Aeiecto, fr J Utegae. T ie a by . Oc? 26 1497. Pegaace (oak 2 Suc ae I lopap loa ecb te petate oll Baan Canl Pag Arubccate, Base. 29 vriabita Ch wy strato 2P] $P28 A. YS3. rofre /t/7. WN. SP. aw ance - a. t 3. 4. 5- ¢. B.s6-lt. W tofye /7. 5°. h ) 1 fy ONC, (-) AZ waren . ae IRY Pao PY. fAd) wart. re Seceurse EHS. <> WoS 0 ~ ® Y/0oG . +. A bua y 4. clot At . ik kt di K , “Leal YS3 fem, an 1 Snk /25Ht YP best, ank fw 3 x [Crm VPapar vlanks olue Ht. a lome Nfio phrep lade bof Ft ph 6,0. Uk 20 HW 2 tetarg rusted [ u et), We du fo 10 bff LY shed 30 menuler | nh lle sal ria ath pip bre (Onl 1% hee lik veal alr 2Simk YR [othe and mmubeloe pp Vereen fa puted debtor, “eaadk bn 7 hacker Serra’ ~> Ca. jow Ctr k : p> = Ge to"[0% = a “ ecccorele petd | V har. 0 /0, roo. coat hab TI TH TO OTT bac Wufr. 3 } ~t wh Ss” R § S — -T-+L- Bt. - ifort”: -2 R’ nt § S* ~ -3(W 3” R 4 5 — 2 MD pers, {0 R R ne R + 4 . 106 ‘ R a? ver” + Prete K-12 , + jofr4/4?. pad yp tn ee Se pleteca | HAM. (orig hamnerpliin noted o ey SI?) fee UDX 20 10 goo Obra j re yates ~ woot url aleeverty rat calor wba wed Lisuienalle 1 ght pope van cata } ee tod foe Cth shady 4-H. NAT ely FERN NTATION TESTS: 5 Oct. 28, 1947. Test the cultures indicated on the following EMB: yrs ye 58-16l | ¥54 YFt 5F Ib! AEX “ &lactose tr | 8 hag: ve _ + Rhamnose + > a Inulin Velibiose — ae a-methyl glucoside S - a + Yr Cellobiose a on Cac0z (.1% ) peptone apart | Tulcitol Lactose M mgr clang Urrard feet Clomus 2p dittonh. maby! ‘|Popen atk + ep Fr alle 4 Ptiippelt f é eS pe (Tit bel! Lartoarr. a Br ihe ty! sorbitol sucrose Ethyl Butyrate Ethyl Butyrate (add "aéterile” to hot medium. Ch glnorr emf decal Ceres, Hellrore wW-l wW- 3, + Slrakes pt a fava: bald 4 erty . Morwreuer Hees -t4 4 Finke rk the papithe | Y P 3l. YS% 2b rnin an VET + S@-1614 Dube bf +. [ daslestoh i wil yeleti' dade ty 1 Hulbert slows t olf? a ~. Papell (2) 25 SPI ancl YPT 2? Caton, druce (hv ; atin " é T(m) + hndecatd t by SAG deal ia) Gubtliyelake + TL, Yd... 4° cof Able, 37°. OG ym +ding. lam +4 +G \ elle tq + G | lW-1l — + + | 4 Eo tt tr ee Hote YD, ~ H thee 44 + $e 44+ + +H 44 Succsen 4 W-1 t + YS, bt te +h oO . S8-16) - ~ Ae tr- Ot Net ° b 4 * 1 C Cre Sue 16 o M Mtaw dit 46 CC Ch + qo + + ABlerwun W—-Vom 44 a +40 + 0 +9 K oe Fe te tO So WSR - tet py wee ete wk fie Otte SS-16( = tH + o& wee ‘0 in he MSG o- 44 + t yoy yank can x , 7 ee LE po, hee 98 ~ 16 | at YP berth. 2. P50 Hare toed ramp ay / arch Miro Leyte 4 6, nq, To tah pve fede ip 4 ee RAGES BOR 4 Pony eed FS py b: 2302 - Ss) 70+ ti See at 3:0 | ahi fre. IS mam, ss oe a, cards le 2 Fink uP Seca “Nel nr vy bles, kellie,» 7 S> fo" >t J. pS 68 Specnelh Std : Lo 4 Pticl aurwocs | aha, | a Loe, bel. ond Mel Cplalorr ATT are tolorrese dtd ay 300 + 400 —y cabn: | pect te bt net ucts tp — ta loo / f ant VU, Cepurlill ¢ er are ls mad A ? . 3 the BP ind bo ay? laces 37 he Tit-T, ad nest Vt! » Ca B00 tad ples ae ss 12 LE halla 30,g00. ca 55,000 wrboin neon 0 Tl {- are re 1:4I- —356. f Murrecrim fle + Mek nn Wak wd! = 3 Stisals od pepeble 7 eles wh ED m bac medio bins 41 Melt. ENO cam, Udo the mn regia pate , OS had sorsee Wel, Lae. BUlae-. Pap ble i stuceke ? Me. Whi, MO Ne. edd lu->. Lae All -, L hey Hod Ate -! whois ) - [Leatials A 6, WW ~f. Recrtd fobs fond haus ond sreuanbu lo— 33 w->- (Matt) at — ; peg ble aunt Hoks - we Vee) + aud fut, ~ Wat Maa bead + fececk fA, ined . S 3 -/6 t a /. q ALS [0m saantef Eton /Omk 58-46) onad =2y/o Hho pH 6.0. Tce hoo lanl hoped (ton "Pet a bls at Cong coum = wwe [nl x Jo O furne wana s 3x70" | 60 wm inary = 2 x10°. p> = 3+. [vee ae: barns jo%, cerwnet for tele 10 nuns. aA 1%; wher tale opp, Vows AV, aul BO nurs. buetiurrk /O Uys. A 1947. 4? Pl- be al ad hen ge pete f Gph T ante NE Y cd P) Trual = 2% Hw} a plegh te “4 ‘ne. ». 440 | honey. te Lf VPLS. LS o. ZA bac. (0 pf. £20 ef ~ nt) Ay cite fens bh Oe — 5 alk Lae +. ta $0% Jrowee art bae — ecustct) ont, Mele cots fora. hal p Cole rater with, Hla nT Cet usta patie Pyle 3 colori #bril wWd0,w3l,WO32.. Halt. loxyo =~ 6Y0 ferk%- I Aewrenplicmen f vice Aad, vdherr baboon. Seth Ore +- and hen « ce (Loreeprale te tere ??) | Lal W%xvdI ~~ Tow. Wo reefs . carn Het (1¥ 20 ~ \460 Ws ceceba | [20 73 * 36 > 7 vm Wo ak . y . tro 34 [7 ot 9 Mihai bsp 7 A. Saleteae sadbaits on geheclose EMG. re + “¢GYf Th mister © witha for tptcal ial * 1-2. Bale ey mw t-l. Wek ly by WwW 4 7-3 de. BY to > et Cb i Ne die 7-5 do F-l. PM Ww - : 7-6, Po, py wl. 1-7, Ao 7-2, Br. Ww 8, B, haere nla | 7-1 Typecal bee ~ ast b _ Ww ~4 Fry De Ln. W/o 2-19 Le Pop Ww 7-14 De + Tere bee + colonies AU 7-15 Do. w ~/3 7-16 D . wtf 7-17 De | WS 1-19 De Color Sevableal, eet om husury x bode wool 2 7-20. 1-22 4 MALTOSE | - . fleao xt +. BM V7 -¥), ee ALA posed Sore Mel 7-42, 7-93. 7T- UY, COSY 7~ 76. 7-47 7-78 Cl4s z Tho). Pema by empauren 7 r(4+N) 5 8-(6f. by BA LN). Ky BY De. Ma luo -/4 tu -27] tu "2mW WW - 2% tunby weg, = be tR tr tr t+ rb hs orale, Hrar, HH th tt t (slr) + (atw) base, 0 toy Che rele pa “eh, ad JO. T bees weer, hocctrey ot (Oo | “0 Nor 'Y7. Ke fear ade tebe ay web hy here ite both fa a Ct abe Eth foray eters. thal A gas Maur t arbors | 2? Aachen | Helin Bhig 2? A. p. Flalaclore rchea - User. 4, 1497. Em6- Ral I/- Mee Ih Te lps [ °/o 0.3% Vd-Auie B 1% Yio 1%. Sead ont 98+ G? others. PY. A Mo Lerriny barry » fasge ¢ seal! Mereees UP ar aatrler C. C luge + mretlehonnad p. to diner. 13 we “ Vow FA, (44 T. Mead Rese (60. og SEY, A) mB pas bec tend pling. veecbiele VY heuer ‘eg ‘tapbed fen yi’ | A. SB-161 vai) - SOpl x 72eks. my bac > Le . we). > Tph x00 he wm Bah = ¥500 feat Ra derriell Aneta, . Sd. Mr. (447. Sec 1A fo 7 Telvn S . Ah fheke ro WL bocrnns facet) te: i Ab A) 9 SHbl : |. 12m Tly) & Br | 2. Mound Tl) + gine 0s Co +4 3. db. wllbore H . de Atecey ae = 4 - co. deo Sueceree. Pool. + K t de. Seeewwte Mor + do J rach ghee conche * lo -t. 8 dy. suceere TLE, Popenf. o /6 Morr 5, 14YT- U.-V. Kells fare (yofectb) Masovian lap 6" ruse allen Aprnel link x 1x0 ve Coan of 58- bl m EU tore plate, . hicasbea le. 205 rebeta bd. - Cokories Thee, rclawcly a brats v2 > the balls... = > 10? eawe (lien, 3 eat d seeouds, ws lee. Meg S see. > Bic? Li 10 See. Ha serene Tete Lpeno Mpc lage ¢ faye Fy ee “ G0 /0 Aten - ba Cnr sealeaton, tare Med, D0 sec 28 if In, 20 her ps = nn 16> 2M 7 én 3. bure tural” bac coo nelid a7 10 secriS plete . Heb, +4 tonne alr aubes Morr. 1917. ° ‘ . Fanaple form HA peel (mp /ob- 108°) Ab 1) 1% my EHO, w-l wl... we - habeake > 5. - fa cate 5p -/6l — oT W-22r — ey 1) ,osy, C- @ aD ee au, Tlm)+ BY. $8 1) A meblsta 6. AT ° Alo. . “At men FD: yy. . ~ t has ~ _ > * ) Coa +4 Wi +. s) fo taps +t +4+- The bac - W- 30 | 1 Wer. bo, 1947. W-B0 cepiatntome attris bea color what alee 501 of Oe clei fowl crpen appending ruled - Liat S8-b[ 02 Laclese EMR. | 2 eelorin deserted fron fhe. wala meiwtian wree 0 beet: The itmakm wees dest foamy a orale ge Crtoag (54) fo Nope tmealicing bon ly Ab; colonsse foomeloy 2 lacere - Yr duce -EHE Hale (400. byt 1) hi eypt. 10, the ate Lact ile, — war: pote I. ¥ 10 Ae frac’ of beth Hse es 9 / Capes wee Fowl an Cal be ¥ re 16 jth 70 poles fhe Oso OT A). Picks S tlemse fem pale Kyof.7 by lat To aaprrlcee the ayer lect | | . ee tt ihckbs Tae ccs LE, tobnbte x C) tb eap sling Cys. linen Biarlace ent lbac- wheel, dr the bee W->0 Arend Mb Annes These seam eo urhe. . ? } eo eg ae Seg pahnclc ep EE W-3oa. Fa. A. bere Fact Cele 2PC ile YP ind rv B. Do. + | berifedl d bac - (WW 30) duspptresion’ ) A. 2b lorie baer (Lae- < Noo B: tilly | PT. C. tial ot Ae AL allt all + D. B AG: aft wt*. Ar Steckendt 17. Ale ack + br ack +- (1 Tht xmbwalion : (000 and the pepbalion f Hoa © from there pliler dovr uot Aten te aclicfraclitit haptlires . The Lact may bt wrestle, Le the (4g pollecaf wlicbting 58-161 (18 -A) shale be cre acted. D. Sehehan Cyt ifrofy7, My Nook Avcith cabbie of 18A) uth, OS ih Arles augpinren W- 30 farray s ‘an, av WPhroth Hebe nt ictal , tr. AW (yy AAO-A! Stuck 1PAl a, EHO lee Ault Qtf+ - pl [PA14W-%0 mEMB-Lae. (a oo :tg >: — Al2 VigL Ar a+ ghk+ O2.