 4 56-1 rn ren those ( (F-aabs 99S = 154. GAL ~39b,. Hl nsadhings ahueerl . Le. GAY= 6db. [oO Critical temperature: Temperature mutants. [70 May 5, 1948. W-340 and W-~382 inoculated inte BCP broth tubes at indicated temperatures: 30° Plus on glucose, lactose and maltose in 12 hours. an@ galactose 32° Ditte. Inecula from gma bréth .2 ml 33-34° Bitte. 5P 5. Inoculate W340, W-382, 58-161, W-108 as above. Tees. pevaltee Jhcele, a 9A6 16h. glu lac mal gal , ! f a — eee THE hoot Stay, BS a geh a, Thee 282 — ~ +++ — a $44 llc LOPE LE: { for lear 108 —_ a weer ~— . t+ t+- , ( i ake pees }- 4 54). Ny: Wa 58-161 met tt op )) ‘ a te ‘ — if "At 36% W382 2a bac r Bly - W327 WZST: adaptation on maltose LP May 6, 1948. Harvest cells of w-2b7 from overnight cultures of YP-broth. 50 ml. / 3 ml suspensions. A)- maltose 1% B)- gluconate 1% To lml 5% substrate, add 1 ml cells and 1 ml. .01 M Phosphate buffer plus BCP indicator. Incubate at 36°. Set up 11:15 A6. A glucose maltose gluconic A. — +. | — “> hee — = uv + B — — it v . ttt + - = To 1 ml. B cells add lee glticonate and .5 ml 1% triphenyl-tetrazolium hydrochloride. rttey dey td dry) Smee > Cphteyenl Shady od 4 A (Sate: ot 30) 401. AO febersucre ERE L a, ‘, tk. se) 3 RO. a yf 3:30 6PM, — - )724. ef a6 _ | 6 / “ss J dew W2FL 4 yr ¥2 bath. te « " f9~ coed gtictore. Seb “7 ud ft 1 P94 nm, Jy Jove. behecrgue ae Cea W BP | _ - "0 FW H+ te seal tt Wty ~~ * 1130 _ eee, TT Ranpoertin tant? — fhe, rxraes 19> bure. WedPL, We3¥0 und Be SE-16l inte PCP febes af 33° ¢¥o° ar ncuraked . 6Pé. lot Rin G47: 1Sb. 33° 0 acnwre Nawelel Eructe Sebvlof Nasrnare Nand Fuutere Sabihl tft ~+ vam t++e — “ — —~/% -4 — ew 4+ “ +h 7 tet ~ ~ fe < —_— Y _ / —_ ” ae tee ee te a yee We pee’ pep / He + + “4, af ~ eye uf. te Taf . hula Ager ~-Acaberiori X- abaphtrn slipfttcn. NY ‘dah 1918 Va cueet K- Nn Po LO a) ase (ret | : b) gala hres jogs AMT) | Dat "4 Hee anes oe ° . oes foil —— tubers ote Aeceped a Heo : — OF + . | - +H “ 8 . | | } +t Fees He | - ye tad- 1:30 lot | (2N 2A catece\ bee 100 [Adaphotin; ni prrtnce fret Mealy a ret x golectose | Cotun,'s EE £ - eathnnerst aud d- grlactess adepled cols | | she Lied) y W~382 195 May 7, 1948. Prepare 8 ml cell suspensions from 50 ml. YP broth cuktures (YZ-sugar) Cells: A: no sugar, B-glucose C- galactose D- lactose. Substrates: 1 glucose, 2-galactose 3- lactose. or at 40° xiutx After harvesting, icubate celhy without substrate or bufferd at 33-34° for two hours. Then (1:30 P 7) add 1 ml 5% sugar and buffer-BCP L frog 40 A B =. gts ren: $1 ge BR KH Ht ED ee - ¢ 3 ce t. ow! + =_— wi. # mop Mae. ~ en W-340 Exactly as above. Cells: A-glucose, B-galactose, C~lactose Substrates a& above. Le ait re «é Os . ee ef fos roles Av Rey By GO coed wb Ven F4e) oT bee eR ay) oS een Meal we me o : oo | ee a a i ya get Qhan - HA dg _ ov ae a 8g bY we hay _ _ 2 - = ‘ Af... oa we Cel Blaccrgpyrare aolaritive oF 89) bb os predicced daving ralectort adapter 0) 2PM. (r0 “3Onmunes ), eae 4 fen, a Bye Peunernaiy 1 9/95 19 Tectl for shebrbh af Yo" W382. pu) d¥0 ool Muccore Yolesto backrre fort Aehat antl Purest — Yadecbers re eR Lockear. bee eR ee at Bt? 0 ecg peace crs Aha i eal oP a abvstirce 5 bd ain gelaslore anal lator eclagild oll es Altes | @) Macey names <2 ahaproot af 37° (3 bation 2s pine teble at Yo". i Lenape tage fon WO are) W962 sides Hhaaaist crpaatle enndebis I [dar eubates pork ohbebh ?f Pelt dado liek tacts 1 196. ahrwneee f dubdale of “0° Way 8, 1948. Grow Y-10 and W-382 in 50 ml. batches YZ-sugar broth at 34°. A. Glucose (2 flasks each) B. Lactose (2 each) €. Gluconic ( 1 each). Dispense 1 ml. volumes to tubes with 1 ml indicator buffer (with and without azide)a/ Yo At stated times add 1 ml. substrate and record time required to ferment. Cells: A,B,C. Substrate: a,b Azide t+. — ; . Aat Aa- Abr Ab- Bat Ba- Bhr -Bb- * _ j o -. “of \ > Tame suboh fled): oO Ts HE) Hy Wher Pty 1,20 TIS 2 ar nf Ht yy 2 do ty Heic 15 thy a tru be