INDEX to BACTERIAL GENETICS ¥V. 1-3 Yale 1946-1947. AGAR, purity 163a se R version? 426-429, 43304 508 Biptoidgn: 4902 408,880) ALY, abt eho BIOCHEMICAL CHARACTERIZATION, By mutants. Y3 160,164 Y6 160,164,1698,173 eee Q I He teh Y38 218 Y¥39 218 Y44 289 Y24 oystine growth response 211 142-36 & 142-57 DROPPED 164-5. By substance methiohine 164,165, 211,289 pab 26 5 , 289 a-ketoglutaric on 679-662 476,479» ADAPTATION (non-genetio§s €79-662 to proline 196 nutritional 348 AUXAHOGRAPHY 163a, 168, 174, 178, 184, 197 DETECTION METHODS controls & viability gio? 183 Trials 172, 548 See Salmonella , 197, 194 Bacterial Hydrolysate 198, 201 Small colony vartiants 292 Permentation 332, 333. GROWLH ACTIVE SUBSTANCES Biotin in soli 206 Inositol, a. 206 pad in cold 206 accumulation in coli 209 Fluorophenylalanine 182, 213, 214, 215 Valine- activity on L£1§ 173 Densitometry calibration and baoterial production 185, 206 HYDROGENLYASE 470, 478, 479, 485 LINKAGE SUMMARIES Reverse Crosses 390, 313, 315, 386 and see Crosses Butritional factors 363, 364, 4528 Map 371 By 370 Cla 509a Lac V6 918, 528 rin” he Ya 527, 537. MUTANTS spontaneous 175, 194 DV resistance 191 in B/r 199,263,273,274 ological 269,535 Lac revertability 383 MUTATION RUNS 167, 172, 186, 192, 197, 199, 221, 221, 222, 225(pS terminology), 226, 263, 298, 294, 335,395(Lac-), 546(Lac-), 366 (Gal-), 403,463 (FERM), 548 (C1). Reversion Testa Y3 161 y10 164 BB 168 SERIESof: 170. Y4l 277 Y43 «277 Y1O 308, 339 ¥53 365,389,418, 427 &UV 241,244,247 I-15( shift) 307 B/1 538 RESISTANCE MUTATIONS Cu,Hg 180,428, 430 Colicin 266 Proflavine 382 Streptothricin 443 Crystal Violet 362 Brilliant Green 428,430, 436,512 Streptomycin 419, 428, 430, 426 fyrooldin 428 Malachite Green 430, 436 Azide 430 Triiodobenzoic 430 Spergon 430 Other organisms than colis:: 465 Haloacetate & Acetate metabolism: 466, 4685473, 474, 5,7,9a,480,1,9,497, 500, 504, 542( quant). PHAGE STOOKS AND MUTANTS: Tl: 225,228,230,235 T2-T7 295,507,513, 542 C: 29% 240 Staining of debris of lysis on EMB: 501 Mucoid resistant typea 457,516 Resistance patterns: 354-7, 360,506,498,511,517,518,520, 521 SYNTROPHISM: 195a,197,179,181,187 ,193,1985, 204a, 205, 229 ( priming) ,842( filtrate analysis. SUBSTRATE UTMLEZATION EFFICIENCY: 202, 234% 203 GROSSES: JI Y40 Y41: 252, 257 ¥54: 349ng 679-183; 252,257,313 ¥53: 345(laotose), 353,358, 361, 364 Y41 BE-161: 252,257 , 260, 287, 313 Ai] /3" 319 Y24 3 259,278,276, 287,290,296 B/r mats. 277,282, 287 5391 Proteus: 277,283 Y24 BTG-183 252 Y9 264,270 Y10 276,284,285, 287 ‘Y46t 293,297, 300,318, 321, 301 HS t 300,304, 318, 301 679-183: 301.313 679-680: 301 ¥53 X Y6l + 353 Three Way: BeL-T-: 326, 338 Y¥53-Y46-58.161 353 CROSSES II x Y1Q_ Y87 494 Y1LO3 539 Y 61, 364, 370, 375(4n BTL), 377, 415, 432, 445, 446, 73% ew 723° eal selection eff. ), 459(par. ratio eff.), 472, AAT @lection | Y64: 490 Y65? 