~- ca 20:1, t:1 Ale Ca Wo: Ash Dacian sph esnned ne nf fey Y-v. Witter, 19 Var. C 14/7. ret Vil 58-161 cables mr EM-Heth end Lac eles sale pig = 4 Porerra Gog Lae (10) le nite 9 phi, Sabot a Melt (10) Lo nubeals or rctecs bl * ff fo lng gp C py went gh pty 3 , str vb \ irr pa hae DiS . lh Lee EL. | 32 " a 3 Ai) ° Ab WSS aT : : As Ly 47 1. Ce a i ee Uo Lordeceg 14 . : 242 r£@e— , | w-2> aa a6 1 een ft ad. G& SOf lag i:laet . Wo aeclnn, | | 3. Ca IOI Lace: bac. Ve astlmin 4. QU Lar -. Atrunios Vio {Yd 2. “Oo Near 6, 1947. - P3. Stabe od YS3 7, EM B-teclose — Tia te fryp tone. alt Paget wre duartopeel, fiche pageclhe te tinal 0 arid BPS. Lied r0 plker pre Leer eve. for | obo war td d brna nyell efor. |. bee Few U-v M alon 24 Ur. 81947, Sperndl jo? cols oe yi {oe (61 from YP 22, plore t+ lachee EMC rleler huiediale at plte Ca. (Osees. = Hannra ay lanep at b Sencat % 4. Neblere DY ph x Ore, = 1440 Col rohf Alleles +. badlerg 598. X tq. 150 geneekl colons = Gove tutif | I> plas stlinnsd 14 Uaealy reonsia Soe of, litre ace O,@D Macod. 3 uct acctredl 3.4 a YT need u sbladusteekong ” | 5. *(&)< “ae 34 I:} aie (4+) wo 3b Uniwet pre(— ) Ws Ico ON Hf - (~) w44 4 poe - W349 Sa. 2:t bact: lec Iugfys + Wl b. 2: + — > T. pu- Ww (sbeglef Mba thon 2) GF. tl te Thelac— pushes’ ferwnucl, yet jot teh, , fesucedl fom Y/0, agen J bd, fae. A 7a. WS2 x YS3. Vorremnte, 21, (977. S frebes whucl, are (og + lec+ and’ yel- lec- 2020. bade bast lec ~, ly Wha pessclls shold be Atorstall, Khevace Cire bree’, lhe tpe bact If- thier. befor iT rea. Mt can be contillbleg Clery We crore "beet Gf % bee = 28 ~ urhch abel nob ey gle for I. Fea bh Aeral aryesflens Augsa dco , Mp tity borer of. f P21 Pro thins nt 2Tablro YP tretl, . heechale orm, Ae 37°: ADD. Vtamapir Foal ta. hes Sut yo ne FAM _ . Jao, ek, ey, ln, / /o) au T(6,) elaten . A. Wsax Yer $5 Bo WS3 x yews! A2Y. Seepenel lol yO and pttahind ny lac EMG (i abhese; arvog he thomas o Lact and Lae - popula tgegelnn Uae, Co. 276 Vorvernher 2H ITY 7, 4 Wwsax Ys 3 Loe t+ ot % lee - ¢~-) Stirabe rt woe oe | EN B. WS3nRYSS. lac tG- xcae-~G-). ae ie wd po bb+ fb~ Fea Haw ws ght Alo) g% - A( 6). 20 bar - . ll append toncert 4 FM pater whe. 7 laet. ¢ Shovels of- + aud >— oma fobs fue. Bib). lyiae- te Pieces WSs + ‘oe YS 3 + -~ J g 0) | + Mae Sy ; _ 2 > bac 4 “hr: ; . x Ue feces Mt dl Lagat allept a Ot yelecbrcke bed tah bec brs apis tpt ht 153 - 1 oot bec 1 trie: A (oregalrsmte Thy ddtipes | PUES Pee epee “he pace tal and uvecteorr of IAM, T-b ~ B,- bac tv? wz B,- Marr. Cura B-/4- 7+L+By4 bee ~V% x AULd Ales P,- Lae - types Mechs Crd wer a0 brlace 4, Yyso & B+Y+T- L- B,- lace V, gpoutaet Beet B-N-T-L-BtlactV’ at ah Lael nine chance darter, . Me re, Ieoty ! | | PI | - 1Y ~ AU, (PIGS, IT. ae bec~ (c-¢. sfro). T lcow orton lakes, Cals Hane oct TL RA TLB, Ayrin, pep ae 7d T 2 /d-1 + ~ + a rT 3 vc t ~ aa 7 4+ “y pyned 7 + + x 7 naa " " 6 1573 ft * 6 f wLacolruce § is - + + t 8 T g ITI + ~ + 4 a jo? 1? 72 + ~ t 0 nou7r>ad4 ~ + fl Sl, / totone , Ih 'p. ! _ + + pr TT : 3 Frm 2 ~ + + Yr { Carlos, ye @°-3 ~ + + io / ey Prete. 1? ig.~4 - + + 7 ie af 710 taloreer?- Miche 1-S- 16 )8-S. +. + + é 7 Sruhsaue. Heke | ~ 3, f-H- Au. + PI0 chore. Brely 1-5. Cote pect sae { i a . oa 7 jist, pecceh te mallore rtiod up Umit rg, | SLeeliod pid feat trig Lact eoleus Nev tide Kian ek pg cope eZ 2 Aaa Compeuren Years gee d/ncgace fox ee Kab Myereber a0, 1647. | Mele Mal- lace lee EMB +1%: baler ©. fred - alles. bacbie Y.5.9. tte = ahh- 2. heey, tee alagn | epntan of is Behl (farayn ) ted Mblbar, ¢.f. +44 — elf. 4 (EtA) Meee pefd tH ~ athe Mableie fluuief 4+ + allat (EA) shag x et pus eve te sukble seems. ~ hilo otanelcachrulornes - athe ply he “pony te tee pe bh Pv ee te Ann whe, foe << pf eh ple pudink dtd wll a anole up tacbee “use wleogh nate hit and Mbbee ‘andf ( Fitna, EA. fas, (ef vege {er Mallere lLalore 100%. .0o! 2. Vrr §. 0997. wb ge ed cll, Lit, bl Mofo ee enemtitions. UM Int clbt Im yo fog ous H 0 wok pepe dfs p11 1.0. CLE O38 uk OCP NS “6 pes feet Made «up WIS AM. palate. so thyy N30 ANF. laa! WES ~ — 7 tt — +tt pipe ++ ++ — +44 Lac / tits tO ae np Aaiaez - - - a Unreare ov S/ pe at alle E gen 576, Lhaudlp eto f a with To Ty, ha mys Ie i : peotthiy rep ° of _ go, Ve L 2 Chccoer thasitl “ Ure Heese (Aad tht ta + : lon “4 ee . Nkns nrlia turk aud vthiucon , + 3. i hem ntifandiaccd ie “5 re EMD An oe a tte Ansf au Mitte ay ghee, + frvenk. al ban vei bee oe - lac eotozecs, mn. Ad; ff ge bx 7 cote Ant fea abl 73 . EMP tAcgaal Io: hase, Male -mA sé “Gy. tics te Pr ee — TO tate + ecco erro Ladner tugace, Mf By 713, “| 2Yh. 36 t,. J Uvh. Gk. 58-161. Yf7. 1%, abhor. nw. precieregeci fovipead i faplla . os Sf, arnall difade ~ br Aun - meccoscaget fra pod iden) 01 °%/o Poriporns, lien. 0 rab; crlerscce | ects NI co 4 Attfay hunk / carbon wepglonw tlm vd YET pharor ctinnall, fom fips an al Qe A, YE? fone Ata conus cuties, prope t wluih, witin Aureus. 61%, ni aker geckel& . (DG eager; Mel veg) Koy 33 Gora WsSaxw-l oa, Or, “er b-H-T+lab+ bac +fogdyr Mek + x bers ee bac ~ Maf- . a en, Z " wedi he Me Sete bee 3 f Now. 26, 1477. Mako SP - SEC ra ESL a” » 3p 2 hen Meares 3, 124 2 0h Ei A ke olng deat ten Aiea, My, 44 rae ta Abpjce: baad. if 1 ee pom OT :@ Db. Heals rut Cot f" a ute AAt At beta 4: &. ut ™ acGse c ifs | Ww-2P + W244, . Now. 27, CYT. ed ar ecb 10% pu | cele. . Y 32. ro). ioe . Yi3e: Aeysmme | V, cohen | |, i colores. Vol hulu iy No tolories . Yrd2 . Tle). ?8e + wolf turf. “ Fagen tiacf-val. 2? (00 rely. teeth | ful . Pay Lk. CPO Colonics for Yi3P4 YI42 |. , : To) 7: ( (- E 7 of len UP leer. ned. Os bo = vak - ctv. 463l of ! MT oO: = 2 “4 +t £36 aha ptes . 120 ot % yak tu OK! Tau ening why 98 194 ae “ae wets ths! 4 dt oo: ~ Bh cast t+ loge “er : 132 Oo, ~ faq. a aig. ~ he no Aye. - As Both, A+AG Hy. ad m- Re 133 OA ag at hs a atl, Ftp 2 dee ghe 7. [34 0: a the ar tt 8. 137 O: a sup athy q. 138 Oo: — ay ~ fee ~ AE PF ok. atl on. fo. [39 o: — a4 ~ host ~ athe te OX. Mies Adal . 