tys Hy, tas oo ‘ wag ae é ey , + y 10 60 tr FO 7d tay FD tetas Tras FO tt {20 {le ve he: ee theo fey tiie FEN IMG / tT Go. +44 ~ Toy. try “of » ~ +E CO Fish ss” - - tty FI4, 30 tye Hye. Hs +42 23D a rth og WOrhy - L 30 py vs tyr the Fotyr OF tur tye tyr © > jbo se) . +t +1270 . hl 69 “ys WT tr %o ta 12 F277 ros oo Re 160 ribo [2s [yo +rspo- /a- / f 1 . Z fie —- 4 we \/ bee Pe | to > lo: dS an is Ss 11; 6 ‘ ye > way Go: tS 120 : mes ha hat for NS o fo +4 oe 7° 4 / : i fat Aa - Abt Ab - ‘Bat Pa - 3S o WS Is 30 30 176 | Bhr Bb ~ YS 30 . \ 30 30 IS ds 30 45 3o 110 60 vo 8 go do 30 Bo sh Ne YO 1l0 >Ie bo 30 FYSLJ26)30 > a *° ¢éo 30 YI -4 3O 60 30 YY NE 2e 10 <0 Yf-122) SS —_> Nall conti? on fol 7 W~-d8Q qlee liucrr yaar at 34 °/ Une an calle ff 292. Adole desoudt puvtut ther aerlbd, ean tice te fetnan cneedeldiy, 982 ducceroruana, Peppes gic pd “2 “a unrrhtlte co 6 ghaceysiane ty hee LE ie, forse Nenpeenlice drreerloce bee ~ /47 Vey! 5, /9 SG. hoc Lac -M2xe- OP fevmioblen tales engply fone sf. Honk. #5 SP) é 30° 375° 4a°- WY, —~ ~ — - - -— — _ — W-NO -— = - te ttt 4 te 4 ttt W905 = + ++ coo HR Os +h Y-/0. +h ye og Ht th ste HOH Ott Ot 0. V4. he = IVherr @) 7 PI6 =2Fh. & GA17 = 34h. Coty bechrar wo 60rnL V2 Lae froth ells beecled) i, fork 4,0. hceeasuy 7 ‘ofecl ‘ala An “(| Leal dnffr HTS at 37° upc t4/yem. /OMyus se tier wi . Cosel Le : cere. “lay A> Ylo Az A 20 »> 650 St an bd Fh Wt 13 ca SO Jf 065" 09 .o8 oT] a hc ied 074 ea 10 ,o] 009 008 orf op ,036 ,0/0 ,025 < i001 007% 00" ,022 07 — 1023 <¢ & 650 LO Correction, = rv; n”. 650° . At ip Hae maanl,, Yor FAA 10 ms feats 5 bed Penge = 10 ey Bolinns shoved ee ey: Ab Caucryp aarnd 2- A eeeell. Ayo eso Nye DV ese x ce A —o1" Vos 334 HSE LS’ “| | alle Hf 64 h- :7F0 523 ONP, CT. Mx coat bff alse. k= Y20. SgG000 1O70~ | C. D. t / 1065 1Y40- ADL L 7s 4 27 p- 4 ,272. Sad .YO>o~ BIS G 6 8 18- 8 isi 10 614 10 6149 4: 420 A> SOM eo .20 ,O7 (7 1 oy [Omne rm WPS my shan SHdS vare CPE SS] 6 Or + cre Ayah evees nie thls SA 4k Auger tuber’ tlk ofS ffs Yrs traf. lL. Loe ke OPS 2. Lew OPE 3, Ma 4. Mak " gs. 00F " *Oiaiaes Hea tacgs at 37°. leo Mate. Dino. %M%so Note. aulAhta neblgen gtierea. 4. 1032 oO te tad 3 080 o/79 | % O62 ° > 1290 ofS U|50 celal buff. [$20 ¢ WPS a 28 m- 37° Molen) Pgace % Mead ok. — Fo Mu bk 4d ~ 83 Phbletu SS Peat - o£ a 76 - : . healbrwuere WRI ow - S oo Bt Mahfterr w3B ~ Ylo | a - PH, Oo éeL — fuck, 13% 53 .2aof Ld “Witte Sa IS - Whe 164 S deheteh 060 74 dobotes 194 24 nueblrert -C00 — {ed Seph V5, 470. ie [Dk ey MWouH warteracted ry Ful balabanypierer Ne ond pas above thue el mk ‘iio OPS waradhidl fle, Hang. Cb uel ar t or - Color Aor . £ hep lac TP & “4 . lmastiale honey Hb f - esq ef 2I- 19D EIS + on + » obey +t wood 4 tert, + ott +t i: + a Tr +t pit diutted 4 Yrlec(L) andy? ty (#) a: / Addnsolidin systein : Ak cell, : Ank 7% sagen mts Off t [ink (seapgalirunt tg, Oe tree + Atarpinal teas arncd ws Yuk Yo Toate OPE om ype above, Nuk : 9 owe e+ dnt Acer iat ‘ad Us Cn cliban fore aclaso tet, 303 ey VF. yr 1 wll Man Y2 fla C donk (so) MA nd celle I nd pegace 1% mn Ms ofc pl TO. Behl Ho om juggle Sil whume BO AIP dy 5 shabiing af 3° / he yp WP 9 etn , buh wee bitaf olin Lv ” — Tink. Hears /© aanel reat % OLS 3 “ Pepe hare. 1*% ( yf 7 Y. yt, ./% Ack bebe o) ayprnct mal super rerio x nO Atyegre | game bls , arch b) the agent 6 Pacerrthalactru a linker etlat Yo. 7 —— 9 laefree Hb Cre, (1% A (atvila) (hls) A.A. "/, L. ( ,/¢0 ,207 (77 /wo V. €xlr,, Hegre + HC 499 at274 1.79 233 ait Lifndah vheikelry Ce aolayo- 4 SSI 2E2 SH 710 1.98 = 23) fale w 5 ,0O2%2 ,reo Jif 14 6 rzso! 90 oO © a vet Pedal supplac en (1, i! on, | 3// A ic S D. OUR, yt oO rH 2 22 Y _ bec 230 18 - Mega L4g 2374 add Dee 319 3) 6 oH (NPS. VVC ZIG a Ae Du se Sra , Bena? Vir Got f . Dy WO ? xebra had LR ae . [fins ~ g. 7 4 ney ix +4 co) D~ B-C A . A, E Dit = iat ne bre ey Cf bane f 9 Z D E © 49 OV Fi heee. AD | 34 24 y ROX qs 47 — 69 A> 9 PO 246 low 4Y§ -— 7 AH 535 Dad 04 Wa — 72 AS “ 27S Yq Qyb 1730 70 GE + 103 Ae > py =$o 2r2y. 186 10 _ + )63 Rdeeur | 2 > “tl Bd 76 33 7 §2 Bhohon Mo CO 2B7 16S 6F + 10d Mafluc + Tos SSG 232 12H $3 ~ 79 Golem poe RY 299 rl 68 + Jor pol 5s ST) 232% 139 Go - 9 Bye Yh 3A 2D TH TD “4 ., 2. 