459 | Tt Y¥89: 471,482,486, 509a Y94: 518,519,528 9 47 48 4 » 509 Y95: 518a,519 Y96: 532 Y¥99: 527 & Y53 6: 52 oe znotes to Treffers 58-6315 376 Y68: 379 , 387, 439 Y78: 441 Y90: 502 Y91L: 496,509a Y92: 502 X Y64 58-prol: 417 Y26 : 526,534 YET Y26¢° 523,526 38-161 Y67: 387 Y100: 537 Y65* 404 Misct MUCOIDS 414,467, 488,503 y8O x Y81: 440,451/a, 461 Prol: alleAism 424 58-prol x 679-662 416 Iel5 oatants 425, 431. grWay t Y46~Y53~-58.161: 3568 ’ 398 » 412 B-]-T- 370 Misc. 554 SEX: PRELIMINARY lst Expt. 224 (Jane 2, 1946.) Prototrophs and isolation 227,231,240 Stability (detection methoa$ 257 Controls and repeat: 233ng,236,239,243 Mutation in prototrophs: 238,256 Recessive recombinants: 245,248,250, 254,255, 272 Phage analysis: 258 Control on cpinoidental reversion: 268 SEX: TECHNIQUES: Cross~streak on agar 262ng,271 (See aberrations) various liquid media: 275,280 Yeast Beef Broth 287 Bacterial Hydrolysate 288 Peniciliin (sygospores?) 302,324, Packing in centrig@fuge 321 Growth separately-conditions: 321,326,340, 344. ,3551,552,358 Kinetics 320, 384,399,438 UV-killing effect 320 Chloral hydrate and swarming 328 Synthetic medium 369 Storage of cells 392 Tween 80 402 DRNase 420, 423 Use of drugs 442,509,514 Plating media 449,493 Colony selection 464 Phage 519 Agar hardness 529, TRABPORMATION EXPERIMENTS: K-32t1:: Bxtracts 253,540 Piltrates 286 Supernatants 384 Autolysates 388,397,406 Coli Boivin (01-02) extracts 544,5,9, 550,552 phage sensitivity 543 (of Hershey) Lactose mtant run 546ng Biocehmical mutants 548 Uronide tests 548. ZYGOTE AHALYSIS Complementray 372,378, 380, 381,385, 405, 408 Supplementary agnor 9 $09 , 415, 483, 487 Tsolation SALMONELLA Nutrition: %09, 311,323,324, 327532953305 35325354 Sex: $10, 525, 342, 336, 346 Mutants 322, %305(reversion) Lysogensis?, 343 OTHER ORGANISMS 8. pombe mutrition 251 §. cerevisiae haploid 329,333,341 Other Organis:.s, contd. Proteus(Dienes}: nutrition 267 mutants 291,299 B. mycoides 283#isolation as contaminant) Salmonella: stocks read. 303 nutrition-81,53,54,-S11. 303 see SALMONELLA Staph. flavocyanea Nutr. 317 Serratia, Phytomonas,Staph, Salmonella drug resistance 454,485. LYOPHILZ#ZED STOCKS Sept. 9, 1947 E. coli K-12 & 58 derivatives: K-12 wild ie 58 desthiobiotin & 58-161 " .~ methionine — -178 Y.E. -278 Pa alanine -309 cystine ~336 isoleucine -580 thiamin - 593 thiazole -610 thiazole ~741 histidine ~-2651 proline -3214 proline - 3232 - 3356 meth 4447 ? 