6 44t. EC +H. J}. VB. 0, -~ (tv ~ Ht Lin tebepe tut afl sae / 1 wy PAF Miya eegpinene ! db apte wR as a f ty 7 Ferahd eek chive Ay Md 96, Ob AVE. Bre. af 37° Yi42 a ey ad aa Vind 44001 ace fac habe, copeobly 158, do. Yiro. gard Y ire, U4 bu efor’ frtecdar 234 free 2, 1997 054, un, Tl) Bm) wd 1% cn EMP. A Aaglee (Chink ~ fron K-P-L.) 2 fru epee ( de.) c WwW 0. 4 Sa, lal fm Barely. Pit. — Conliah Foor whihegation Y arg opel, att to ott" pene es py au Pld egahinn Y x . PIG Card Mecrmrr cy feabrdl, dete = ue. AH .01% P24 Utilization apparently complete. Rate measurements were exceedingly crude. Waxy starch was not utilized to nearly the extent that amylopectih was. This should be repeated for confirmation. Save flasks of amylopectin culture. Exp. terminated this date. a . ee nee x Ba + - ) N16 AM Qh tale Ap. 0x% c+ + aud uad- veel - os, i‘) — t ‘hak. 6 £ ( h ) ° d hee ste Noertee ~ J @ + = fee hae “ —_ te» Lubliicd. J Q@. Utah soiclld lay, eel feop nw vats atcha hin EMS! ~~ — (= 6, albbd) YO bey Mar, Pb yak 4) vy Rey 1997. w-| xto-S3. THL-2,- bec -HA-/ogt x BH- bect Halt (OG- M M- ) Tha) ples bee A IS b- 4H Melt Hel - b) TC 9.) pbc . in © , 7 THA : bet 3 25 Lan of q/. | 13 Me - Lt 2.4 20.3 Lact =~ 22.7 - 3,2 Tf,0 beac —- 2? 77,2 = 6 Mabe y,clerely Clef O-n. dondunith a tb 4, priv eA ol . feet ge P aud Lac. ees ladle any rofeen 4 lie. an Melt. Cheba mack, propetef Va, Pec. 3/977. fF tron Rati Liar fee 42 Ys ati, t, tal 4A Hay vere hlove “yee * torer} Pi bnd— Mt fp bent ler, . ( TP, pate ). Mt iM - / 28S B / a5, ! do } 7? 3 36 4 4 3 3 al 4315-272 / 28°. Mhr= thes = 46%. Teot all Hatt + m taclbaa L Lae - M+ o =< oe Nal + - _ 3 ef le s /3 gq j 220 fhe 272. >” 1b 7 388 22 / 400. e/, 9H) Lac - TH4.I1% 2,475 bee + 10.4% 3.3%, Y/b fact Lae - (og t 20 |. B36 +! bd - ° 4+? © 2o 27 Thee praciude unet conetrnill hy hiahans fot Ve beet wed, crefeln blows vot a abt Mi WA ner W-S3 wes whl at 1V6. furl gu aloo both /o6 + ard tenet ly deo A To prmemnariye | all aval baet ce (hehe The bee~ of YS3+der., Y87 4 hen, Wo are Ya : oN . 7 , ~ UY 3 yy WwW 4h, WY4 ce /Obgehl + ; ( be Lae 035556 ) : / Cll Luspinewnn elaed Peleg? hth Oak oom Pope Tnne war pat or 1 (0) atid >) arucblar Eri InEMh, Mone of was (1alleae +. n> cape mk, Bporrlte Het - On Tho). Vere f IF ' shuld G Mallee , urd beet. 7) tis ate | BA EMO), bac rgeyaling was: (lak. 24th, eto hd. | "0 es Sent colmce une niticd be pocboal |e. a saan fh Bacwele, Hacte, “4 2. | >b00 Wnasltelt. Conepine HL St Leet by bec Ho oO. f x ¥ i yf LJ ) fo tr ). 4, bee thy mye Ute Lif ‘Sh de ( r laAore S- MN E Po) voll be. ov Feces cenacng ploter [Spuigtinen ) “ - 6 . : 5) 7 “ lban oe allah Pond a oa Y > I heansracury, c : ZL : i : , . . d i K {4 aed Loe. hully feasts {litae dg “ A, Yto x wW-l Lact Naklt Lae ~ Nebr ~B—w=o ~ Yb beep Myf x bag At hoe aof bo EN6 -, : pels e, Bin EMC emby Ll Mal Che Neb 7 lene eam ke prcbecdf auto crib be ry eacfrl. hee Tlo) | df? hs Ce. - Vine _t Y f. d Late bce i a4. AA £ pa a ay [* vadky fas in. Ps. “ ue. 10° hehe vile P(.£) phbes (hebeLhin). 1h V.Ly., 1% UE hoe , 1%, Ape on peter, sale’ deregels Corte : sf 7 SI SY iy att FOR ectiye 3) Awe wn weovereal. ‘ + Mt 1 Moree (a by acthuen do Siete pS ms feat fen 6. 1498: Vel + 3 de . }. ~ — 2. WA — 3. EA — 4 +E A 7 5. Ne (G61) + b Vlleee a+ 7 Y.eg. re Ace SFB bole ng. on inks. shh bron YAS Lac ~ aud? YF3 bac ~ 7% heeeenber 4/997. WIS *¥S%. Oy Tlp,) andl Els tae (t,) Gea Ib lace sta S/ an EN. Teiacga eG fault Audfeatinn ferw bee ~ \ Max ke baer eis be Loc, = bee, . ot on the S : Yeh p. on be 4 7 | wo err EN 16. ee ee ge f« ~ ve Fg b «,* o SF -- 4 25 | 14 7 a a 4. 4 >| B e tee* 2% §F vee 7 *% /7 wWdS x YS35. Reged 46 Re pee Lect posse of Ceres | [feats fo for), nena, Grund fom T(A,) ry Lee. EMbase te Pend | phe, oe ay I rs Gut samme avenge sates’ hegevrer, 34 $6 a = sh 67% lace , atices Hee tarelor, (q wa | i] t Helelims unt 90%, Lae -. am Y tHISG 4, txt I? & » tt ee. 264 Be ty 3, 17a, ry . ° .e @ ® e HY] leco : . °. eo? “e e. e * e 47 hago * ® e fy, e Ree, ‘e* 7 pr, a wy eo a? -eoe: ° e ‘¢ teres . . “é e ay we @, . e . ee all .. se: af ~~" "ae oe Parry e. . eo W-2) x ¥ b4 hea EMA, Mbhons Sy sap hen : IP . | B-M~ Th Br Lect Neb-Y x BIMET-L PB, tac - fel," eh Nels | A pura rene whe << 1: ltoNel - Maft ! aD vii Aatl. . 7 e* @&. * ¢ e ov re BL ae . 6 2 e ® woh. - : ° / eo 3°" eo HM Te. Oe 8 a of” oe 8° \ ot es wen eg ro 2 ee, . bal “+ @ Nether, Fatlnart , ekcin Shgeling, v4 Lee 8 (997 a). Keffpnces 3%, Nhhww (he + Seletin (% EHO. Shakrl SP-U/ Aa. Mer RK -~ Gt te tah pl gar pl Bo b). Gute Mgae? / ,Os af Tn) + Ply. VE leer anoles 7 4. a ad {hte SaliwmENh and vine aecoad hale of Ty) tolocc, t+ anud- Cloris. deen. gelekin Sele bees Hafere. fru rueeeenfuf, bal + oo WSSU Teat 153, W-7S vw, wn LA «bette ty | K Plrencake 1% 7 2°/e t+ s% $e 1s. ++ Dhuron os% Lt 1s 2° 5s por" Mepplrmintiny Meemrbmiane Sy bee 4 1447. See (ssa? VYYo x W-l. 7 Plate v. thle m ENB LH (6,). erde, EYSHLk £6, boots fox arcloumns - Conctnel, M(o). Vara Ba + i. 4a 32 o l 48 7 d 22 f v chiar ster Q) 1% nt © 22 «4 2 | 266 2. 4(6,). 13 5S tpl Lip Dez + See . t 7 3 2 ig «6 © 2? ppete frag bell lacr y % 6° wf lt ‘ a 2) oO % Bee APL 1 Lee MY, ¥ Vv, Aur Yee G1 JEL? Ye/o x Wo). ee oe veuplEn iA Sera by mtn Krontrnats — , [tad yi, fo). Malt Ml—- Sell Sun 2 Ye 2 268. 2. H4(6,) $5 VAS § 30S. seu. 31 £29 7 S72 {lan 067 92,50 072 J 20, 0 | About 8%, feces ~t Hable +. 3. bael). laet+ Laee- See. a 94 189 5S 293 ~ 4 328 (646 O17 ef) é Fim LC hee. Lact R LactS lak bar - Ere oe. Nal+. Y 12 IC os v Nok - 4S / 66 7 WP « LPO Ge yore. nlf, “SAD. oe Fann 2, Ce bee. 3 Malt Jo 6 16 cf Mal Yo tal | Melt Ro alt S Habl-R Mab -S. F tom 3.% lal. So pact. I 0 be 3 7O C Lee - O 2 £9 2) BQ, pate. SIA. Mattie & aan y a Play mer a Nellore +. 12 Lar - vy" Mallen + . Bs ogee Jo lLac- UV": 6 haere V,%. Mellerr +. 2r Lex - —_ fe ben th Mhas - lacksre f ' “4a * . Halt RW, | Mab -R . T / q oO te 6 4 oO & QO q 7 6S Te 6b. BS 70 ee” lee oO O02 BF af } 6 oO 6 3 / yf ] } 7 ° oO 2 o 3 IS 3 iy 9 3 é o SI. - YS, ( Q gy we * g lod arent 9a ~ S 2 a - ¥ = 1) DHYmM YA Oy ~~ CulMir Ae m nalbre - _ + 3 . = ’ 8 i, . t:- 272 1S nt : wyy yy 2yY 835: Zo. eel sa | Hes leet: - om, Stak +. 4: [Dlact+: Place — ; 72 +: (0 ~ 3S Lact 19 tee] SY fle aaa bapke conapaccd Co 4S, eagles ragels @ reap dedancce cx Cnet thel frond on the draees of Che bec, V ole. A lyse uo, He Mah Rigany rang fy lactase (6) tommimacle favor abldimeal teresnus & tiple Wt [1 Nee 4, 1497. EMG Melle plate bald fr taseed W- and TI a2 Aportincs cepb thw fob areth weft wcll hifoal Viunerer, Sriaheudt oncsuel, (1) colon Ca ih we Mins. CF At TS. (et 70 SZ [ren nece Cole oflcloes foerenlains 0-49 td) ~| 5 3_ dec. 7, rte 7 Wed 5 (Lac, - Pt Lace x bac, + By -bae- Malf~ ) wet. | plete debetien, 8 EMS lac nate sj} Fo. 68 | 192 ‘nalef fo, Cz, ‘ slich pctec miner thf 8 | pnrierrdle hae - gun fen _ og n. | proenela Le fev ctaresrebrhele | me oF, s “ tL 42 bac betel, BY) ML | Mal + Feat of of Hare Lae evcdibodely on baclpas 15 Mnelurm . in ame wr hear crerreslonns (eathy ¢ a, 4 hed 09 corereead Le Ugerd Har Lac, Lac, ~ . Shelagh jr EMP bar rset regs lll oly lk oS [tte park 53-6 —uste that Cire retonscs une um fap Leena’ hhist ! SY [7 ( Le Ay - Run, “ . ubelen, i - {2os0. Like, [pee = tHorcesr fj 8.