35 HO 4 uid as 99 A 133 bon 2 ah BSS 298 NE Bd + 42 the C7 a9! 37} 226 161 (67 —- foe shee TS IT 207 }0 9 7 2S Ho. ash gy? Bee SHY 176 ++ 263 HCA Tey, ' Dili FEO Blu sue (78 + 266 Whee 9 Féo ey 237 1FR2 73 & oF S/f Prune fled * (apd, 3/2. 22, 1948 Se al wile Alec — fl~12. A Do Chey Oc) EEE %ogfbacls) ho = 2. BS OF =p ODS / 00 2 Me 3490 Fires 2779 Y2t isl 3. AA THE 29 GSC YOO 38R4 ly ALAA 2774 320 2 276 HB. 5 AA -AMN- 129° 347 6 103 (80 3 - 6 " Ay 244 8 ep tte 257 116 bet 7 " AY ? Y~ S2o 33 427 80° G " AS 2@Yl For az aia 98 9“ AC 250 Yo 22F 235 Joy 10: fag eHeth, 237 37f ay 1sé 7d | 3o hk sack AA 4-4 Sh ww: 10 Chip lechere. SB). K-12 gon hss v1 Sapatbelic 4 Lackete /4y [ilele. « 259, oly Ai ceed oe Orc. 7% fo, y Coffe | a Lipqleepen flewsanee (ack [ruse deco MV Ago brahualle bali pl toc alka ftp. 3/5, opt S, (904. AE prc in Work Vr Lo rw. Pervert lo” See. Wns acchloys richer Perle Ps lalny Zhe YP. (O wb. [le within As etioreA puguevalic dilabd t uc @). 186. Kenanbe (Ip )unwdt Rk bbe Conk tv aks ta. /0X hadtal 008 Cee. 05 bball ys Ca Wehr Ho X joel Hore WPF 46 WOuereca).. LE, . See 3/6 FE. Arcane: A B c. D Aef- ful. | coF *40 293 143154 Yn 2 ooh OS 20S eee. /0 RB .«.r~w 5 87 266 254 25 BIYA Fink y ovt o43 O47 40 Of nk o o/o O27D of] 90 O08 wel. 6 022% |Soo [50D 150% BA fink J coe GSO G80 980 an § coO 290 290 2,900 1 360 190° /bo0 (Hs? H16Blcll) . eb 10 OnPG. O79 §80 809 NOD fk vy. tea. nk Of2. /2, O Oo. gly ase f ; 7 m aon fo. _ — / mo ny G, p“f- HW in ) [mk Aaa ‘ = Cec Lite ARE pe Lente {le . J y re he Ce iby . ' i j a vA poo, . rt aur .COl me Eos ARLE KAY count ‘ey face mis). tacts ad 4 a, alba, " thy fh, f tif J Paddy ‘ ft hes A “a I “ope feos 1 [Ad a ee “ tn prot dod us a dL : r , } . - Ff, - ft vet / prt ik we waco, hh de wat He eet! balk 7 et é ren cf on tf eb ADL fen. ete ith Dw. ie dept 6, 978 A K-12 ret 16D ml Vie) glircose | \ & HW. (Ambre) (a [: 10 Beko. © bese a ( He. 3149 i |»2$ od. rs fiat ow (0) cublacr Ao Reno, HC ellie 24.6 GH. oe re. O te the oy wher ea fat atl, + Buck To): talectore i hoe “ls ath 8 pep Rerurgeimabirs' Yall. pe , | | | ot oe ee M704. 201 fy 2.710) ce AAS 664 as 72-C 3. Tle)bace - ful H.C. 256 470 co 1260 73 (BY. bac buff. 177 1649, ~ oy 184 475 5. Tlo)lac’ NPT 7) 1S Wee 170 6. Tlotac *t lwlte WF 483 4h. ITZ) 168 A. 4. A. ff 232.279 Bh. 5/4 1239 1,20 balaglh clink (uocabapied cel bins eval © series. SIP Aiph 694d (va) wWit7k x WSF on bac 1, 20 Ceborrein ttiials ficrr- Esathonnton 7 gehen 316. 3/9 ay leat (3/6) empasth 2900 ufk mn l00ee or 2.9 x0% ule. Lb Yrs obs lo badimoke puree SOL (se ESOvnk 0. CLS x10 ule » Bese? 1500/0) "3h" pradion’ 0. oe Yalu of 17-84 tach u, az bet, Tt pg hati pact, hope ea Dak Yr fo,* @ 5 hop fad Acl. bap het. hege om | (st). $.00 , ng er? 2 (het) 5.00 390 oS 3 (Yyset) 5.00 JaY 622 4 (st) 10.0 (Oh els” S Supernd. oo, O65 1S. ya Masayel Ces ep awelivt (Of and. oof wl {tempt factor ¢ ll prow” myo Poy bff. ‘ boy yah. wis mo pee ade 1: aol efi tan Fe bare hase hes fev , 44 aa auto tthe ‘ther - ; ye of oy fl pe ha, Qa 3 ( Vou ) — My AS! Myet) Tele py etn, Hise vebiale Offs Bons 2. To nag FonL (1. ¥ x/0 Toe) elvearniuactes atid, amd fell sf ty asad iene 3/913. Ther tee edlinky fated F Talatop 0 mg /duk Vevep bale effet o phocpleds w- tebes 320 lot t prpeclin IAA ti aecspalid an H/s> lake bec pntS (Hikes derinine ~ cites accd) = £00 bnffer), wuad thorldd anne a ca. [ho (« (584.23) 110° yal. = Yo00 caf wl. 0 yl, ula vod phage Offs Wise prs. Pe 37 7wll EDC + [unk Me + lnk | et t OT) °c 7 . 