4899 pk. alanine 5030 tyrosine 5255 proline S 5273 adenine . 5298 indole te Heurie 5417 uracil “ 5450 proline 5580 A.A. 5631 adenine ,. guanine” 5036 indole 5898 methionine 6049 proline 6177 6313 threonine 6314 PR. alanine 6317 proline (glut.) E.coli 679 and derivatives: 679 " 183 440 447 455 662 680 684 threonine 2? ? 2 ? glutamic acid 9 9 E.coli Lampen strain 15 derivatives: L-1 L-2 L-3 arginine IL-4 meth. 145 threonine L-6 proline L-7 lysine 15 L-171 " 18-15 L 171 methionine 65-489 lysine E.coli Bo Xl A-1 A-2 (7-415) B/R -R 7 E-coli Yale mutants ; SALMONELLA Sl paratyphi A meth, tryp $4 cholera suis meth $20 typhi’murium monophasic pm” ( IV? V? i..) phase 1 + $36 gallinarum By 6u S$ 37 dublin Bl $42 parapyphi A tryp $45 enteritidis ornitnine S$- 50 S-5} §-52 paratyphi B req? $55 cholerae suis tryptcysti S- 56 " " tyrosine, biotin S-61 typhimurium IV variant meth s7o. " #&S --SH all Y- ACETOBACTER M.A. ll series: 1 (ATCC 6$32) proline A Wa. 6.2 ("+ 2 " " meth. SA -resistant 11.2 90 werine or gly 3 (L-15) leucine or isoleucine 04 236 glycine 4 tt n 5 318 leuc 5 tt tt 9 threothine,leucine, pab 10 n Bl PHAGE ... For continuation of this list, see Y list) 2-3 RoIvan C1 T-3 Ce 7-4 te B. subtilis r- ee T- 7 hist Staph. flavocysnae Shigella Hu (Hutner) uracil 79-30-2 (Weil) Flexner V 66-1-410 " " TI Phytomonas tumefaciens * Proteus D #3: - 38 -57 -63 39 «458 «(65 40 61 66 44 62 74 pontelle at Yee STANFORD NEUROSPORA BIOCHEMICALS: 4S 1A + 33050 valine A 298 + 33757 leuc A,a 193A pab Bx "-4637 A 299 sitophila: Bg ~15300 A 605a serine 34486 chol. a 830A pab "15300 a 1090 sitoph: thiazole -34508 Aja 1298 A uracil -37401 A,a 1633 A pab 534547 tryp A "15300 A,a 35307 pab A ~4894 a pab-meth 35420 tryp a 3416a nic 358k0 isoleuc,val. 4540A7@ nic 36115 ~—spab -10575 nic-tryp a 36607 tryp 4545 A,a lg@sine 36703 arg. "5531 "nan 37401 inos A,a -15300 A,a -al ~5531 A,a -37401 A,a ~ihositol -"-15300 A 4637 A,a alb-1 (transl) 37803 B6 A711 a isoleuc,& valine 37906 tryp 4894 a meth. 37907 " -15300A,a 38113 pab -34608 A,a aurescent 38704 valine ? ~39401 a inos 38722 tryp 5359 A,a pab 39113 nic 5531 Asa pan 39115 tryp ~4540 A - n ic 39303 nic -15300 a 39501-1452 val,tryp? -37401 A 39701 tryp 5801 A scugbo 39705 isoleuc,val 8839-4637A leucine 39709-4637 " " 9185 A B1 39801 tryp 10575 A tryp 40006 a ~39401 "mic 43302 nic 15300 A,a 44008 tryp 16117 A,a isoleuc,val.’ 44008-65001 " 17084 a pyrimidine & thi4#Q20 " -37803 a - B6 £A2B -44602 a - " 44210 " 453 18558 A thiazole 44602 Bo -34486 -choline -3740lA,a 21848 A pab 44706 tryp 21850 A rr 44707 a 21863 A proline 44801 nic 27947 a. arginine 44802 29997 a " 45210 tryp i 33026 a valine 48502 " 45203 " 45304 " 45503 " © Oo > > PPS Or POO > EM >> > >> % HOVN VO PEO HM Se FH Misc, Neurospora: $6201 val ? ARKKK 25 Abbot 4 At" 12 h 0 Aiton E 5297 977 R_ L N. sit: Sands H.ss F.28 56.7 N. tetra. 