Ay - Kell Fd darpenrenes 7% fatiat oSayk “ Tlbeca) > bcadeale foster at | o 3 /) / apeckie Q 7 Gung) wll 20 16 ¢ Ww f Ly é 7 30 (o “ x O 60 oO GO OQ J20 a [fo O. i/9f7 PH. Pepead. poms 20 5 Yio Mufeliny Kern. 77 ye 84d 7. an TS. fade Yro Y 1 36 yx Lop = 7,97 ¢f,, ebfras ploter oe w/e fp © tend - Peibiele © peng 903 7 Q pal- Foye} 2 a) Le , 4+ ALC . PrekeqonW75 3 @ tad-. Fae. - - g 4 G@ + ad ; oy Sy + @® +t+ad- Retualn <0 pct 05 te C @ taf- ——— 7+ oe 36 plakor x = Tor els 1 @ +uds- W10g - 00/04 2. @ Reeder 2. @) + amd + (@). WOK Cdt{ I +4. See 197, 1 | ar S. @ 4 rag Cd 112 = ctf > +e Utilization tests 80 Jan 9, 1948. Lactose analogues 1% EMB b-Me-galact b-N-butyl gala. 0-Cresyl-—b-galac - Y1O —_ Lat +h te aie bua, % 153 bey th a O phedf ¥35 bac, - —_ — - Phraagee nailed YAS Lee, - — — — Heldrastactok, rolls tburrced be ef loping 2 jah raffacet| Futre Huh (Mol YYo lu ~| ree vbross-*est Lactose ~wutants &] January 4, 194%. Inoculate YP broths with foilowing: Y¥53 (Lacy~) and: Cross each on three plates. A&.( fopotrns +) ao W~-30 . mm 7 2 #35 +t 3 . 3 ee A Lileg 635. Ca Sito. “4 4a = A@® -— pifriot utd! a) W-43 ‘hoo + LihebS. Chor < ilo. ; sinehd 7 W-45 t+ Yr - "a. Ses ¢! 8--———— W477 9 W-48 ‘lou + 10 +65 - AL - Dit eelay 10 fs 160. darvest and mix cells. Plate dilute on Es—Lac(B)). oe Vive pwn te be elleta uth $2. lac,- - a) W3s, wus ot lac theontoraty. b) Wye, Wr, VHS WHS JbS.- ta. (%le bact Kecontuute . c) Y 53. (8 7 >) . deyrreal date ai VS) ipcce Eta e lite a bene Streaben tA eth be - aud Mal— wachenils frvneebectel sial tose segregation: Effect of preadaptation on maltose. FD “anuary 8, 1948 .Prepare inocula overnight in YP broth, Y40 10 AM add 2-3 ml to YP-maltosa (4,B) and YP-glucose (C,D) broths. Incubate W-1 similarly in YP for five hours to 2 PM Cultures of Y-40O are actively producing gas at this time. as and cross samples of A,B,C,D, with W-1. Plate on synthetic EM-Maltose(B, ). Sela wrt. Me OMe A 5, Count -“ A. (112) 4 «30 ° Z 768 eo BZ ag o 6 13 / A 156 S 9 24 e O 5 eS y> / 2. CY ! __ yg pt 2. 3.099 "Jo B 6 6& Oo /7 2 /2- OT Zz ne {UX 2FY UW 1 (0 / JO AST za 12> . | Yb 2023 2 74 2.28%, Gj g - wag ef fi 3y8o 357) 2.548 Po /} | Cs (cr) N- M+. S / 3 Aol 2. 2 47 eS 3. a JOG 2 yg OS ‘. : 26 7 b. L sa l ?, 2 72 / g, © 7T/ © 4. Ze /0/9. wal: sodf D: [@2 I, 26F) ) 3 2/3 3 3 fos / / 357 2 a 16s7 1S 2 ae ee u | JP WD 3, 63 213) 2/94, vA 2 PTI VMNL +. Con foacan, 7 . | ! 4 | cae" 3s | | 3806 Auteead , 6S 213) 2194, —— ! om fof | boyd 41 3¥8O' 357 L Fel te »—~ 154 sert) 5 765 —— - § tL ~ Nem: Mrhr ~ SIS 021 % cae S24. Y1LO5 Mutation run: Lac attempts Jan 12, 1948 Irradiate .1 ml per plate (ffacKMB) 9 secs. under Hanovia. 71 plates x ca. 30 colonies or 2000 colonies. 3 suspicious colonies streaked out: l: so Gross-test Y1O— mutants & SS Jan. 13, 1948. Plate mixtures on Lac tose-EuisB; : Y87 (Lac4—) W-45 (Lacy~) eo & . 153 O18) We, et feel as wpheiaLen wa W108 We pe lac, - W-112 a VAS eet we Un Mal tose EMSBy 3 a b W56 (Mal, —) W-60 (ifal 5) W-1 (rie) tre Hely-; Wl w-se. W95 aos. ++ Naly - 1: W-6o W-%6 yt ++" NaQy ~ all thurs. 98 = (i:o00) Hb WOO = - av (in02 “ th M104, = ++ W106 ~ et “Gebel. +t > [:}0e He f:/o Ht = magna taeraal coy ys ~P 178 Sourbaak rr illalim 38 a Wo ' Mo p | wile 2. WO oper 14 Dagalicn wse = Ua. oe vow oe. Jo} sown pin WIS f ; | wth p ; vfow Ep Polysaccharide Utilization Jan 10 ff 1948 Test strains indicated on T(m) plus .05% substrate. As Enulin . We55 Apf (39) P12 (48h) - - —_—~— B. "Bacterial Dextran" = Lot L-10 from fire = K.P.Link — ~— Inoe. P12 C. 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