3 " 39¢ n 80% EPC O12. nw CQ / 2 1p . & 12. onP IM /¢a00 m 1 £0C Gest a, ne Ore a FO" 73. Se 74S Uf. (Tr lek.). Epc? Poy. LC 30,19 44. K 12 an A) Th o) l:low 4, OE. fr ety AW p 4 c CS 2- a | 176 LO > 259 pd 3 sbL 21h { 760 291 f 169) hI 2 (67 we 2 1f6 126 f rY 7B t 4yso 81 2 1790 s/o 3 226 S54 Y. 29 772 af [etn ne, . $ - 2 pi we Water te. ~ ape é sd peg ler Sl Mane= Resaprerselrn, Sint o. Tubb. af ge Sp wey ae wo. Relak& A itn 6% LET pet Oh Ce ye e f | c. Swl.. | ar° J07°A - rer lin cell, a Bink tbe . O bang ‘4, 5D 1S 42 %Lec. CP TI% )Lec. & Ox porte O, fy *° 2nd GG) +" BE AA. BB 18 710) coll, Lh wat atapt!! TA) celle une abnnbald by 7/0), + furl be aunio aes | Use A | 2S, i » 2 2 2 4 / 4 AD © 241 is Us] 7 RY? $ 2 /f q 1M 1G Boe Hf % é a : Vy 3 os oe ? é q of 3 j} MD Dey HH, 43 I a ? 4 SS AS NES ight . 17 oe Waa de MOA go TH OT ‘ nr a a i 4 +7 137 jot (FS 14] 83 169 [SA Ms, 1 48 OL 176 208 A447 4 08 199 hoa 508 54 b= BC E- Phe o bar — oat |20 > AA 96 AA-AN+ “4. es hin 77 " st (waiBorery! 149 AK- “f y 13 - A, (2X - nth 139 — -ca xt 110 Ah-Ads FL 24 + 17 + ¢ . . le Ad - dab udide ? diuo ghee . 1729 “4 hap dtd 167 wth Bod HE. “ . ’ Kr fact Buy, Sept do, GIVE. Ec A. Phrphele ys tak | Aylin ca , aricrual 10 ah and) 4/4000 in OUPS. gop rk 4 lachree 3705 ase fo fead 1 mee nfs Pheeplale 222 2. Im2Hls ectieke e2 | 3. lmk tac. C2r2, HE costa | Pherphale blfe. { — 1S 9 2 Jk Epe Oo; 3. [gS Men 190. ms fess ey te te Gens tty Ock.|l. fer® 002 wid KIA m 6 pllens tnffe, cach of Ve <2 /. Phoephale 310 fetes mi nr plrerpActe ina 2 Plycrinpherprule “UGS appre A [Ac of fed ° mt >. * + Phea pb 477 , vo i. 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Rome ehcrveat. ba. “ye jheatd b3 wa 240 swt /0 “¥ ~ jo? regs Biff: a9, “a: Atty Prderree {jet LIGA. pher-atiay | 2£ of BP 1-900 declare. Le aaetle of 219A in fs ta tac ttt hi fa- Yl De lopedne * a 660 Hjo4P =x Jo 670 x 3 230 r tt OF 2 Oyo parte LITA, Suk 1170 . | at 274 Lofiak. 5 3 yu. < JO Peal. yrualle , Jak 219A cnuspold lc cq. BxI0 tp Phocelule , (-€. 219A ea. 3x07 fieipla At 6: S20 vosef 20 dbend>, parefo? , 7 unrfee nul, drs than fo "A7 Papal ee palf 2 10°" ye ha porsills govterart: oo Oye utes. C apa ba parndthy rank wh Honbne taumteentan . & ~~ ‘ zRIGE. 2 ~ . : AlGe | a Atagma to Ma. Copper a |: Jove Dero, Enya vat. “et ' 098” 2 140 ¢€ N/50 3 laf . Y 279 M | smes-) ob0 $ 277 Vv {100 6 290 Y ft $0 7 #172 V/10,coD. At. Hed Na CL at 4/50 CL abe ; get's (at Aline OL Celecer! / | be ) heene tice . y eo 4)’ Od ¥, 1198. Aegina conten Ool 60S yk DAIWA rude | ) S in H fag Od PG > bnk KP cy suaffr + luk iso Na, Shy “ , (0¢ iat. E $ e A. ool } 37. 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YO wen - . 4 (pes ye v 4 tS PA 1 ohh pf! on pc 1t2/M yy py ys © i L MPU/s 62.9 1B 7D 2 2 8Y.7 o 122. WP 3 10 Ve Fee 83 &> 4 10 1S 3 S| oo ny MP Me 6x9 13 an 83.3 9 124 [Lo 13 > IDS. 7 gf FO "Go 208 3 5] 49 2p KP NfSo /10 [0 93 33 22 5 ws” JO 64 SY 230 ©«j0 303 6 34 33 z¥Y 10 417 0 2Yy 24 & Cf le . J 3 Oe ORB At n7 od 160 os - a2? / 7 ‘S OL s7 3f 0 “76 2 0 2) 2! f Mek te Lit , — bree ll : * : (o Letyarre pop 4 5/9A ) yf |b & fr a 7 " “ee tor te, wees ale, . Abr et 4 t, ped / wate te BE iret wate io Finged FMAM K anne Ma deafrisl alla thee Ky prrmnch, rms NQ. 340. -M DIETZGEN GRAPH PAPER EUGENE DIETZGEN CO. PRINTED 3.8 TROOIN U.S MILLIMETER doe. 14 1478. ret ol PC MaP yfee. Tr oF A A / —_ 1600 / 14. | HO mms! ~ Wo a a) I Ib? Ie: 2 5 - OT 2 128 3. 10 \ fof ! (00 4 , | yf. Is” Ce 14/7 O 134 IBY é 7 | rr 5. 205 x IG — 3 lcgl roe gs 2. . - KP tfzo. fh 2d 93.5 0 Hy }o7 Mer QF I. © 7 i 13, 70 Zoe. I 50 44 7 We IT 332 Pe go 36 tp frais 1S: 20 28 . 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[aid h aly. Bur | cache Ah -12 vi Sllae) wots 2YL, “aluveel A24 f Yeelf Sbaneeo hows Sep fatto f0¢ aria derirester. unt ch, od Le tothe, yo nn tp andsnnd CO nn, . pa . Seytemnaterd, yet al 47 (ea. aah). A). eytad ( 2) >f7)- 3y/ad pam ry . deape XL [iP vr op “ wks “ [di 7) hin F Spat HL. a a ~ G} ; . ’ # ‘ peg tr ke, le V t Hee Me 7 4 ey i249 Jagr. ( o). = 50 ney jn ce wth c ethan b), fee, eof ek t vee. Pas Mees . nw |x W, p (. 