« 6.) 15D jAracat te , , e Coote 54, 5. Lac. tee 1D reecld o@ Woe 13, 1947. Decke+ Lehl Meer clones este hth Gre lan 13. a - Wsb Gave t+ W-ST. Ma coe nigtt Reskiinly we T4~(5 Rewre. 64-16 Rew. 17 No aveadsel poration . 1P-20 fare’. 1€: wo (G: wel Wo wé2r- 22. Ar tl 23. @eo lf bo. 15, 997. sj) hewtecake - Aged SYAL. We Leff seat arotul | SY-/, 3 Gee util 7 wre C Apel Conger $ zt w 63 5Y~)f a) Mk He ») bbe, poctiy tical 15. Teruel Bly. Rectal Gee Yl bo Lhes. ahal at. 1h. Reatircele . 2) Keotiah 22, Re hiialh . 23 Tok . ty Woy Us Wst (Atol+ ). Vo Sakrs. Nich lb vlauf. 2 BE = - — We7 tel gg heathy wee + 67? oF wr? 2. i puretabl pack, Y, iat + and~ ~ wW7- t+ WPS 3. ttt tnd ~ ~ WY rs SY, pee 16, 4 74T, a+ W74 b.- W78 Ld - at Wal b.-nx wW8o + wees ? Depoet Prantl ban ¢ S-~ an + tatorrees . 5 uw ey b * Agente, — Carlos ‘ aa Lf there WPS / . ¢ a WIP 2 7 we P &® lac — tMhKhen ty) hae seis,’ Dee. F447. arhcahi, . Breede Tirana bac bianacs m specthaten’ ba C sch fe sdaed fin howe, Eopoed “capone fom pcos Rurruno Y¥53. + + + Lo345 _ sp + + & + + + at + w30 + + + + 4 4 + + + W % - a st a wo + t ot. 187. 4+ + bacwtt © Host +4. Sume Lae ~ a. bee +t €. Lar - b lac ++ 4. bac +4 h. bee — BP lac A. bec - Ts - & x 16 6 ~ | bb Lae c + + f S816] int + ++ w-35 = - ‘ a 4 2% Kup nn ahaa . rr ne 5 wey Se « We dh Bibra te / ° q: . | he: Do, ff b, 1457, Y(320 to Aegmene F/0) +: nor. PIS. ny Ae kor Ff po! 3, MEH ~ 4, EAA ~ y. MtHE AA ~ & He ~ 7 Yay ttt & V,ts - 9. wcHVTS = iO.» tat > ~ 4 Neat Cilacl Mind ?? ng acne, “4 WF-12,3 im: Vale TO) + ' > 3 ) = + . @. FAD t+ oe ~ 3. WA : ~T 4, Vv, Fe Sharhy nan C. i: ae W- j Ist Aig" nd Veron TIO) , 4 aff “(tes we.) erg sce wb A-fA. av ilo) boc, at 22-29" " | i hee, 2°" ( iafoce G wwler meth A I, Yay, - ae 2. Urts. - re. W-l am To) + ae te Le oy glia CAB (R- ednopel fur sharLohnr} TT =—-_ 3. do.+ mater roneree 16 fwd. ann 4. do. t pol lr fwnQ + 4 — YIB2> YO" ntenky . ; u~| So. YWA. Peparating easels BS, Sedum Wercler Lee vy 125k My 0+ Ha 28° Mit weBe aay 50D arte haa Shope, Arnel ectortace of 1S (by. Yalleee ecco mr retatern Ss) bee. S, IGY7, Tet the follrwons 7 brcltatse EB. bb. Lane. Nba + - 1G 6. the Nblsin — : Ww RHE Ww tHt wr+l + W6O wet wssé wtadan +f +H wér tHE LER Ly 6 pee bey wts Dee (P1947, wa" + w- 71 + w-78 + - w-8o 4+. + Uulilon, of YI9Z. SP dee. 17, 1947. To) +. Alt.) ALS, . I. Y. Gy. oe +h “ W2 Care 2s arte (as alrw 2 oe . +t “ than ay ted . 3. 00s %, . # - . yf. 0005 %o ~ “ YMA bar prarrtadunl ~ ok, 5. YWwA. .6% - rae ca. .f —,ol al ; 6. Wilerxe .5% & t 7 _ _ nal Geen Ha, Mupitlirn tghidigreler, (5. alin , ectalhioniz;: Tho). f. ~ 2. ¥.ey.I% 3. Uhre JS Y Wr tare S% 104, 1. &yT. 1, e@ 20k 2H, fl, / x. PLE. pd (fla, (ba. fll. Ceregpoale ] r4Ueacdt nnd jabae un B 2k Ht, 0. De. Kerestue Fela 59 Lac-l and Lac-2 mixtures Yec 16, 1947. dake up 10 mb tubes of lactose 1% BCP broth. Add .5 ml inocula of : Set up 2Pih 6 P16 10 a lp ALS x W-45 - tt W-45 ~ t+ W-54 - a We54 - The Same - WAS & W54 - t+ WAS & W54 - +t. K-12 Hy, K-12 On aun FwWDNE *herefore mixtures of Lac-l and Lac-2 are unable to utilize lactose, although recombimnts are able. ¥ steaalima my bacle rcbably procs Heath + colores Beals Botte yt w-45® lh bun, farts. tecombination of Lac-l and Lac-2 on complete EB medium 61 Yec. 17, 1947. Harvestxte W-45 (Malf Lac-1/ Lac-2—)aak and W~54(fal-Lac-l-Lac2/ vy," ) from fresh YP cultures, and mix at a conc. ca 1010 fml each in water. Store over night in refrigerator. Dilute to 103 / ml. and spread .1 ml on EMB-Lac (NZCase) plates to detect? possible Lacl/Lac2/ recombinants. 12/18 PM. 121 plates x 357/2 totalling ca. 40,000 colonies examined. None were Lac#, This is a control on the reversion of both Lac-l and Lac-2 The recombination rate under these sonditions is apparently too low, 62- Nutrition of Y132 Dez. 18, 1947. PIS Inoc. into T(A) 4 AIG. P19 har i. Ye Extr. 05% $44 - 2. Y. Extr. .05% ++ “ 3. YX Residue We vt — 4. YX Residue .Q5 rT LC _ 5. YX Extract g5% AK ihiwt 4 thea 6. YX Extract 42% - “ de Gelatin jvdiclyeste 5% + + 8. Tomat - a omato = ce p> +4 10. NZTone .3% Hy Lv 12. NZAmine B 05% tt t 13. YNA intact (NaNucl.) $4 * 14. YNA hydr. o§% | - -_ 15. YNA hydr. o§% # YX 05% test tit te for inhibition. Idfeodiiorinocterx 16. —_— - ” 17, M2taae Si + H+ C Mbnn or acti &) 18. CBee Cbe ard 001% oO. Fou a tality no nol deel Ly lbba sider feed spot) (Mgt U2Pase 2] 10g. Yeast Extract Yifco extracted with 40 ml. CHC13 in flask. Separate, evapotate extract and take up in water. Expressed in terms of original yeast content. (Very little material was extracted, perhaps 1~5mg. at most.) W/5-3 LY Plu) + Pry YX ,2Yo 4+ tt NeeV + rt Nigar - th. 63 2-Chloro~4—aminobenzoic acid Inhibtion and resistance mutations Dec. 15, 1947. Prepare plates of T(0) agar with 25 mg% CAB. Do. (0) agar. Spread ca 10“ cells of K-12 on both, incubate 72 hrs. A) T(o) agar: 400 colonies noted b) CAB: ca. 42 colonies noted. However, direct microscopic observation and smear impressions show a large number of "micro-colonies", probably equivalent to the difference between CAB and T90) plates. Each colony contains, as a guess 104 ~ 10? cells. This suggests that Strandskov's observations can be accounte d:for on the basis of spontaneous mutation and selection among the relatively large numbers of cells in the micro~colonies.) Maltose-segregation and supplementary genotypes 64 Dec. 16, 1947. Harvest from YP and cross W-55 x W-54, heterozygous for Lacy, Mal, Sal ,B) as well as V,". Cross on Eils-maltose with .002% glucose added. 4f,. A20. a) Estimate frequency of maltose‘, and of sectored colonies. Score only those where the sectors could be scored accurately. Proportion of Mal / (including sectors). Count sectors as 1 4 andl -. + Sec. - , A 2 57 Melt 2 THT 7 YE 2 f o IS] hak 51245417 5 3Y Pgs 8 Ss. 7 LDR. 12% | 17 Ss si2 Proporthon of sectored to plus colonies: (Score under conditions stated above) Phale See. ha # Jee tf See +. tb 2 j 5 ¥ O ’ 2. 3 © 6 ! uf | O 3 6 > f } / O tf 2 2?— | I { Yr O Ve a 3 0 I } ] / Y {1 d 3 ! a Loy 3 2 oy | 3 f 3 QO wv yf 2 2 2 t Uf 2 3 | 2 2 3 O tL QW 32. [20 (72. | ; 0 30% fhe Mehr colar y i y 2 3 4 2 vigo Harta Nal- Lesyesacd . } * //) : = { vv _ { 64A a20 Score Mal~ segregants re Lac and V1. Also Score Mal f Mal— Lect VU tae V> Car-0" fee -Y> (Vel scored well rn, i e) o,f, 1b 5,4 4 2 , , Ge lanes rertaomnal fo 4 cert mn wal £ bare v4 Lact lac -U" lae ~? “ty Recgue lt Heer ¢ fate, £4 S* ta aa fasta Pick 57 apparently sectored colonies to water N20. Store in refrigerdidor for laver separation. Hecale out 7 "tol 640 scores on ial p/m components of malgtose sectored colonies. Lae p/m and V, r/s Colony DMWNIOUWE WH PF kt .o idal p ms - ps: ms - ms. ps- ms. ps- fas: ms. mr. ps: ps’ mr. ms. ms- ps: ps. ms - Mal g Scoring very clear except where total lysis may have obscured fermentation reading in 22 ( Ysaxys5.) » Mab ~ lee, - v,* x Holt Los +V,— Lac, V, scores of intact colonies: A) Mal / ~ R -S +R + §. Ss uy oO 7 2z 7 O 9 3 uy oO /O 2 of o ' {2 /9 O BT / $8 24 “Rave Fa yf B)Mt- 5 7 | 10 7 $ ° 10 5 6 z S y 7 oO /o f 3 Q eM 15 28 3 H6 [1 = PO 8 A 37. 