) . - : . ALLA Lo_ lun BAD Ort - PALA . 3 ne We aes ip @) lacy - wae thal im ee peng (2) bacy ~ t). arvdeck “le @ G tha ie bac A Aa haw tree le bemal kG, Cut Cy bet fe fo t ~- c an ¢ (3 ” Lae . G AM ve fo peedcecek ua t Plus sk Cops Le Ae ¢ barneseee ahtine ke, o)- ae GUL f a titara POM TR CH) C hee [e wyitrr bear of iy-b OC egae. wW-67 and 164 ow | fred (1 W -10 & ¥ Yuo. Cpe WG LW Jo8 >» here oral Le ety Mata lee Ca tl: uf 3 v) W-b7 x YG. Baal, are ovearamd) + elma . None om > ylecree TH+ teetaf fee ,%. ] What Liralrage aebtnolge> au decal, ‘( the Lacy- fe eseerey « cot accord 2? Thetwmderuatinrr au BY Lact vB Te. % Lac-v,>. beey aay ae dr cle lalacdte I, ex atuabilan Hats tpl Biche ge 9 code yo a ae tU,7 tanh ins , C5. bn bee vi beed ry _ + S$ ~ + + = R + = “ Voy —_ + - a, . ae Rk (FR Yer, — Js t t _ 4 L + ae om amen ac % t 1 , +5. NAA PM bee Lacy V Th A. = aS a + TE ~ t 7 oF AS +R Ly ~K naw. a ~ > 203 Cevamins — can Thaw /4 o&. al r CHO le seeets ¢ TL,BN LL betes Aine +f, . C. lL. Ma seeenute [Ve ne io 5% ( ju) fepadtenne i ; (coat > F/ 3. 1% Duqnale EMA A " 3%, FV. arpa 1°f, é " ” (5% F. va 1%o ¥ genes iT % b>. Ciupta W-ioe~ YH wm 0 fA wel faces S JP ay. [ w-t08 42 te | Lee +... calior caret be terenicany 6). Saks nf ma plete tah Pacis L. . 203 3P. 5” | [+ 2. No, pesteArags cotoece's, fesepnaed Aacbgercnaal Cases.a Vso.) 3.+u Mameseras pecctaplis > [ven Lira , haaey le Face 1r-, Ye LOE Mag thane 3) KF rencectoon 5. Praponits 6. hh 5 1 Porpond nd gd B57. S:30F . Hr, 18 proper dmlaorensl. 3,4. (aapesoais) 3: v url decloped colons, sapserall, Lac +, Mens ~ UH: de. leer anne eceerturtid toe ~ posaclly algll 5,6 (aspect S; Faster codemen, Lec oni 6. Dekh. 9 Ai 2. r, 78. Looe pert , ay. | 3 aderers alll, pee 4 Hin Yara pecnacts Lear eas y. 5. (ull aboot (x 3. Lac ~ Hk b be rll, thurs beet, buf wet v1 ‘” : 2 LEMS 0S m0 t EMS- 1b3a - IS %, '¢ Vas An lan EMA’: -_ Ul xWys O Y vy (OF xw (2) w bat 3. Lely + "f th tobe coy Z2os (3° wW-61% vue Menpatll (1 ettmig Yptetec.) S 4, Mo - - W-67% YbY, ald ssc heglern os wi W-67x Yb Cham WOT Y64. se - | ead & re). cake, Th ppt pee (Dp: Z2-— eK. Mo +. bac, , 4s” ‘ ja W198 R (D. wt v Vo bat Lach, @. wast vy (HEE Mel B. G) w-I4S x eT. @D weds + wos (SD w-237 x Y8T. (oO. W-337 x WHS. bee 6, Adi: ; _ ; } 9 3: | 3 23 2. a5" { o7 1 8 0 ° 2 le 2 o oO O Me (o 3 phakic ). Lo oO ° O ° 7 _ 2 425 Iv O 5 3 C 6 3/22 6o [87 265- § rs — . ~J ~ol <* t 2 09a, 202. Wwe6t x ¥46 () (2) W-133x Yoo. Lu-67 x wd-)o3 Brn fren : + —_— 4 mw L4 ro 3] 4x” |e. Ly e104 > ,D! & /6 } 3 | J 7 : , > he O O / 2- oO 2 07. aL Nutritional mutant run: 5. typhi-murium 208 May 29, 1948. Irradiate suspensions of S-20, and 21 as follows. _ Grown (6 h.) suspensions of S--- in YZ-glucose, shaken, resuspended in H,0. 8-20 exposed tomHanovia output at aperture of lamp in quartz flask shaken by hand. > mL. suspension added, .5 ml removed at stated intervals to 10 ml. tubes of YZ glu cose shaken at 37. 