5 7 j2.d an /o¢ D: fon 4 \ 50l v - 00 dy/ 245 Pe wdk ee Th 77 77 OTH Soe Coe Uh SP? : i] 0 11 3/ oi iM F. Soa fon de i Hy 0 th by LAL ri ca 4 4s £9 fh - A z at rtin, | UD. we 1/3 Wile bury crit 2 pt ht mepacake apt ard rcedrrar’ 3 ? 7a A 7 mM You 164 y20 68 4x0 o7+ 55D | b> $50 Os ESO 64 so br (700 60 14 ipo ,J9O 166 ,22 elf “+ Yo) Bay ethitey bb /¢+- 66 2 ° § Lee), peel hag ours (2-8, #1 |» Wb) Wg, wetperle 14g. arp wheat bby ed eel lb Fh. hy okt | [Om0 -. Yall YW Q ww Aero spit: weet, Yo7 F049 | R YD. ust #2 bec. aveeelf henry ger. 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Bip Ey 2 K, ! | + we j ‘ G73, 3% et } He Oe repel be tyalated Cad a" - 194 it jos” 87 139 127 103 i i Ts Kinetics of intrecelalar ~ Ky AS x/0 K qalaciosidase. - 617 MH PHTS Hho a 5>- foe ans: cogee - me . co an . ‘ , J , :, ee . eee 8 em em ne ane rate oo . - : . . ~ - e * \ = Ye, > 70 a AI Ahiacellber gelector herve 415 YL hath, Relecceragrd eon'bm 14n Wa PA ftco 1.5 : be Yok \arl. ‘elt, ¥ des pon Adhd or oe t 6 cartel Hi /se9 We Le Port “7, l, ; ; 8 “howe walens 322 | 3294 Oo 374 3F/ 607 380 | 390 00 37] 380 oF 32é 439 0/3 res) 74y | reg 163) 769 coe oy |f3 Co 2 Via ao 2D Kn = “nS yx s0h4 393) of 4] OLR 3) 0 3)? 1 Go8® | 18s — «88 cos We 7 oo | 087 | 0970 sol . , —atle oe | aD . aan osy iso | ¥su pe opm tin —_ AIS. 4/7 “yp 318A tar tA. Ty gal | Hi 2! 00 +, wie (21 | LO 18 : i SYO $2. 49 970 | 206) ——' 17 ~ + _ > s { Ado oF mn, adit eo hac Gan Orr pepe ete it | DL ghactorclere mn HPS — Yb b. OO aha “yo pte pon bo QRS Ke 75 a Ye dhe. abit, 4. e Pe pt | een. De | R.A. | ( Ak. 300 . 270, 280 << wey | i : : i i : i ! : i i j i i t } : ! ; i : : a i } j i t i : : | a i : ‘ : : i | : : 2 | i 2 t 4 i i : : : : i i : i : : i : i : : : 4 i } : ! ' 5 : i ; : i } : i : - 7 z z 1 : i \ : ; : i i i i ' i ; ' i ; : : i ‘ { | 4 3 i t : ; i { i i | i : ; ' i i : i i ! : ; : : : } } 1 ! t ; 467 bak lee colle, lee Nal to, 7.0 fee gublale dad ic oS Subeti | om. 1 30 : : Ht ferrari flan of Aaa i Wha teh a trot pot at Bat i | Ulex In pouniter CMe oy arts Peg Carbelug deste cdbag hina bog WBS. 503 . (ut) wh ell aceite tern an hncZ fp keP | B | Som 10 om Son 20 mm. US lo ~ ~ *. - t Het - - + ms +b +++ + + tte | eee | tHe | ttt 2 ++ t+ +4+4- ++ +44 [Oey ee ce ferrell raerl mine yrrcbel Lar aed { ? y OATS areal CBr St ee Aree Woon oe ft | Fink wished of spy ——— NG As Cinch 300 dale Dude / heparan er baed £202 And ec / © WaP dala fas H)s o. | | |OMPS 4} | De De Drew 2 VV ! 1 t00 020 | 307%, 289 «| 346 : | 2 200 ©Of/O | 1b3 ast 39.4 | | | Gee Oor| 14> 141 RY | ! 4 le00 ~003 1124 | 132 75,7 | 160 bac nlsep. 020 | 26! WMS a . (ty Wow o7> | 22! deo 209 47.8 I$; SoD , oo3 Ize 119 | 8/0 | o lovo ool C8 | 69 LS ; : ni bbe misio = =§=8=9§ | OD 398 319 | 32 : 2% o> 28! 26? | 37.0 2 ! 2% be > ye Zov | 49.0 vy _OD> | 147 144. 69.4 : ‘ : : { t t : t | | : i i i i : : i i i | : i i bbe > Ca Hechbontle : Steplenl a, V, t op tele , rescusr pol Ur, sy . Mobs. apllate 4. tl BAM oP eargrypre Acbein ott, 34° “~* os yp ~ qel 76 Lactot® alse a — _ Ds t ~f- I. — jae Be ps0 2. Arihe | S|20 3. idac 190 svo q. Ao feos bee 190 Oo Hie sears. Dv | — 177 | 160+6/4 6s. a 170 12D 1.3% 5.4 ‘ xo"? 1.82 | 379 2y¥+/ 1713 33¢ 256 180 343 20d 20? 139 ase bs }o2 S8 359 3/6 To 238 wees: i ATL 30,2. SS.b 24.4 418 1-9 Ko a] 98.0 7a 273 31.6 / 42.0 | £88 : i i | ' Se 5219 sae pus} SOY, es uot 12 Bag, H\4t00 ‘ nt {,300 0/7 1399 | 334 | 27.9 Plake| 29 | Bn | dog | 32.9 3 023 | 22) | aid | 45.7 4 co 2+ 149. | 47 | 68.0 35.1 I Nenad 02S | 300 B80 | tans » O/2—) 240 ABo | 43-5 3 Moo loo? =| 194 Ist] 6S-4 ¥ “ey 749 96 Loy ( Paalaskrh © >/ 330 | 313 | 31.9 > . 2/0 260 2472/| 36.3 > Nhoo | 203 ) 88 186) 53.3 y — | }2] 122) 82.0 1 baci. 028) 23 216 qu - 2 O13 | r0 197 0. a 0 3 coy! }¥o 1>7 | 73.0 Ku, = 71.1 K/ v wo Y-| OF0 083 | tly r ' Blt.) °©?3) YSo- 432! 