47 3 $Y _ 160 . . £23.21 Coal ¢€1.97) CV¥E.7I Cegieat wal Fille 5 Ahmed paps. Lr . gs = 26 ( ° Dart. ( Sf Aicclecs pops , i Her iceabue beden’ feu? Mek suk te bliin Marl B! (ne wbicecling with ba), Mibat. D7 v7 » 7> 160. Geld Yhy wey yu Go. Noles Mane F-R anid difresney of +5. A ng. M. bere bee / y, f- _T4 . i. a arth, b. ps 4 O ! “rg N+ [rt - a y, + Te = YZ . 30s Io>L+9$ +» 22 4 fejech a preter terhminand ub yak tay TH. The difference between large and small colony noted on the above survival plates breeds true on the first restreaking on EMB-Lac. Transfer to slants as 67~1 and 67-2 for the large and small respectivelgy. TREHALOSE MUTATION RUNS 68 Dec. 25, 1947. Use same cells of 58-161 as in Exp. 67. Expose .1 ml culture per plate to 15 secs. UV from GE AH-4 lamp, as in 67. EMO - Tretialore pater A). 9 plates. sdost plates, due to faulty pouring, had pitted surfaces and were very unsuitable for scoring. 1 plates, prepared earlier was more satisfactory. this had many (5-10%) colonies which had radial striae suggesting namerous variations affecting intensity of fermentation. Hold plate in refrigerator for later testing. i[3/77. Mo rate food) Maltose Mutation Run Dec. 25, 1947. “et up as in 68. EMB Mal plates. Ca. 11 x 200 or 2200 colonies. 1 sector noted. Pick to slant as 69-1 for later verification. = large and small colony types seen as above. Janr|3 Suehodl as EMS beatend ALL Hel. Mo uuctulas 69 Lactose siutation run Dec. 26, 1947. 2et up as 68. Lac-EMB plates. Killing variable. Ca. 200 scoreable survivors per plate averfage 65 plates, or ca. 13,600 colonies examined. 5 possible sectors. / II 4 core df 2 Wot sehr 3. (e No unrfauta _ ACL lace 4. OD taud-. 5 bee : ® Vo bec rtectrrete Maw Mecctore) . hiclile ato 219778. Shiba, ef Eris 70 wutations re beta-glycosides. 71 Dee 27 1947. Grow W-55 (58-161 salicin pos. mutat,) in YP broth, harvest and cone. to ca. 1010 ml. Irradiate 3 ml. in quartz flask ca 5 secs. rotating at font of Hanovia UV lamp, and inoc. ,5 ml samples into 50 ml T(m) with .05% sugar. 1. Unirr. a. salicing.14) 3f B. Cellobiose - C. b-e Glucoside 34/ <. 5. Cellobiose - C. beiie~Glucoside 37 Incubate 37° Examine Jan 3 48. ~treak out the b-wdeGl cultures on similar kam EvB plates. +his may be slow rather than mutative utilization ,a s has been observed before, 1 ea ie foe pe Piceee OE eth fon proces Mopar an AB8. & ) t ar tamrmtal yng a thas _ rs a Bear. ote 76 (oo) Pap facoe b colniepolelor, £0 genet. Als. 1B (Cbbtewin) wtp bene vechad 114, vlele bes LL) Al3 Fre anges tah acento f Keli’ | Kicbe Cross-lest sini tose mutants Jan 4 1948 6K. i-1l Xx Pesudele A8 (Ppoton # -“ — 1 W-56 - 2 w-58 + Ca tito Malt 3 ii-60 + ca 1:20 mee ey Trae” Pau ra 5 a-71 + Calico 6 W-78 ro ta 150 Mal t. oP ae Wee BO +t 8 we 20 + ca 1.50 wbx YSR ghht Parent +. weSornysz allt abw-l 4 W-S6. b) WS8 Wha W-20, W-7/, W-76. ait deed od Up 0 -_ + wl. OX, w ye. ok, wWw58 ox UW bo. On. w 7h OR. Nuyntigus Shieh nD 78: Mal ce Herel oe 73 trehalose sutation “un Th Jan. 5, 1948 Irradiate Y-53 on Tre-FiB plates, 10 sec. under Hanovia UV lam. ' 10 plates. 10x ca lS> ~ Foo eolorrced . Aeclowee's slipmed fork, heatincd Aeelovny t 2. (2 On > bit og if andr 7 ° fa ise, BP nd Alen b. ~ ‘\ untal options . ( fite’s Way le dua to ‘pda _ - ~ ~ me tw -70 yo- Fr Fee 79 tothe rhe sabe of age See. TS wet, OE teen “ dla ~ tok, pe ee pl Aik, ” . tratadrle ths vp cok. [he 7 nf” Cy" Wd vk “y 89 +4 Jo + ty ++ Ge. 34. RIGA pw. Tor EMO. ( Hlgy Tabretoas by f bias C~-l Fermentation “nutants 75 Jan 5, 1948. 3P. Inoc. Y105 into YP broth to obtain calls. —f ee eer Ure Ybuep " Quadeabs ey Cy en ifn op Oy Has at Le 2. Nw ENB 37pldis * e090 [pate . = 700 colnrers . ; Mo vexxr tamHe Leet 1h 39 x ta 26 = 7232 tlreees | | Jae eho ual steale nef ny Yo! and! Late. MNol—- on. Lact vad wiooplerleahle Aiviletal vl, Has "ypeer. 1. Re 36 4s 5. 6. 7. Nutrition of #93 Jane 9 1947 Inoc from fresh slant into: T(Val) plus: UPA. HC + Vits ~ HCVits ++ NZVits hep HCOV~ YNA _ XY. “xtr $td+ Sodichwaex 75 Looks fv sp, women Ay - Kell Fd darpenrenes 7% fatiat oSayk “ Tlbeca) > bcadeale foster at | o 3 /) / apeckie Q 7 Gung) wll 20 16 ¢ Ww f Ly é 7 30 (o “ x O 60 oO GO OQ J20 a [fo O. i/9f7 PH. Pepead. poms 20 5 Yio Mufeliny Kern. 77 ye 84d 7. an TS. fade Yro Y 1 36 yx Lop = 7,97 ¢f,, ebfras ploter oe w/e fp © tend - Peibiele © peng 903 7 Q pal- Foye} 2 a) Le , 4+ ALC . PrekeqonW75 3 @ tad-. Fae. - - g 4 G@ + ad ; oy Sy + @® +t+ad- Retualn <0 pct 05 te C @ taf- ——— 7+ oe 36 plakor x = Tor els 1 @ +uds- W10g - 00/04 2. @ Reeder 2. @) + amd + (@). WOK Cdt{ I +4. See 197, 1 | ar S. @ 4 rag Cd 112 = ctf > +e Utilization tests 80 Jan 9, 1948. Lactose analogues 1% EMB b-Me-galact b-N-butyl gala. 0-Cresyl-—b-galac - Y1O —_ Lat +h te aie bua, % 153 bey th a O phedf ¥35 bac, - —_ — - Phraagee nailed YAS Lee, - — — — Heldrastactok, rolls tburrced be ef loping 2 jah raffacet| Futre Huh (Mol YYo lu ~| ree vbross-*est Lactose ~wutants &] January 4, 194%. Inoculate YP broths with foilowing: Y¥53 (Lacy~) and: Cross each on three plates. A&.( fopotrns +) ao W~-30 . mm 7 2 #35 +t 3 . 3 ee A Lileg 635. Ca Sito. “4 4a = A@® -— pifriot utd! a) W-43 ‘hoo + LihebS. Chor < ilo. ; sinehd 7 W-45 t+ Yr - "a. Ses ¢! 8--———— W477 9 W-48 ‘lou + 10 +65 - AL - Dit eelay 10 fs 160. darvest and mix cells. Plate dilute on Es—Lac(B)). oe Vive pwn te be elleta uth $2. lac,- - a) W3s, wus ot lac theontoraty. b) Wye, Wr, VHS WHS JbS.- ta. (%le bact Kecontuute . c) Y 53. (8 7 >) . deyrreal date ai VS) ipcce Eta e lite a bene Streaben tA eth be - aud Mal— wachenils frvneebectel sial tose segregation: Effect of preadaptation on maltose. FD “anuary 8, 1948 .Prepare inocula overnight in YP broth, Y40 10 AM add 2-3 ml to YP-maltosa (4,B) and YP-glucose (C,D) broths. Incubate W-1 similarly in YP for five hours to 2 PM Cultures of Y-40O are actively producing gas at this time. as and cross samples of A,B,C,D, with W-1. Plate on synthetic EM-Maltose(B, ). Sela wrt. Me OMe A 5, Count -“ A. (112) 4 «30 ° Z 768 eo BZ ag o 6 13 / A 156 S 9 24 e O 5 eS y> / 2. CY ! __ yg pt 2. 3.099 "Jo B 6 6& Oo /7 2 /2- OT Zz ne {UX 2FY UW 1 (0 / JO AST za 12> . | Yb 2023 2 74 2.28%, Gj g - wag ef fi 3y8o 357) 2.548 Po /} | Cs (cr) N- M+. S / 3 Aol 2. 2 47 eS 3. a JOG 2 yg OS ‘. : 26 7 b. L sa l ?, 2 72 / g, © 7T/ © 4. Ze /0/9. wal: sodf D: [@2 I, 26F) ) 3 2/3 3 3 fos / / 357 2 a 16s7 1S 2 ae ee u | JP WD 3, 63 213) 2/94, vA 2 PTI VMNL +. Con foacan, 7 . | ! 4 | cae" 3s | | 3806 Auteead , 6S 213) 2194, —— ! om fof | boyd 41 3¥8O' 357 L Fel te »—~ 154 sert) 5 765 —— - § tL ~ Nem: Mrhr ~ SIS 021 % cae S24. Y1LO5 Mutation run: Lac attempts Jan 12, 1948 Irradiate .1 ml per plate (ffacKMB) 9 secs. under Hanovia. 71 plates x ca. 30 colonies or 2000 colonies. 3 suspicious colonies streaked out: l: so Gross-test Y1O— mutants & SS Jan. 13, 1948. Plate mixtures on Lac tose-EuisB; : Y87 (Lac4—) W-45 (Lacy~) eo & . 153 O18) We, et feel as wpheiaLen wa W108 We pe lac, - W-112 a VAS eet we Un Mal tose EMSBy 3 a b W56 (Mal, —) W-60 (ifal 5) W-1 (rie) tre Hely-; Wl w-se. W95 aos. ++ Naly - 1: W-6o W-%6 yt ++" NaQy ~ all thurs. 