5-21 exposed in 1 ml. lots in 10 cm Petri Plates, exposed at table level ( ca 12 cm) 5 ml samples removed from each plate. vd =— S-20: 16, 30, 60, 120 and 18 secs. 3 , JO4DEC+HIF GOH Fey, S-21 2, 5, 10, 20, 30 and 60 secs. eopetes L~/0 typ PO -bo HER, DAK 8-20 JOseemrd anh S21 SAcenk cypetus 10>" arruf pale ion rreameriaf Manes cna , +P 30. Er fries ) 9720 = ot Sw —{ avl S~9f > $w-2. . Ca. 30 patio carts end 10,000 cxlonres. phd , 9 gear atp ty Atrur SRA) I pee 32 (eh Gere S-20, Vinten P~ 2) are 3-0, 22U~ 30 ace S729. Nufyuts 2-0 -eL sw-4 ( ) fu S-20 sw-5-8 ( ) foe So 21. _ Lac “at, fle) He Vits Y€y. EMA ENB. } tr 2 te 3 tt um t+ | > He . 6 +t . 7 +e x b & tt . 5 } 49 +t . Vey) 0 —- + +h — + + £4] y Sw -3 " ~ tes ay po 4 ‘3 SJ 1 3 } iC Woods 16 2 y 7 t7 " i , as! if - ++ —-— t+ y v S sw-¢d % 3 re uu — a ah _- « _ _— ee S sw Ss 43 LY ~ _ ne rhe Sto -6 9.0. wa glucans Ww -— ++ - + s0:7 ay % +7 2 3 — - te! - te sw .8 woth Wie — : : cS CARE oO me “ah Olorers Prnctareye hac cee, (Doel. HEED * veo age C. He a vay. Aye . i 2-3 - > aaa £ oe ae t det t 4 + = + + bEE + + tte 1 - — t+ — — —_ _ YEO Pv 8 — — — —s¥EF — — — +h poy S- 4 YA Yi Gyth . Milrax Pura ee H-C-4 Ur ts. O ; 5 ~ tH + ~ _ | Hoyt, é. ~ th + 7 ++ VAs. fale y: on?I. at sit rH + +++ +h nC 6. /Ovrrs, K Vi 47: 1091. 204 | Cob: ) Vale afte fw-10P and +) a lee CHR SLAB. cdots and) phiake ot Sour. Teck heal? ard w-1o8 2, 7/ audarn TS : - a fle K,R faa / A R bu - FF Vi and He DR RS Vd. SoH ranalg y y k R é R S , [08 S $ f Vie (2/2). / Ste + Ble. W-VOD~<> Ts *. (209) R. barry, 4, and) |/ V. Crp rer . V0 } Man ap 1498. a | Ce, la + kl EMA . ) 58-11 x W379 &) 8-16 x Wad V wet « V4 3, . | i Coles Seed. Pick tolonue 0X pareclnay fy tat é Lee, Ti. 3. Ua mpelf (lect | ). | 1: Powe? , prabelly vet fase. Claas deagt J Lae bars Comfrrnal Shale © Hallort, Hm AYSS ese ME~ with bears + Eretmnelen 5. Wald oR. > eralucere fbanm Aatoae. Prion forth Podirecn a7 bee on ~, 6. Tandems pest shitang * Wel rm a E.pdee! Pick frareelrm h tacfore. 9. | plate . /+ , Beet — telpens. 2/3. (y. Lebnowd telrrur . 6 | 4 t fee ge fll Eugaumece "$m" : 8 39 w “ vo 87 | S18 Ce BH mepamendig lh Tent "+" and —" aupasitely m EMA-bac b,, _ —W +R —> +S. y XT Oo o oO oo 54 Miele fone ge FMA Gb thee EAA. 20 ~ 54 {I-¥). Nucteckerr, 4 Ww -67. 2) eg 2S VE Tea mA: (.Lt+ 64) Pry -Ars. (. T (a) + LY prcemk uu "4 Vee, Ob. . " N2Gaw &) Vits- TC) glucess- atpacngaine . a ay Vk, (1. U2 Gare. 9) Vits. A B Ww-6b7 5 8 -/6). ] = t+ > +r. 2 + te 3 ter ti i ete +4 1 ++ Ett 13 ++ t++ y _ _ pe. t+ were Pree Ar? W)-6'7 42 wet muleteomebl, durtinguerdalte from SPI. Shuck at on Lach +IDN zk. P26. - por eh. eur A. Lec ENB. Wh. tr oy. 19. +44 +++ tHe lecy— Mould be perdatedl wLbeus pqs rn fat A ptetor 2. pebllec tecarcbertin,” 