23.4 | 24, v 0/6_|273+| col 385 | 47 3 ce s /66 162) 61.7 q.8 ¥ Coy qq 89; uA] yys. ' bliwds | 09 334 324 | 30.4 - OsY 2 80 ab 3 s TOS 188 186 $3. ¥ oo 128/ ry | Bo. 8Y/ Zor hh $ $2 apn SO Y7o 160 220 5 Ou , be 10 se. 38Y [60 | plate Kmetlny|yobectood Kab glee 5.1 x10 K tactitet Kyctne (sou) L3-h§ wio7* Aalto 72 las x10 a 5.1 K0> 12x07? we — 1.7 Kio -1.5 \ 4’ Re 17 x/o /0 } i i 4 } i : 4 : i { : : 4 3 : : di 2 uy : i : i \ 1 } i : i : a : i ! 5 3 i 302 LAT 163 226 198 149 00 72D pRyY2r 189 129 44 669 29.49 33.1 44.0 | 66.3 | gL 50.5 | 67.1 | (oo. — , 36.8 4]. $2.94 WS | 1 Ls wi Dovel Yd. Hale Ak athe eam i i t i [10m Ho iter AS ens } i §/° §/ 0 : 7 : i PY ; i : : ¥Y t,t Y78 | HOD hy ra 7 18 P21 doh | 494 )% FS a 8d | 38d BB 30 ¥ }O . TS. Qo3-') 20d 48.| |: 106 ahh gn 3461289 | Qe 230 435 )*° 97 (e838 1 435 (23.0 39) 25.4 | Lv + e 3° ¢ a 607 . 28) 566 % fo 275 . 36.5 Ox 204 | 208 | VE | j:15O) | | | | 25.8 | hot oH (020 Ho | B88 t05 2 noe (0/0 | BBT 389 | eget 30,4 | 3+. 8 myo. YF 27720 7.6 4 40 ~OO3 728 | 131 0 eet . | 7.3 | | j | . a * . £ thin hean ONPG 1 i acca PRR fos oF) Cine Fx Wak Aaa /io) | Melee a an Va é OMPG Hace Rum ay. m der ee . Te ppeticn 2.5 yntra,, . Add avbstat Lak ty. ! 1 ot Nhe. 4 at Jol, ad ty, Tune dD ( o | - | 134f 30, 263 to wh. 134 | | ) & 60 270 | Lo ne (mtn). 3O 1d7 90 277 Yb 1 6a 16 1W) 20 WL. 70 lye ladle! : i /ao 1Y¢G = 12-0 139 /3 293 Ke /62- 146° 167 14 307 : (£0 170 L ! 25 12.7 y 3/4 | 220 )f0 yo 1eY le 330 : 270 190 1 3yY/ 3o0 197 1§ | 357 6 uy #30 20 194) | 369 Go | 22 2y¥y? [v0 | Lv 150 22S 190 | 23/ 2/0 239 2¥0 ys | ! L70 252 | | ! | . | | . jw Crriton | 13.4 a) ular + (0 for . — 13.4 BY puto madd , 1949 Oo 79 bad yr Perl a 672 Akl ar bactle rharratt Yl “3: + fo- Leer plilin, afore | 522. | DK I 22 96 (27 { t | i i | Ib febe . Ihe Ph ae nee vm at vee) age var ume net ee ik Mab “fsx ZS OMS rane. freee 4 Veer 25 ; - ' i 56 gO : nil mL 173 196 ! PHD! Sor 338 i , ; : : ‘ i ‘ i i : i i i ; i : : i : : : i os came ser aes April 17, 1949. Lactobloaate lactase and inhibition ba. from | Link's crnde mater; ial with Sod. Carb 2. ! T4kcing K ifrom Kr > Prepare 4/10 Na | Make up From the Test galactosidas( K-12 Y-53 This Tac _/ Z- M/L0 | tb. of lac} Lba. Grow K-12 an e suspensions, inbculate 180 ml aerated flask for dry, B activity of wash Dy Dr 229 880 229 222 { tobionate' certain] The coils harvested fermen Inhibitid To 3,4 ad MW DOF ony ns. Makejup tubes id M/100 Lba. Dy De 002 251 0 252 010 369 O14 164 g 2s 1.3|x 10, j Y-53 in ReAo 300 008 | y elicits with .Ol/ml 399A J 250 155 d suspensions. 5 ml. ea. overnig QO mins. ' 1 a very a¢tive gal bed glucoge, lactose very very slowl; Ivo be calculated: ONPG ea yo | 64,5) i ht with shaking. rcell prep'n. ; NaP M/50 7.5 TC. | | | ye lactase, M/1000 ONPG, NaP as above. h Vy ~ — vi ke i i i i poate eae to, Po. pctosidasp, but not (vo- vi) (1+ ks i j ' ' i ‘ i i } 4 ; i | } i 7 . Jen) ; La = 44 ¥10 ac 41) (2) |= UT x Jo” ‘rsh le. $234 . 3 | UP foe p AIS 367,13 4/, 23 AB aco 4.5 x07 PF Enos me hb Aw o t . per ee 2. . en ae t is t : 2 a ae . . ee teen ~ 0) ! re Qeresere Sasetesssss x yy | | 2 peed ee ee a a eet ene - Mas ®OR SY | | RmAwHeEH AS So < ! A So---A AQW = = — ~ ~~ . ~ = pene - ov ne wenn nme ances ne enone oles A \ Bp VQVo7To%s~ & 2 (eA gah nacre 0 & - 4 6 oe rer 2 = Se = 7 semester ont or os a Se ee ee | dD Dt (| Mie | Y2tae o7o- 399 2. " Qly ogo 087 3 |@ir toes Wyte 120 790 (Tram. yi" M/, 99 324 W) Y2 Lae a2 170 | 2 te ble Go 193 | P iy’ \Laeanf go ofS B we /, 4 /oG 1 | Bucy 4 , WG 3/0 "0 "a /iteof | ‘110 228 A=K-2 |: lec [oo 2= 44 |seo : 3- . 4 /svo Di D™ | . A ps AL 70 2 jNe 3 re teva — dl yh 4 1 Us q0 mE ge i { { B: Y70 c > aR 73, «(281 (109222 $3 470 (387 us 140 | 028 Ho | 120 — eto WR | 1B, bY 41 7? 036 Wa =|? | os 89, 3H 150 o2/ 00F os7 OYo o73 17! A “last lowes, - /EML iY. (Yo fae ~p) fn ee | : Yio (and crac) 5 | | Cait ‘ 529 po animal yo, be fs Pagal wo owl te Yo ) RA Dovel - boo ¢ {ye 200 (800) qSO | , 590 0 72 O18 | (p43 080° 322 O27 - ay1 ag 52% Yio lac. Oi, bar, These hile cart he An ye 380 o14 prise 3 h ney _ 7s UW thet s : 20 n9299|\ roto Yo : decor R-A. Ylou bacn) 048 ISE& 94 Aco tr 49 076 174 9q! NG {[oug ” 0x3 TC} S2 o48 Us 663 070 oO 00O yo o47 O56 of 0o2 WHA, 072— 0% o8 on 2 | /99 "9 03! 