98 = (i:o00) Hb WOO = - av (in02 “ th M104, = ++ W106 ~ et “Gebel. +t > [:}0e He f:/o Ht = magna taeraal coy ys ~P 178 Sourbaak rr illalim 38 a Wo ' Mo p | wile 2. WO oper 14 Dagalicn wse = Ua. oe vow oe. Jo} sown pin WIS f ; | wth p ; vfow Ep Polysaccharide Utilization Jan 10 ff 1948 Test strains indicated on T(m) plus .05% substrate. As Enulin . We55 Apf (39) P12 (48h) - - —_—~— B. "Bacterial Dextran" = Lot L-10 from fire = K.P.Link — ~— Inoe. P12 C. "Soluble Starch" as above, Ai +. ++ 2 hechidt cbr Aly + tte ALS WS 5 hone semen aceinun lati a id Canes bon’ arn Area lagerTlio aor patabls sec pad clogs aarey ae crngolitel, . le Saxthenret “ Gao 7??? | wy Be. bee - Caos Jeoke. o” Cpe ratty B-/4- La - nerfed ankle LA, fee, Aare bee x Wily. teabivr , YS3 aud W-/oF ho 2. LOAM Ler-/0 8, ye70 WI ncn: et reccet Ww 5 aan. dH O- + Wy r: ve A+ wf. te wy 5Y mt. Le ey ld 5S + ghee nibicrudutin ?? Wb 7 a Aaa? nic OE, tern (pom pe) td Pw nic Bettas Het mer LW) 74 +H the W76 OS $y en Wd3 ++ me HE WET are He Vio-Lac NN hebor, Por, . LP 1G, (148. Sr abet all ages 0.109 07 100 (oh) ores Pee (Fac na Safe Hh mp acorges . ne IRL WI4 yy W2Gre0e. [% , dlatewe he (abr sep.) av Ky HPoy + Rt, Poy (3:1) 7 Popa. YL. at 37° wth wercton | jeer "po 7 Colt S34. pac im Hacples Resscapind im fYorh 3 hekao Mat parle with peaks, SAN etal pes wmf . . f ~. Lhe MWe shel wall ,¢ erocbne RH Buia | Rerpend oy WP ee r-teale Bln tacks) Sclhemainuh glean ¢ babes inde coll ae TT tO abs, elerhel achat mm npugeralen. To bec pon (WO To armmarndlr , ( YOud. ) tho (au. Marefonm & Yor. Viorel. ty,0H 2 UW ond? shng, PiPDPeeant nelle fant. Sehimunhk sh utesrole m Sornt Iwo vols 95% alert ma fecte fast. Peporat —» 2. auc. abe. mere. peck | Y IZ . (b. amie CHD a-' Pr0W- pestionn . Sedernt areel hecacct- wd phat CH, "ale TR So She bbe yoybad Nw PG cblol muta |om A wm 2ome Mth. WlSul ao elt tho Led all le taste b tack, (122 fn BY), Wr te clan |, Sthuemindh avid hiflol unl, CU ie / um vod pao webre Sof OH" FOAK fone CH Uy aed’ ppt ct Ts Nad nd aud wer, unt, 9S Ye ake. Ln CHEE» | 0 wk Ho auth Sx ehetol. Nyt prone of yeu ne IH te Ro hia det othr. Uh. m Onrl Ate sel" i, S ves. haber stand aeecerpand wm hee MQ, 10 mb. eran Note bcd ne th duels cherhof Ms lbh =) | 19,1948 OMIA t ( CL Indl. fod eB. neg. EC foul YR beth files /stea.)- Lee 7 sLiadh fats areehile tasty of Mis cl. ies wit (a 1138. " Add, Bh C see for tio tualont contacts. fd 4 20. Ay ate Tek . ) , L A ate t. A plage 3 n> . 200 Nak > agp | a 4 Af : done eT hmty Ww. ? A ot ; Dy —eeteeces- came vig Ferny Not cats b BY AL Nel +. 7 Le ts | & B?> eee" ; q Bst Tubd) ob. aut Conf, Not eet 0 Bot. ae whe. nD, =" e telmaces I C! ALE Mak + Iv CTL i . 13 C3. => =~ ™ oe 4 v — u- > 17H 137) G2 O A of | o oo : Oo 7 C S 0 PG Dy oO O 34 #O 3 oO O oO 3 gO OO Pe 4s Oo oO ] 6 Oo oO ( oO oO Oo O I 32 oO © 6 hh elle ry ores | tT we to gh a ea ; } 4 w' A Qo, + L the Hw or KS , of iP. gf Prrggaratirg of WP on 1998. i) Yel) : ‘Ty peste nd fo Vom ck lacho! lad a ¢ the iy 70%, chested / ‘c) specific cevtenara fred. aS fen? /9¥ 8 Peele Me floons inet Kove oy Ue echo, wrbial fp) & Fama /elucoae, Me palin 4 19- — — © below (allan $787 —— . Jaa 31 hussain” me gheee pil ne » hacker and on pwlbleic | Ap Bl genre success ue le Jacki 6 wlleie +. folo MH rapier Fran pee tNol EMA. Schendd Gb MeL - Mars. + Man. LM, — Warr MAt ancl lacna, lolfolt act Lact ’ 3 n Me: ; wo s how 6 lactrarr Malt. frrfys Reman & Y/0, Fon 4q> aut 08m /M é LL oa. pe ey af Fan q> Tim). Melblae ok H (Ta) to [eee Me. od byes 3 Talore 6 Sebut. ane a0 w-0e ® = WI/6, Ulleae 13 Viaruntof . of Sy tab albpetlin Charakinplon of W108. AG ty 0 _ VE ed. TER, tla) +: or ¥ 2 (tubo Dgethe ). Wik Y¥/o. yous - t+ v-hiloglurence — + hateae EOrpleap bite ++ ++. + yncone +t . The MDP aren aeiptacad from the le sett proluct agecan atalikee od waacbralin othe ‘hen In, elution amr whieh, <1 carduthy ait vilulrley hens the wonliol. dyectrlerng | Hoa Rpt teben TM ap, — teb- 2 - x 5. peettapha A tM 2, - he Dit Meth= Tage ~. ar anebnisre moran) rude | Juez. ag. + tale nto VP path. Os GEs37 Asivszy (A) ma Veal cyte tangles of [ele cables mr acobrnase Thm) tmisanal (. of {2 ca, Y, SI4837. | ewecks eps aca b- 93 337 ta, 16-2ecels. . 5 “ bat iwof &. De. ay) SF harry oviell, | Sas! } / 2. $37 0 ,O 3 O, Doon. cok lO cab. 2 0 UY St 4537 — Dey yo-We., sO0t+, /60C. oe ll. / / y Aaa [oD “ 2 ZL ° b vr, cn, oA " . 7? Vite. Tet” 4b wrod: ae ee ee an + tulelee ENB /. Aare Or | i all i All, . : a fof 1. Tipe hy T(n,), faeth, poop 1 mylene pet a ally mr gframmat bchaless, ft te vale fom Dhricors Pale eoae, Toba 19-1 paper» a WH NT W 117 co blr an aoretrer opedletes om 1 elec 2 Hour fumed. m gfucone anol n, K glenn + reat, ©, tae Fees coloaian fone frat 117 Wer?: Panree fede | 102 en a eae pnd ty enk iataat fo abtphonel © teahtrout Sake out, a Lba. 108, Yio — y oR. ~ ts gee ¢ “ ee emia SJreabeoat ®¥—> Lha- ant lbat per. Prerdigand) denube a2 Our Teator, lactece : Teen wall pe tae w bba- . y/o _ MH Mebrrouse Mell: Fld mat ier wos - Mummers aval poplar (9-6 [cxtoes) Our preonrk deny, He onal papill, raehizabed on lho bell itterrers, 4 Arh LD cates Urcsg Oy lecfors. LUOHIS ze + Arf, Nia prok pape. tbl, aye gull Loa - «| Wha me Leg? ? ragelot ve ot ) PR Oe te Lm ieee th perathr Yio cott + t and — TI 5 ty - 134 16V 16) dO It / hy ys sf of 66 G6] erlorices - a, lee Mutton, Kun. 03 jer ( tt& Feb ve Y/0. (Sv peta xX cao? Swe cobseia adelle pun plate anaer = (Ca. /02, 970 Crloeces Moat uunctawke ae bch clr ra ther flac, sects . Stabs ot m ENMLa«ace- < hiatal (0" bbs plete _ Atte . unku lih,,'s Mow pssrrins anions prurtendee 1-39 kata t utile choneas. Tat iter purter: Nel fore Mlucore fag | tJ - Neltore Bese TI Ww - f= Ae IY6 Br. + t 135° 2- + + + Wwe 32. + + 136 _ 7 7 4 33. f+ * + 121 oy +t 134 5. Tt 148 25 - - - )60 G. t + i I~2R #. - - 16f 27 ~ fe 14 37. r + 128 &. x x fem 123 38. - - (6 4. 4+ 1 34. - - 163 lo. + + BL- 11S [ Sechorted:— hom ~ t+ 152 - 1.007 ~ dap- r>{ Yo, 3, + + fe 1% iY. ~ /7v 5-0 7 - Rals 15> Wi. (6, ~ ” 4 154 17. ¥ + de 1-7 (3. + ' 18 ¥). 4 we So a las UO ee 129 43. ral + F a —N po 23 + + - Ids wl, to ~~ nr | yy. wer + + ty oN IDD -~ tft = mi - 4 ~> WM YS. I t : aN et up oR Iv 394: moe Qa f 1 0) 5 goo to Par falas +f AVY ~ the + + y; eR. tor F r = Dna. (glace a) Mabelics’ Keven. Joy FL .3 1948 A). Jo" ells en plolt Smns.unda b4tenn’s Slap 6 a Joo > BIO ceborree?. p). 108k. 73 Seca. Hs dang ta ‘ty Lrnrrradabl . 