2/2 Tae 30, 1498. beadele + shades bs 1 —fcerre fecbeo, roemugtel fs lb, bs ltlx and us = Ast. Tlo) plater ot tn 4 Y2 wth Sunt werk ~~ fas pile sereptcaince fron bres kcal AY b 0, 0 Oo, | CW 1Q) (ure repacatcls ) 4, 4 C bw LED Ny ehiaa) 1, 2/3 5 fee VHS. 2 opriodl bunk Aud prion Afi m VAueA4A pee corhaurrs Me “/ink of Lee mee | ). (6s 1 Canin) © Ps Ube ( PS ea 10 tn tush; ars tamed” PIO SF hilt truer . Sp Mercoet coflstad tahagernnd, ut, a OY cee 200 he tut tothe , ta babagin $16. Ca 100 hecliud cele» oe A. HHeaary Gert . Oo. Pl. g& se dectuct, 7 a tot vio c vee ). P>- 2 color? , > alls An. +8 PIO. = No eotoneed. S1 fee WD. oS. (pate uwitlic. hurd) G. Soo wlrus (rned ?), S10. Seeeal beceecbied egtarccs | VA Hlewary een. Kup Heglived (AES fox pouife coin on 1 PE ey col nin 10° § ancl UA. Sfecebe ou. Fe oigh br a 10; o Ib f ps0 ne - . brlueclnr 1b J S/O +t ttt _ ttt + wae “ae ; et] P10 +4-¥€ - ~ ey /S/0 0 tA - tttt+ 2/34 aaa tC a a 5/0 b other; cect teen hea AS. PS P/O a P/82 S/o (959 3/8 S$ Soe. Stel, suslovl ¢ ee war uel. I [S10 da cal ile seh cm (‘atin Jor jreabliicd loa bx] 10 ISD tro BO |-200 rE 49 oO O z . — Ty ($10. rues i SED 2 yp [mrrcrtl Srouesel yo 6 dunt e Curall). ye Taek Lhe follows, aa raudeealed S5a0 S/00 PP jo P 100 Sio 16 py si h 6 910 | / by 2/0 Crm py. L£/.3/3 " n fe hw Ploo 5/0 a (er ee ° vee 16 5/0 R "Plo Ss S fs “1e¥ S/o S S R § S “oepio' § S 5 § S ibsi0/sSo0} & S RR RS INsi/sioe SS R R RS ks Pim > S S S $ S [GY pfoo S S SS S iso-prre* S S RR? Ss 164.800 -Proa S S R $ S Stphrayin ete OK, hrtuctr blboiur Ha 10 reel 582 nmch Levels. “ot Mo prucecllin, reccetants so foc vetd Dtrabeod , cr WA, fe rlhuers 2138-1 arch 2/9B-2. vy poe >, 1978. GY w-aat x W-4S- (D). weivs x Y¥0 (3) w-inex Yoo. Serb arsewoley ) Aik at W-YS arb VO 1, bac A +(B+4). Pd. weySt Y00 067 well gema mn Be 7 taciobassen AK meme d the cco Be ahs cory eg PS: Uo concede lech r?,. 2Q. No eclerntre a, bacdA. Srna palin 7 () have colonia aigelichy reattoad 3. Wo eclmres or TUK,) or bee At B,! FPG. 1 Wo Color . 2. frcke ealmis fam TI.) G bee TH. 2. (OF elon om eke ) tere 4 14d § i -133 vy. Ar 4) T{,) B) Lae f-(o) c) Loc A (B) D) Lae ACB, ) PO. Crbovies nor D aferme Ca 6 a, A F "ween few pee A. cab] B. A+ v pbtes Cc. Ce, 100] pte Is] +: Heasny beabegirned 39+: 5) - D. Co SO) the A. aan + fest Tl on ler ENB. “Pahyuc tbe bany Bp. cat D. ea, + aud — R Ss ¢. + 2y¥ / + oe ~ 1@ 6 —— ee 45. D. + ] t MT ° = yi ( Salmonella Irradiation - ANG double matants., Copan June 4, 1948, Irradiate washed 8 h. suppensions off SW~3, SW-7, SW-8 and S-21, in 1 ml. lots in open Petri plates, Recover } ml samples to NZ-glucose broth, and shake overnight. In 5-21 series, plate .05 ml sample from the initially inoculated cultures to estimate killing rate. 5,10, 20, and 30 seconds under Hanovia lamp. Marsucung merlin { TK AX Px go aS 510% He hbo Cam hy extirrald. ~ >. p $. f - — cq . 