607] 3 17 Fs 43) o4vy Made 013 vo 4 108 460 | 350 324 2. Wl S7o 452. 380 1 07 fff oe 33) . 1Z0 © 150 02.0 ols” ©o80 086 oD | Wr / 09G Ng 020 o2! 2! ee? 170 o/4 ore 3 262 139 by | . | 35 I-22 344] 6 sae 7 hs me 22 18 { i i i | é/xX inen« a Ab! — LA. 13m. SF ay 324 Y3q | gos. 607.2 05.5 0073 [dates ucg A> sv) hyo) _ _ Naweat ¥00 B bee. early rere’ | ¥2 Slee s halaerr Sept rend & Tle | Molae , 2pek H>0, real [spa Haber Yuk sajnetis| te |M/ro ogecle by/sodoffn fem bof y/rem : De | De Len. RA. VON |T=0 oY =§=| 497 a4 YSy,.| JO! | [00 7 3° || $0 wm. 0b | 097 gs (7047 | 079 1090 ' L 7 anes es . i Tink [oboe Amt SV Pa IR fan oe ta. RO /OX., iO. | hal Sy ea bak Wah WS 7S, Nok A). Wo revit 3 ORS corti, O21. B). peptre- Ink 2% OPA TO Di ; Dy Alcor} R-A : D: Po, AO | BA IS. ve | ob) 07] | -00s | ObY | o8f | Oe | ofa S™= | oS&6 | O77 14 0051009 067 «| one | 235 os] 72 | 048 | 078 (+ 034) o7: 083 | 30 | 2M | 2k) | 82 ast 019 | 180 | 670 6 Fo [Mewerh x12 Y2 Lees, BL Oy | | [6D firxple |e Meh dees tt Hp. x zt | ie na 2 bee ! : | Vrakatle le lek Yen [vn piphemrnde A. “- Polfooh ! JOO AN. Bab ho C. ab ene : ee (foil one. 2.25 10° ”») D. ab ghnees ra : , pp + ghucese. os "le agite u/s Ha brffer a le so54 De : | De. ! Aue RA. A. 050 | 1 | ! Pp . 650 | er €C : D py ff. an 1d beac Yo actloaln Mook bff ! i ltd te pit } | 060 J. by we t}yo . pMns- O54 730 O6f 104 o56 | 57o /60 084. 274 — { | t i Hoxage fe ragelaclonshay S78 mantle ree ote : Yo he. : «) waiter b)WaP Nfs QH7s | HP de. acess cen Is gos whe wo neyell { i i | oS 158. ! - Of | >750 [Semel Wy ofyo >75O Comms. , 354 410 : 3 / of 375 pe tap snob ibi Hagan 7H 544 : is t by JOS ml tack. 430 (Sn ~frt anda DB, ~/ Al, A plea ruittg A, Adah. > ark 2 sn eae Oe Prada Mth thefts KN gern he Agen Drdvte |! nth antler, bifpn, cb, 1 | | i | : i i : a t i | | i i } i a 16D a/[s tk. th ephy « M,, Ca, | Nhio BF Pryek therenset i eaton) " nf 3 | a 024 1 cM | | Ddedecele Memulel aud | Piok antinhatcr, ., Ahoy Yl tte, tele Hennes Mord ben Life Pb. fi awh) 2 akeee | _ a — | bow | the | | "tohecim tae Oe pak Ma P yf. WL RIL icf 6 SOn£. lh ord), ~ *40y. rece L ex|, /ng.) Ta [ ig, ane hud lols be “feutbira dtinwk DM there tilkisd atin ualo. 6 o>. Y) eee Zo A, anel 8 the}: 1/70 = ,Olik De Piretame Wom, R.A | A 087 1193 H 8 ego |3aqeen 2S7x'J= 868 W692 meq | ~ hye A- sad 22. Jos peafavonions| conga cacsying De. 067 of8 530 47% 7s hare. Od nl tf Jk. Tote! A | 3280 B 24800 De T -O Srp. 2080 BO Sra. 146Y yn3) haa Aved 4 sheared ach; urtt 6 x a/n /o4 1476 isT Linke, Bsr |= Beer ke Je A BIAD. 4 IsT afi es + WS. EO he tape OD he 29 YW... ao) YD j. Calg 3160 5 f adrrAd cells fs Lng Ped WH4.$ als foo dP. asaitd 37° Petal 1°. L nef ink), are poke ok pt e. 0 | B)+iSmk Mab Y/s ph7s. DO 2oO ! oe daa te toh hey. | Del & ; ; aS = ason for asipays. ( : | : Jo, Ai is) atl one! Cte) buen Be ater ip ele | | i Wad. reble & WPS ae dele. ones pi by. 4s. Na. 8.5 wd a | | | neg dk : | 2” 20 s 02% ! Gos O3o 190 oss 20 | 2¥e 034 La SM Oyo 6-4 | [oA ! | ? : | 4 | | 40 : 54 13, os 2. | 2 OF 7 22 apm, 27 wee lop ate: 30 Ha 173; 38 1 | B°%, 50 154 ! | | bO5~ Jo ws Ninehes of actiation Mo P beffer- NTM 304°, Actwehn hiho: 45 a = sown tenet 35 se ummacorm ss Hy nubeoo —> perenne in neem net ye Yo cee ne ethernet meta gteessatn mpm} 30 boSQ Le - aa , $195 +70 koe Yoo Roy kf bid? say P tha efi in ¥+7 em , YO? T/2 a2 0H Y ccLs. bere 72. +7 a73ie3 ae ae ys) YoS +/0 i ‘ 60S 45 $0 15 eee eee cue gee sane i peer geen den ng ete hee eer cee a ee eee ues Yio ee wis po pve inrnserceie nde meena nem od 60g yf “T JAA ee — 9-00. = es coy Ui \ - | LA tens 99! \ Wash owr: ”) ‘SN ~ SAS 3 a PUSS OSS PS yi PoFhe \ Wurst And wopa om 2RDD Agee eye f > rv ATs oe oO =~ er en ee | t > T~WM | MMg TAM 4 -] Q YX Og agvexoad 2g Q rr ~y . A No MST —' Mm © ~AxyTw hE a =~“ TT A2or ~Q% ~ Q%® \. on Tre EM gD AS TOP > o~ i ° SLs=8To =F | &K | c TO Qe e (YY Vv) —— o: Ve ah ov 0 | We es 3 Co A ol & i m pv mM \ eee. glucose txnxw maltose coed 1somaltese, bo aaD [s@ 3 <= Ss cevinalle 20 G a og 7 . ow : 9 & “ ® . o “yo dae mee fees cup eee veut ens i sala trance nt ih een te om . t eee eee ee ese ee > _— /o 2O 30 |= 2 22 pe we yw ve 3 42 ce 4 lod Sore. auchyernoa, 05 - on (04° Aenet tints cafed Ila oO. i yo 80°F aleali per). © Jo 25 30 35 @ { 22 Oo | ye — joel pes 7. 