40 petro x Fao = 20, 070. No vee cheeccut Colones gr tector, Sfrrebe out” Cuspecerus tolonicis Howe on yecenee feos A. (otnd colony) W16G. 2. PRurcone — fom ” as Wlfo \ © ee ple plc Mans bade bere. Auber. TH WY. oe Rr Ke Ke gg 5 yar | WIfo + vs. ++ ++. Vem. + ° t+ +4 S Witt te +e t+ a S WITL, th bade te te La S (Aseiing) WIE? oo bryorr Hore a . WITh meee nee Mallory — Ttaledoar + ? WwidyS, ++. tm “ S WNT x VHC JO Wok —r2evrtcreen oe peeett teccketinn E.b.L, 199. Ry EMS - Cone. . Coan WIT (wu ues partial pivecroon ) x YO [url farted). ral i flames Fb .5, 1998. Bhat casihy der teriguialieh fmm Aerchece f Maer - ont, Terodarres of LH caumet bebingucsted on Ue ot I apat ign Yond bok pale oF ngrt Yla- ny EMD aad conpa nll Gand WIT. bn at Ala =. ‘ rami R © ++ 7 184, «Bas 3S Ma core ++ Ve ov? Muss vi" ys 13 S| f oO s&s F 16 3 /6 Lf S / ly a /f 4 /x. ss 1G = —_— 58 23 - SO is flaraer errudalh, auvicler C ftavent (Hel, (wt). ponerse anh Dut are Diff bist mgeusals . ca. Avan urlnrs, pocls the and dial _ tae wl trypace ales uth wt are Y/0: UT Tepe ti aba tpi of 2 “Ete (over) Y/0 worl WIT arf LU 0F mr ghescoee EVID 2¥L,. Yéh - Vio HE - Wit? — + Wwio & a — | _ _ a _ _ jo6 Fr. 1448. nit SP-161 my Ay ENG plea es on eelof v3 = pease at h geeeew S. Teler tr” and Sg : is oben. 6 : or w o1Ty ° ud ~ - - 176,177 oo ee heb. 5, 1478. Y/0. 50 plalis X ca. 150 geocenble toteuts — > 750 Colrrer, Beane colorers cratered a gel én. /, (@) tanl — w- /&oO 2, he. t and — w- 18]. 3, © - Mutat, Aun. (O¥ Feb. 6,998 , ? SP-161 (Sandh) nbeale (0 cella |ylale CI, m lac ENE. 7S pate x BOO Gueeenrr = Ca. 22, 0. pemerble colons hh. PT + hut nf. Potlranns ee turnta pt harrece' A) - $6 Vbd lobe I. 182 - 2. 183 7 3, 184 - | 4. 185 - Sitref oF. EE [&6 - Citcad 6. (@) (87 Hos — Heim Pheer. 7, #) 38. ~ 8.0 154 oer apo ‘fee Mek Ae the Ana Kok fre Pv 193 18y es 186 187 18x ) 94 Falrb 1978. X. W-|Lo yl Vee 1-3 vy Vy I 127 U7 19 l3f 3 13> Loar (at Loe Muti, (09 A W-Y¥S ff tH + Pe tho er br tH +) B YOT / aXe hal, + (1/300) oN Cath, plate had A — Lae,- — Laget St0-2e0ecble totosa ulnar pee — (K tow tole.) bae.- ¥ OK Lae, - + (1+ [yo00) * © o% - he ~ Lee, - _ lee, — 7 lec, - — lac, - ~ lee, - (Stark | [poem + Hon — (How?) Let,- _ Lie , _ Lee , + (1]10) ee < Ga n> . Aa Ag oe ' t tebe recK Hts i gaat CLBAD. free liyeics hWnd Ifo Ptr. b, /9 Ee, Wit aa bac~ Mel— Bhat. lors unth Wi 997 trayelil, lela, aud ut (Wo) Hie bac -Mel-~ tle. Cogann, Lac ardlla, Loot Wildy x YE7 > tt lec. Veto els. WI4 Sx WHS D> Pe edaeen (Had ). We xX YY¥o a, ieatteee > breary gaits 7 vadbogrond 5 maymtcens (flr dey bent Jo .Lae. tdoccapl ° Pi cle fron bees te Taf EMS + vee purg, Lace Tteililm Maks | Melt Meal- a O 42-6 hrsectactead . Halt Lolo bees + 7 (OA si" 20b A/c Recmbnents forende vm wart, bae= war ceparalid rom Kal —. W—It7 [Wi0oe. ne Pb. L878. A. Or, Shereore EMS, Wioe«x YYO p. Wu? xV¥Yo. C.LEL8) WHEY VY¥O. Ol, craeren gure laa ee avd Aly". Abi asa ute The “~" coknuis vn Bans clase. Tham vir A » eg ane not waddy onthes phil Prete” telmur ot arcelom rover hand BP anal slirale rtm fol, ENB. A Ke - (17) ©. Mee (24). + ofl Magy Ch. ft 94h ( Cm) + Os Pror WwW 7 pq 1. Dlirear At pid at 2. baclve ++ * Har 3B. Nelfru ae Kk ea OF Y. Pramerm Acct fee te S. Seucenre. _ _ ¢ _ i 1 Rall ment - oo gO" - ~ 1 Cbletrore +. + JO.dMe glue _ _ |! Lectoteonsk Pagina [Pneclon Sebianiect , - * Shale ved . ee a I, Au - ttt and + L. tele? >. tod = + ane - * ba : Nal jhe, ; si Mh -a~E PU + Cu7h pe) }. ++ and ~ H oud — He wh tng (Mdtosneat 48h.) 3. t+ ard - Hw ond - ae. frogs Z++ 2a W- (set) ek een aa 2 watacligrade im hecy Ein belie, bnmenererti well Vy rig stra Tinga siffenlal at Tips Bat ee Bett Aiet 2 Melt and Wlaer " L tres tated mn ghescore Ale tee. feot Lae + pad aud du: “eas 16 19YP, Fame Wa A elitr, beet etme ure Tisladlre MeL and M70 /lae of 30 Leet telomesr [Lo une Mal +. ae ae Mek + ) GF ure bee -. 1o- 20, Miche dl pron oe lac + Mal. Feat ccadteng's 24 by, Lac Nad ( + ~ 2. + - @ + = lu—- 26 ¥ + - 5 + - 6 + ~ 7 r — ree 8 + slur — + + - {0 + ~_ " + _ IL + 7 > - - iv - - if, - - K - - 17 ~ - 1S 7 - 19 - ~ }12 8. Frau 19, 1908. PIP he Wok hawrley eae te T Cay) 4. A.low 8. Hef. P19. baw t+t “ 1a - ret Hraleadt bac av be anced bole fore pcre cesrterincs po ML ifr TP LAL bats m: Hyttorr Bly rorr Thee Acorns? Le Br on 8S - heer Mak- Ah, - / + Or, . Mert of He Mal - fost, jamaplert Set 2 ad ake d He Ce nerohs . b “ : -. hee . l. Sse fs = 244, Yo pinks, feb lu- WB. + = Wid —_ —_ wit 7 +h — hte = 244. fein raved ota 64 bee thd ~ dirmcal wacky lpg malic nf, _. hte tb Vonks ao W-251 aud Y-~2FL Meltoat Bicone prateee Mohr ae: — it J- 65+ 2 a Cate Me erty gerap ro tae: + Yr. ‘ + + - - _ W-327 Mth-Le 5 + +t = = + { + + - = + S - = + + G6 + + - 5 t + W-328 Me GuoL+ 7, + + = at S * 4F -— 7 too FL 2,19 78. lac feet -ceoates, heels, Fly 129d YG] (Lec, -) x Lex + bacw / w -/0 } ltoo © CH / / 020 oO / Ov~w } JOD . A, Welty. O 2. ¢ q I pn ! 2 > /2?. 19 3 lo-/%4 O 30 QO 30 ° So 2 Sov /0@ Is on ttYS- bere W/r0 Vt Lac, _ wes Wot bac. WS wo, WIV Wot Lar, - W/o. W-17o. W bop se & + ™~ & Ww -{[56. Phe peatly YS> — | 0 OD A we » ba lee C _ Jecks 6 Feb 12, 1498 4, EMS- Lae. WHIT x wo wlyS x Y€7. (lee 2 pote). wW-/4¥f~ x YYo., Ww-ILG Kx wes W-INF « VST. bac+ bee- - ioe ~ a2) ar . - Jos -. WINS <2 (lac, -)- Mot pobiabpc = pe ardrongace nth, YS3. 152. O Retanbrske 142750 Veokt . YOD 140 a OC. 2Y¥Go @ mL 7732 - “1 plctre Vo cloreds / é 1 Mlaenall Ban ehh mudelin Fr: 28% - ae = 3 *h tact AL conrtrranite . thot D plac. No telonrrces . [What in vrcomg with WS? 1 fel JL ( | Yoglecere- Pe Mtb Rel Ju fra (ut ha, faba (1, Baf. Stoo (1 209° + ¢¢#t + ttt t ft tt - ~ YgO Ft ttt +t at aa — — —~ fest 1? (17?) | MS | “fr > é | ( Bde /) 8 yf ae w- bee Hel fol leon Xalpee — Mecbanerse Hebllf Pe bc 7 JOR ~ OO he re +t + + ott [e + 1 2 )8> ~ eye 4e ae + 2 ee tH +e “$e “ 3 SY ~ “+t tT i+ © er r « fo wd t+ , yo FS - em ee meer te ee 5S Le HHO Ke FG ~ + ods ~+, 3 bt ye bye) of ee . 187 en ee as + Toe ae 7 - Bem mre - 7 - oe woes - # oa ~ oe (- - ee at - (=) _ ~~ ——, 4} - : - — he aget 4 J08. © me wm ne te 0 TY pt Ee et a t pH vr +R eo WS 2 +r -~ + a br Rot L. —~—-+t - bon yee — 77 os + - + + t+ rode + - 44 we pee - Jd x Hep Ne mm tas =, ar a : a oR eS if pp > 7 jee ae 7 ae dee a ae 1y ways. ~ mo CG + hee = ee ars “ 16 6 f+ ++ $4 + PE bot pte wy WA “7 we ee + ~ e+ ! [fo -~ be ro bot Ww - ew 4 ete 4 14 18 | Fe ee fF eR he Te pe ye — 20. (DQ, Te or 4 + a —~ oe - oh. "2 So aH thot be +- -- nr - cf Le />6 - mm hy ++ gt eo + -m (Sr 6 2B 100 0 = 7 = 4+ bo! bee da y 133 0 < - - ++ wr am- H- --) \ 25 Io - ui Ate ao “ wor ne % 56 7 ss ++ 44 ¥ roe a “ 37 VI ae 44 fee ; ie ke Lf Ye 123 “ RY + 44 oe : ' qt - ia ~ Wm )2 ~ 7 We ye ya 7 v - oe te ee . do by - j \e ote de ~ t , - + (BA muda K W-}16. ELV 978. Quy Lae EMS W-126 x v9) /}, 35° tH V4 roe poe . + roe, oso. 2 Ys * 4 ys $+ — YE Sse thee 5 65° a/ Ud oe t 6 6] Wow ° /be0 7 Fb +o ¢r 8 74 Yan, ew + 4 78 y 2) Fev t 16 83 ‘/ 50, soe if wel 7 (b00' boo. +. (bem: 5/30 + 383 ¥ 00; = /eo0 + 186 400 , Yyro + Nn. (f+ meme YS3 “go ofr | +? Ib-18 > x YS 0/6; we 0/S00 186% ys o/gon Yeon * H loads et. wo dy. 18 2B. w-3e “Wed te ans w 40, 65 71, 76,87 ellipses WG [eatin ENG +t oY. —_Y¥o-—av 24 eco Far 74 V7 $4 + 5 _ Auk Dna fame Ne bal by bal we fOF — +4 FO tt + H+ wT + ++ tft 4b, tte wit ++ Ht een ~ _ WIP +4 HE +44- _ - We he ete A pecudiar | BW Shek nt W108 mr ghucore ancl ya lecfore Mu, Ah - Tarot [eons D Pauls steowg ak bal Repeat 215 vite. Palle begs Mg W108. “lathe Caro cates tps oe Yrola Dlu- Salt | alin /y 8. Yai Sag W-K MEMS. atm wi bail +++ © © Chacads _ Lan fa Ba dna bow Hed be4-— ++ 190 rte ¢t vt —~ San ae its —. +¢ u ~ - Fr + ++ +4 - t+ 1973 rt +t oo _ ++ a +e +t +t ~ +r 19S - +o we ~ 7 M40 +r et +4 - ++ iv ee ++ “A _ H+ / ff te ott UA + +t 179 + 00 He +t ++ 72 = th Laan as te LoL +t th He 26> _ + 4 ~ ad voy - + - - - lor ++ +t ut _ 4+ ws So 4 ~ ++ 207 - t 4 _ — 20¢ ++ rT ++ ~ +4 209 ++ +t we - +t 240 te ete v- eae. a4 Yr +t ++ _ + oe +t 4 H+ _ +t — t _ _ 3 + +t t a + MS ga Vee we et 4 ++ u7 Ve ttt +4 _ oe 248 we a _ +4? 