3 \ — . S ALC: > OCD 10 234 43 suttrterd). 1% g 6 do, /a. 6. . {Qnec4, , herchirle the br crasbil, Auspirceine? , reberrt, delecle as udealy and plat iM, rity pelitlion plalir Sls Accapineelere a nrbacactetle — St] JOPG 36h. Cou é Wlare-Jeutigtad - Gar — 5W7 BOT sanneie alg epee Lost cur. = SWF. Hak mT) plea aunighe Lager J 5003,-7, 4-8 woendecnliy 3,2 P7 cm.3O0. 7 aAt 0 (+ wife) 0. 8 oO. ©, A tach. e fapigblie, 3 27 Numer /, tgegbecsts SP a, l . ry : es ? harele oh lord ealpyrce? - LIP. 216 — SW 7 aerers prralf rolmcis mhy my perme J ThirvnT pol b-ie-v ag plenend Pap topbane . Moat wae — 2m aval haber; fhe follrirnss re F | St: 19,29, 39, 59,79, 9), 94,60. it a 4 "6 > J eo eee sod, (dole acorns )- Tet S21 1-3 eA Sw HI-2 a 1TH) Magy fbr. A +44. 7: Niki Sw-3 4800? ny WA. in 0" delutons weutd/. Sw-® Sw-T Ne erlenst eo eT em) | TET fet Qala parle th plan | fens 11440 SO — G. (pal. ) 0 Vits. palr. HS pala Ne pob He, PP. fare ~ + + - tt + Su -T (lease, week t vel) . d S$: Corbet. O He L IL V L: ‘db dL: uv Ievobdbey “To. ter = - +. ++ Ltucane ~ ceabeusvia - vale +r (yf -Z) +++ Het tte +H tee te +44 “eg. Hp su «8. Chypt.) oO Pap. ndete anttaan. nicotine 184. - tte + = 7 pat H+ Cal. i Y¥S3, YYO+ WOEF vy, Y2.. fons ce | Atop zoek on lech +, cena + 8, TUB, ). ARUN at, pul, Cos lL YS3y v0 fore, 7. V108« Yeo | [ea Tan A ( Ale. IA. 27,2334 oo TO). Q- 14) 0. n, ReA. 7,4, 4 fog tolosia on ba S. LB. /,0 ~ T(n,). 7/00 , api, lee A. lo+,22—-, IS BY, tae 8. (pute e Atle bat ‘12,7 om bad A hel — W,/ wy Lar A _ PA 62- A+ bac fl. ) A ( + /- — erupt G1- 6t Lath. bali nS. 30 - I+ lac S. Us, | re ~~ aa pil. /P. (LeeS,) Pf. 34+ >: glo E Too ean +f Vithalen J S- ww 7. 220 Te oul Tlo) att: 180. 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Peabs rl nr eek pol: K = fW-/L- 2 = Bb. ttn /6 hhh lef v1 cack pele . QWs: SHo-7 W: W-Yoo ( Lees -). A. K: Conair trlorteas ov far pra ge bh oetey (2 . oe 23-2 a0 hell) colote dah ud SW dls whale donee an) Ue aleclear gore . c 060ON crlerlers ; MEE celprees “od W 4 SW) lyre wef dD. an el C. K went feacke . Dod torentat,., boll, mm 223-1 ¢€-2 ws, heroes bps - o¥ las - LAG | be Aer — L 425 Get (ptath oy oso) OC! arr: wg ® Nuttin nee T. ( (48. su -> y Cy. aie | algaeccier ely 1s tee — LL [nh + a 3 supy + hfivsrelw). - Y jovy - ~ y. 20r ~ Sw -1. Valwre hae . ; 2. 2, 4, Ss. 6, =. i 9 Zr, Slrinalle plage, por 14,496. ¢ Pen cree, Cuttinte Sw Crilufuss Madetoy Stwoye A ( Sauonye FBTR) Add Soe 4 Sub $20 SDI Bourk het, . Pret 6- Che PE oe» tanh eablan (12F-L0 is) : A Mn ale (O tdaag ‘phe "reg . day dct TOL we (gig “t ho fcatt tata} = yell pligpa, @ fot ee nereuh Coin. VSF- Al 5 cnt gen plop rite ak vat wo aad pteiels nbn, roricloggres dmKnal are rage Aecrpruncrion birth ba pod Pie fom mle pps far i Tlcae aug vast he pe pire 220 * Ww os. 3 rf fualh ©