4+ 4h Or OM 0 >> w> aaa wa \o 4545~- 5531-15300 51602-4545-37401-15300 fn =-5531-. 1" it 10575=-37401-153500 G 27 A al-pan-inos-B2-tryp G37 a New Yale mutants: Y-14927 lys a -16050 col. A 16059 lys A 16424 aden a 16329 pa alan A & 16349 cyst,meth a,A 16351 aden, hypox. A 163 67 cyst,meth 16479 pab veces inos 16603 , XXX 16631 eee 16744 meth 16470 leuc. 16641 meth 16695 pan 16644 16747 meth 16730 aden, hypox 16796 pab > b> b> b> be > > > > > Penicillium 3169 proline 4769 histidine 6155 arginine 6549 hist 7286 unknown 7288 R 7307 hist 9756 arg 9929 prol 10099 ph alan. 10259 unknown 10283 hist 11117 n 32044 unknown 32179 arg 40102 hi st 41272 arg 47D17 hist 50265 isoleuc 51775 e-m? (i-v?) 52204 pab 52997 lys 60297 choline 69441 hist 81414 " B4248 unknown k¢kk 84886 hist B5880 " Bak 86842 7 89172 " 96730 " 97054 " Absidia glauca (Giles) od LO hist 50 " 1200 167 1571 pan-ye. 1643 "aden 1891 ne 2775 lys 2790 hist 2828 tryp 196 r” STANFORD /NEEROSPORA (con't) 46109 tryp A 46404 7 a 46405 " a 46406 " A 46415 " A 46423 isoleuc,val A 46807 tt cA "T 46808 " a 47101 " a 47203 trypto A 47317 " A 47711 i-v a 47808 tryp? a 48009 7 A 48306 7 A 48501 valine A 48613 tryp A 48614 " A 4861 5 n "1 50005-5231 pyrim&thiaz. a 51602 B2-temp A, a "-37401 A 56501 B1 a &% 65001 nic,tryp a 65001-2198 ? A ~3416 A 65205 txryp A 65612 " 66110 " a 67601 val A 70307 tryp A 71103 isoleuc A 71301 pab a 75001 tryp A,a ~39401 A 75102 a 80801 v A 81001 " a 85902 thiazole ? A YALE BIOCHEMICALS: 602 albino a 1093 lys A 1866 " a 1870 " a 1879 " a 1937 YE a 1943 YE a 2170 al A 2171 al a 2198 tryp : 2298 B6 ? 2299 ye a 2329 BO a leucine A 3284 ve a 2840 2887 3261 3317 3494 3522 3786 3791 4195 4246 4518 4617 4750 4815 4840 4905 4927 5015 6073 6279 6516 6516 6603 7110 IAB 7548 7582 8228 8552 10626 10654 12239 12504 12964 14927 16329 16331 16424 30005 goo0lod 30014 Zoo 17) 3025! YALE BIOCHEMICALS; meth meth chol cyst/meth ng. f meth or + meth he he he Bl meth ye vit Vit he meth tt ye pab cyst ,meth he xxe vit.color lys al? ye yx yx meth yna lys Pa trypt aden PO Pe OO Eww ph POP Mf © & oH © b> fo 9 © P & Mise. Neurosp.ra : | New Yale Mutants; $6201 val? A x Abbot 4 + MA 14927 lys 12 + A 16050 colonial Chilton + a 16059 lys Sy F8 + isogen A,a Gat aden ph.alan E 5297 a 16349 cyst, meth 977 R L a 16351 aden, hypox 16367 cyst, meth N.sit. Sands PC A sea . pab HSSF . 