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CH,OH CH cHo-P —-Hig ~> _ ; Wp Cc-oP Coot PGA Epp - Ufa Sp eg et ? fee werd 6./ x07 7.>Y. . tt | 7 ic ne) iy Lap i tr vl ches > hind p° Ma My dingle ces abt hiatal from Cis pT GP PR Mf - om A pleces “f 37 aff * ced Wher the (med © Me (pa at fp) hee 2aeAre vadug , able, 4) nar. c "4 > [fo ; a. adel ling whe ae beaf yo 4. 4 > fp -fA vy Le O Dp” Books bei ory 3 A r / C /* ) i / if j Are, Khar Ko plesplal . aa ay hawgdre tt CQ acd rh y wo lf nn v wh ; «hy ie s oy 5 “4 oe é pe ots van ey ie Led ue ee - oe ir oe é ; ch ( i 7 Ms 7 . roy i f : - be ‘ ey ! “y ‘ ; f : lav Pei hee 12. “yy he! ig) uh O yO a Tees Q a he, ‘ag 1 Tow ue j EC / i a a uf fee t \ 4 ] fo / j oy ar as meee (1904) sca Drs ene ey aeenner TO? MK Mek wond he meted See I. IT. Iit The utLiization of lactose by Escherichia coli-mutabile. Deere,C.J., Dikaney, Anna De, and Michelson, I.D. J. Bact. 31: 625-633 (1936). White form of Ecm uses very little lactose (determined as reducing sugar with Cu) before the red forms appear. NH, production indicates that amino acids are used as C source &f lactose is vailable The lactase activity of Escherichia coli-mutabile. ib. 37: 355-363 (1939). Used Shaffer-Somogyi (JBC 100:695-713 '33) method, with Reagent # 50 and 15 minutes heating. Thymol used to sterilize heavy cell suspensions (req. 1 hr.) Dry cells prepared after Morrison & Hisey (JBC 117: 6943-706). Substrate was 50 ml 3% lactose in 1% acacia an M/10 P buffer 7.0-7.2. Dried cells suspended in 25 ml 2% acacia in .2M P buffer, 10-20 mg thymol added and incub. 37 1-1} h. 25 ce. 1% lactose added, and samples taken for analysis. «01% Cu used to stop enzyme action. Activity expressed as u- 205 mg lactose split / 12 h/ mg. Lacy grown on lactose had activity ca 2.8 ig grown on lactose; 0.2 on plain agar, O.1 on glucose. Lac- had activity of 1.0 on lactose, etc. on others. No dif- ference whether dried or not. These values characterize the Lac- itself, as no Lac/ were seen at this interval, on Endo(ts agar. On the activation of the lactase of Escherichia coli-mrtabile. Deere, C.J. Je Bact. 37: 473-483. "Earlier experiments led us to believe that the antiseptics employed "activated" the lactase which was present, but inactive, in living growing cultures of the non-lactose~fermening (white) form." Later found that drying would also activate lactase while only partially inhibiting glycolysis, so that Qoo might increase Garrett white: /plain agar: Wet: Lac 11.7 Dry: 307 Glu 139 91.7 /Lac Wet: Lac 19 72.6 Glu 136 132 -- 9 Red: /plain Lac 1922 42.3 Glu 117 88.9 Red: /Lac Lac 128 1.8 This prep. was obvi- Glu =~ 1.9 ously overdried. — 7 but may have been Ex tracts of dried cells contained demonstrable lactase. too acid. No valid test was made of the possibility of lactase activation in Lac’, but he concluded that adaptation was based upon increased permeability rather than increased enzyme.