7° r'3 a $) 27+ “| 178 [= pos 37° 3l7 opti 8,197 oo ta THe |] Tle ie (IL 4 , 1374 H 2O 3 a | 33 70 43 2 ei 5 ef 37 73 «5, x Tx 1 | Test ils 5s fulalee [elt Wi upid at fest 13 w 202 “3 Bu i : . x i Kid prem Ve, oA 41 JOA Tan 18>) 19° /6-2 215 }7-% DVI+e 22H 19 0 19 ta | 25 7 2+h ) Sy 4 ? 38 zo YE +] S/ 3% 1% st S131 7956 70 43 Ist bh qs 28 | x“ | we YD Te hohe % n> Ny, If oe. {OG 43 Wo 24 3] a pede sppa-€; Se 46 SB ly jot! ISS +2 /60 +4 167 - 607 ods (r) re Aachen (2) mati, gsr, Hob, vidual | donk om ee 10%, co Mert los ly Joa Wah “f 100 St 32° (15, 607 162 78 nr? 127° ye 1v )7 poe Vee 23° = Green| prettene i ' F r fe saves er ian 1Q Thimne _ mr Mattosey , | 2? Of JO df a 16 neo qi5 | +O o6. 69 . Th Sel, IY in ut- ¥ fier 47° | 20 09 L M9 >" q be 38 +8 29 +|20 Vl 4 147° \ O 9 90 sy + ys Ys +13 22. 0 jSO 1] Hl 9 79 +46 yt ag 2/ © MP +41 i phar - q¥é 1] + 6 G/ +18 zB 9 Ist -v qi HWY +268 Wwe +27 | 2S 4 So +4 9% 37 + WMo+%k! 27 2 ed | oo ee oe [3 HS FOr a ppoe 60 +2 wey ee yor Gt +t )> Wun. 44 48 }04 oy hour 26 Mraate cfclrtins J goleclre rene 1949 KAsH M04 “fafa! | 1 9A 47 U7 +1 181133 a 1 N]so r- 7A Wd 'l o 495 417-78 58 yo 7568 £4 Vi eg, Pylecah | i fin eb “a to, 3 ) m n)jeo iy fe 3 YhH 24 | 30 , atl a DLO | rr $34 8 HI ape | 2 Mbt 30 GC} yF 83 $3, 74 61 10576) 6 a4 114 q] mre XX Wal heoe / to, IST : ISO 41, | yout! S/o. | 10 +i 149 re ws ol. | Bp — l ‘ 4sd 2” 320 IB 4 OG OY 43 " fy Br FO or lor 37. | } 4 é _. i i i i 96 | LO 52-1 | /d7 meee 3) 150 16/ MW 216 206 2y. b 102 I 1P7 Nn 2¥3 rey 1 fy 4 7 | Sth oj | y ; [bp . 4 T } . Q | j h i ; Wels nd reclteke | } i i | ca 307 3 i i i i t “ i t { q yn! oug ee =r a bella Wak. MSs St gee Matt tos tho. Wak Yowe, a lb "ome ys “Tz c aenetinn Hens oa | » dati oo f 4 | oop | hae i of 4% : : ' ea peg elo ! i 4 ' ; TT i | i ; ! nye 2 18 -3 16 47 | | 2S ed Pah ID ; ! | pvt ps 26 aed 27 if ! : | 12-8 10 BD B IST 7 by 28 O8 2s dS |° of BO Jo (20 l¥g | 32 Pr 28 52 7 p27 Oxi) ys 4 41° 189 42 35 156 pse [6b g S/ 27 pe i f ‘ ; : ! i i i : i | i i i ! i i i i i - i i i | : ; : : ‘ } i ! ; 3 : : 5 } 4 i ; 4 4 : ‘ 2 ; é i \ | i i i i i i { i t i i Or = cA. 63 0° Vote ho n3?”| Dyed nie yep a . / QDI | 4.88 Bi 19-57 | 1.74 ¥ | 2014 | [,80 5) 14-97 | 1,78 6| (8.20 | jez A 7 | 6.493 | 1464 &'| 1899 169 9 17.02.) 1.64 jo} (8494 | }. ed ni 60 1n7 1-} 16.86 | 1.68 134) 19.61 174 4) 78.26 | 163 | 14,8) I. 74 1-82 2 14- 7? 3) wW4S | 3A. ‘ at IL 37 19.835 | 3,77 6 18.95 | 169 7| 2047 | 4,82. Pl 19.67 | 175: % | 18.86 1.68 (Ol (9.6% | 175 Ti 1401 1,70 ! o gs Nr | Bed : 6/} } od Kid | Sapte 61) - Splenda, l¢ 1949 ~ _ 2 _ | Ws | \ BH x y e y S Pi IS Ie y sig Noy NN V lon 1A sa ¥ TA s 2A |S sat? 12a |8 HAL T | te eo | We wel (2399) Cs) sD 19 yt 2) por? 17 32 is3 is | WS I y —P LAT LAC |LAC LAC = par GLUE PEP 50 | go 260] 37422 Ol¥o 3 57-1 | York [33-8 |b 61 gee ZOIk 361L]/9 10)36 IL 5045 [4rd 2048 6! /6f sie 3S 1b) 42481 2B joi pr 6 [6554 |2Yt4 ey /6f git B36) 43 4l23a 0 4315 js |eoaqirzy dies | ys! \, Tae 174 1$020 || /TF (332) 24] 13 24h 199071 3-31 74 /6/ 8 73" 176 ISA ze Wl 6d wbl217 Bol ssf) | 273 33-S i760 wes ch | gk . bachre fee P, tore. 1% Zz nv, 7 lace | be antes nL K\ ghren | calles weg 3) bactre | Sle | NgS!y Meo oo 4) Lactse | fle Asay + MySoy u|\e. (f Si becteer te of — ' ¢.| Pee, : i — | flu : 3; — Faglent 4h 74? 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