28 =P s 56.7 mo a 16603 meth, cyst N. tetra sg ? seria aden 4545- 5531-15300 16470 leuc 51602-4545-37401-15300 1 eet meth " -5531- bf tt a pan ao? heh ve 732300 t 16747 meth Oot al-pan-inos-B2- TYP 16739 a hypox Penicillium 47000Oist 17017 shi st 6155 are 50265 isoleuc 6549 hist 51775 c-m? (k-v)? ea ne 33997 sys 7307 hist rad choline 9728 rel 69441 hist 9929 pro BATA s 10099 ph. alan. 84248 ee 10259 unknown 84886 hist 10285 hist r 11117 " ere t 32044 unknown 50173 . 32179 hk arg 96730 . 40102 hist te f Absidia glauca (Giles) : * 2775 lys 136 Pet 2790 hist 50 is 2828 tryp 1200 167 LoTL pan-YE 1643 " aden 1891 "2? 3738 x bo» © > bP © ip i ib b> bp bp > > YEAST Eremothecium AShbyii # 2 Sacch, cerev ai # 59 isiae (Lindegren) 93- 1C # £haploid Y-Yo (93-1¢) " x$r 15 19 22 24 30 35 37 39 43 48 49 ao 115 122 2 5 8 26 28 29 31 125 133 Schizosaccha tt orig. biotin, pan, pab romyces Pombe ( Wickerham) octosporus Phycomyces + 4B Summary of Y- stocks 51. 27h, Y39 UV Il “52 256-1 22h-1 Uv it : §3 335—2 Yio uv EMB~Lac = $4, TREFFERS 58-161 selection * 55 YhO X X53 recombination * 57 ¥53 seelection with T3 (sic) * 58-62 360 discarded * 63 discarded a #64 360 153 selection with Ti *65 360 discarded; proabably a contaminant % 66 360 ¥53 selection with TP * 67 360 ¥53 selection with T7 * 68 366 56-161 UV inspection *69 395 YO uv inspection *70 396 53 UV inspection on EMB ®7l 3% ¥53 uv inspection *7g 4 =405-1 Y53 X Y4O recomsination *® 73 405-3 do. do * 7h 405-4) do, ao, #75 405-2 discard *7 ~ 153 spontaneous; slection * 77 430 ¥64 BD. selection * 78 430 58-161 sp selection 79 «436. =Y77 sp sel * 80 A34 Y40 HN2 sel on ENB #81 discard 436:glycerol enrichment 82 443 ¥53 sp sel * 83 443 78 sp sel & 443 like & 85 YL40 X Y53 recombination * 8 456 Y53 selection with Tl * 87 463 LO HN2 sel EMB * 88 1466 ¥53 ap selection #89 YS8@ x Y40 recombination 90 481 Y4O sel 91 xp do. Y40 eel 92 481 Y40 sek @3 461 Y53 sel 94 511 Y53 sel T6 ¥ Sl X¥SY sel T3 97 - ¥53 SP. inspect 98 ¥53 sel 9 ¥53 ael (Tl + T6) 100 «5230s 53 sel (Tlsam col) 10h 521 BBS YVC sel 102 = §2. ¥§3 sel (T1+T4) Histidine, serine Niacin (EMz) Lactose-negative; Threonin, leudine, 5 penicillin-resietant (7) i lactose- prototroph resistant to T1,T3,T5 1 resistant to Tl, T5 resistant to T1,T3,T5 probably fallacious mucoid; sensitive mucoid {no recom: 41;) mucoid less revertible at Lac~ locus leas revertible at Lac- locus Lac~reverted resistant to malachite green 100u/ml resistant to streptomycin, 5 u/ml resist. brilliant green 50u/ml glycercl~negat ive resist streptothricin 5u resist streptothricin 10 u B-Lac-V" resist T1;T5 unstable mucoid lactose-negat ive resist to Chloroacteate 2 mg/ml prototroph; Cla™ remist dodoacetate Ia Cla resistant to aside(As™) Tat Wr V39 (also res. Th,T7,76) less revertibel at Lac- ¥3* (¥¢8) resist to Tl; T5—sens. ao, Ver (already res. T1,T5) [S.c.] rosiet T1Thees cs . CA ON rte be pts SOD TIN Gan MD OSs wag BLS ANY SELL N rer On tS U1 SON roa r On ny f bt. 55- L5e wv ul ~2 Ge OM UlUi ra WO ~ 64. . Tv wy qa N type pera A methi chelera suis put toruan ge ’linarum dublin enteritidia para f para 3 cholerae suis cholerae suis typni suis abortus ovis sendai sendel ” Aneutrophic calmonellas renulrenents mine tryptoohane. Hegel ethionine leucine, cystine leucine cystine leucine, nethionine,cystine (erginine) leucine, SH leucine, cystine leucine, cystine thienine thiamine tryptophane ~ (uses indole, patmuthray.) ornithine fuses arg; citr also) teyptephenes. Brot; feromeek; (stuneqrtne) “TRYPTOPHAME, eystine ladagts) tyrosine, on trotm ! ort ! slow prytoT rep L. ty phi-auriui area #B typhi Mruviun St NetHiowne. Phot we $ HH. uv. mulanf of S20. ebaeSro = tigglat-tmcerienn ; od cab Wp Say S dpe thee pen A Liss a / 2 pain D bz | hdiea ui? le TE, ) eet wre cw as t one Tim xr 49, P49 E plea mse. 1) (Blt It glia vue, wes 7 é _ 7 tavern VI vi Vivi . 43 8 1138 py" | § spsndlen dee 4 mupord vive jt (7? f ne man <+ m, oT 4:%,> e,h., Wi, 3 42,3 ry Hee te ‘Pabedes bet’ Be our Oo. Chx q * 2. jbo poerit. , < »t V2 23 vy at % 33 28 +1 jo af 32 $3 FY 3 g6 37 28 42,3 bl + wil me Honme , Pry plop be ue +* - methionine, HEY fo th” tte +t — we leweine, 1 olane, hasta. (met) ae. 7 leucine farcg,neth,) cast swe. a lewetne, Qh ainine , meHroume Chee) oastt : cu leucine, Hreleeine, meth, | “OK a, bene. leucine; cysTine _ leucine “Cave, eyrtme Ghreara, le) cr . o tee S Lf “seseies [pas Pecatypla 4 y PAS & a )- R Fe ie a Re )4 " " 1S * . lé | ” Ww tale V8 eT OL AneLin S37 nr arretabercs UG 41S lecathecclan, \ Y?/ if MAE nae SYfo hi at gm BST Ye pla cai Ms Oe faa tent YY yi arbor +b ie tole WIE = adebeisle 3YF6 urconcticrclee 3739 fersmne— YY 3573 OH. oF 3576 plan 57 aty 3 lé SY aa nerfed J ‘ 3 ss 22 apne {! 27 x 7 3 LZ Paw ~ 49 45 awnhachha q{ > * wou : Ty 5 pen A qf 33 pe uf 18 . oO pans J} / Sz Zo te 53 4 " 5{ 22 ss 3! hdl 56 30 Zs Vy ped eat 4a, $ SAt MONELLA- MUTAMT FYPES. MAL mnethreurmne ~ eusndk hegp try tare LEuciwe , Rodeo aE beeProme re OY T vx LEuciwe,, CYSTINE LEUCINE [ARSIMINE INE THioviE , CYSTING LEUCINE , _#¥S, Leuciwe, Es Tine LEUCME CYST) VE t+ #3 ++ +t a 24 ye ++ ++ ++ tt ++ te ++ R. ++. ++ — i Thiamine TA (Am 1HEe . 1 At Le a TRYP To PHANE PRS TNTNE:. OAN THIVDE Ty resine ; £ nt. cir Virus Straws T-C. Fon, Derneree . ren 230 6 frofag. Prumd tartunninotd ahd. fron Oeaneee ’ Pretale