2/4 _ t ++ ~ —_ rte a = To 122 + wt Le — te 223 H+ at St — eo 22 %_ _ + ve —_ - 229 + Lt + _ $+ 226 - + v4 — = 22-7 - r we 7 ~ 228 H+ de V. 7 Lae 224 - wh oad + agar a ir | pottype of ipe 10? fre [OF soe 10 flips lof “qpe Ft ot pe. fod ope Fiebre 16 98 A.w-4) 1 1976 + (72 > 493 « (94 I tte 6 177 >» Lor 4 203 10 20 6 " 202 i, 209 > Ztf if 2) 2. 1s Zire % us. '7 LI6é 9 Ln? "4 clr ° 221 hi 222 w~ 223 > 22357 1f 227 KX we W188} W908, J Suit Hy ogee Ped yg: ACL fae, > We yay Vk to? tHe armenia, martin teow, dol. ‘am fod ad taints tg eat Wail ao Hegelat CMB af-3/va, a B. YO? x we B ¢] 100 t+/l rot. i ofrev 0 free 6/7 eo N)00 ofjge 0fj00 of ig of bce , 0/100 * oo *|se a so lie yl ° frase 0/ 2eo 0/300 9] 30D 3/34 "he + of sum iPro 0 {10D afro ]so0 o/son ©/700 ofse efjoo of 2m Wb S <> leg - +>! Dw LAt, ~ LAC, - LAC, - LAC, 7 tay LAC \- LAC,* At, - lac, ” LAC,~ LAC, - LAC, ~ LAL, - LkC,- FAC, — Lat, — Ma, 1797, 201, 27%, 214, 215,917, 2418, eae, 24 218 Banks Pre patel (chy ta 123 Lac Mutants Yross-Tests Febr. 16, 1948. Cross on EMS-Lac B). A xX W-45 B x Y~87 C x W-67 (Lac) Lacy Lac, 1 A , B . Cc 2 — a) tow o of, / 120 pee /?00 26% [V0 Aye a 122 tthe “ s Vouro 0/00 o/ a0 ww 125 Hew ++ ee bac 6 of 132 te ofgo0 «/4o0 ‘how Yfpny NUL. bac, andbeey ~ re ete as aly "atte, on Ley iyo me */1@ Ara” 500 yin Colt. Po é 15 tor th +t Pints, BY cae nth fem Gone eect th : f, 132 awd 140 path, uo Lac + ef c Lac, on t beey 14% Gene tent = both . Qo 4 IRD ae becy Febag! 48 58-161 > -C-f. ~ ‘y 75 plik Kea. 20 (Lune ) * 17,003 Celoreed | Blkn Arak tec Rekerd on EMA Saker. 4-237. © t+ be - Ng. ~” W-238 @ +t $+ _ 234 O $- WGa. - -2Y0 “ +r t+ - Lf / 4 tt ++ - Porer + wy +t *t + v3 ‘ aw t+ - yy ' +t te ¢4 _ oe i @ - - f= rV¥6 " +t ¢¢ t+ ~ 247" tH4e- +4 —_ a) UF _ V9 _ 56 ~ lapis tee 237,238 L¥0241 2VY 2% 2x7 Pha - 239, LY> 2S. Seo flr Mell +> + + + +S $ttTFETEFR S FZ y > ++ + + t+, ++ J “/ bac, Aen aBlolae hanger ~ eg" le, HVE bial rt wo -18 ee a lab wd mre iactere Le ae ; Ws bo aw opal Pra De, Meany woe! vite Thm) + Lec + Puget rho ~ _ P22 — W-/Was Coneplitly ohh 2) Srubad W-1384, Ghbe + Conan nk tt YET, a | oY . Ale - pple L Eaten saga W138 < our + (0 os pagillte ‘ Aw — Vet ow + Upp Ot | Art _ Sly + ! hoctirals . Chybqomee Nha /26. a xfs /V8. Hebe vp bh la (seers reals) an 2% og. Arenay Mbit 1 Mya A.- BMA 2% Svobewir, SuecectnLe, Mfc anclorleirns , A ashlee: bs I gl of WP. ive W- 236 x Y40. Gy EMS. G5 10% bas There, Wer 26s rot beer+, Cle the qtr "On UN 0 — WI T Styt (Ss proeen bac ) Call tu. Ip tosidan 470 aud-r36 Slat. Mh t Sat Peer A-wi0% x B= FS A (W229 tt 2, W2Y3 ++, Men t wile (er caver pte. 6 tench | 3. W2rYS. fof lo€ R/S vacesti', 1 /> x + ster 1s; eu | aan Wikis rwlmg — Aeclanmgarmrnel + CY> A= OR, Than’ colonies? 245 50% peel. | Cprees mtacedinne / y Cte, —2y¥3ne? ADgk, [afetleag Man. 30 Se -/6 | SO Ren. Lt) kan, ‘g kM 5? pickin x Do = 10 gov Ato) ( W-as2 Bot 10 flan perched wret ood puch Poke Oo glacorale beat , 74 x v C y A ‘ + > x »1bi.tH “3! fa, V4 rA+wtt +t ppt) 1% ? \ teat ese eLetter t rere eete iy ! a l pert le rar ge BTR! Hwa * | + ]) Leer Pee Ltt eee Tae tts \ LTT [?. beeqee tet pee etaatine fH THA HE ee te pe ttt treet et 2 Tie Eg te eee aa eet ee EEE EEE EL THEP TET He ee gen an eae Hee bee 108 8 Lac lac Lac lak = at+tt1 + Pe 108 108 lof 108 Pitt tp ett tet (iys ~ t teat ll ttagtert 4a dee, re ¢ ee pas fe SPR s 4 amewr re falters | I ~ + + it + + + + + +s + + ro y + ¥ + i 2 - ~ 3 + + Y - ’ _ Sor ja 7 6 +47 \¢7 7 + + 6 - - Poi - 10 i+ + A Lacteee , Valatere ~ + - + - + - t — t - + — t - + - + - r 7 + - + - tr, lege. | + ~~ + —_ + v _. wv + 4~ — x. -_ , +. oar + x ~~ ae er et Tee rH He + NVA +r re ttee ert /32A. Hegek ptt tt + ++ + (33 Copan ~ Terk Lae J/>> , *# beey a W nyf, FHS bac F elates cach. A. YS% x YST. o/yeo of ym |r 600 pery~ G-N- aXe of 4am of ree fou La aes bor afro [veo afer fp. YS 6 Ww 67 ‘seo, Sreo °ls 0 °/5 56 a / ber becu- fran S10 ee ‘rw. c. wiré * ws7 Sen Se hoo Srev 0/240 L- bee, lye yon ofre, °/d00 D whe x WwW 67 foo o/c, fe, 2/re the off 300 Lary? Sram efron S/re> ¢ fire E w{ro x Ye? f Ye, ‘rue, ofyeo o/ste , 3/32 Le y hee - o/ sa °F apres 2/Ber o)sr F Wiro x W67 ‘Iye of yer , of | [rr 0/200) Fey Letyn °/ Bex e/ /rew ofrow of Y53 Ay 00. +: Lac, 53,87 (wns WT Ys? Lacy ws7 W Ire, (nd) Wwf{ye WIS WILF ray eo A fewnueg Li bbl hrei, 01 « doaele wascbauh. | dye haart Liicke Toy) 1 4 MA dele. aling 38 26 + + 24 aah 219 4- re I9r + Yo 222 + 7 223 + {2 227 + 4> 2728 + E. 23, Wok kr» a LeMay E/11B. se/ple 1357 Clitch, ober . Eb. 8 Steals onT WA sforlrr om atu 4 Lae EHP: hae Phe. W- (09 cee- ‘ 188 B= 1+ | 02 - b>) pap. 239 chase dais, meer v. wnvell lpuee . (beset ?) 143 tt all t. LYS ie barge +, sual - elf, 2 cela Sarge 254 t++ “fy. 252 t+ wee lo coal, 327 HH omy att — 8 i GE Heed fr be ape pi off ?? Fhrinny LYK (7% [. W-35. Resusrhh $5- at Sy -b ; ALT on dctose , Hegel 53a oy Rabe i 556 A * 136. 2. Toot W853 papethe from pl, gel + 2a om af fleas wala we = T/. ay few fe ile Ton me ROvre. tee w. 4S FL, . P24. lo. v mt MP i tt uct, lee Ma. o:2 _ — +t : "> eathyett Meath, + W2eT Co 197 +4 * Ht 4 He lle Hub a ff atl +, Mp, be. . w-tv ‘* | v 1. Tent WIS /ban papitles on Hegel G+. O- 2.) BT [2S bacth: bet mn Negal. Ault. “ (€ Ne say % teate cla, bag ACP + ! (2 Lact® " " W1+gak tr. 3> wits Banat” on bax Atl + 9 " " 7%ak as IT WI88 lus my Lae AE Ur 4-24 Lae pn fr CA + 5D Maks 1m ef One Scecewae . "teffed Rereimenicn, 36a Feb. 15 1498. Pape, —, pul. sre Na C0 $0 Wy Ho r KH, Poy nk aufotace lt >F fasta Ree KID Ad. Secaptrcaim ate : p25. A. — B. WHyR Sto (2%) %Oce ¢. Unum 20%, shee fAr (oi) See D. Bowes (5) (5%) LS ee E. Aappaagenne, 5. (2%) 2.0¢e Find aduthem’ x9 va. IS N. ID7. Ss d 3 ' ArT. P24 V dncee + ttt +++ 4 ck ~ ++ +t . +++ +4-4-4+-+ ! ~ marie ! Cor leat bac Mufaula. shay 133 Repercr. E, 27,998. = w-4S br lac- ~ - O fag ger frteecd rhe frugal ~ ect the ect bre ~ sores +76 +44 oo Mes aeee vg ties ace. Mea 286 +4 e ome 287 +t ow 313 te “me 316 +e 317 tp ee (2) Test 327 ¢324 whe Yiot W136 yy bee Hh fr Sappesatees 327 x Y/0 on mols Satliclic S plete . No cotlanen bac EMP" | pate We celauen Tos tof Boy a re os. 3 324236. (W-35 bac + Ker My - x ws My elees~). anaes tH, - 7. 324 Vi0m Lac. Heppaceatiy all +, So pS sd = 15% ‘eee had TP etee, Hiecce aw af head Ballhes at the Lacy Ares: » Hdd > baet/ [ Tet zts pe meh t - + hu halted panne | Tent pritclioplir om Ma S. Thethe ! Slerh haus ber, cg. P% — 2. er I 3S Fb. 25,1998. " Cnofank” gtowcatam fon Pilea, fucbriony curadecleg : |Log bubble [iif A ple YOaecr. T FO Mees. T [0 Ares ta [ T22 134 CLs ave. | score - ~E A W108 xX W-18e. Yel pug Ge, oe ofS og Tia: 0 fH]. 6/4 of, W108 wd, 0/13 of? ocho Mr O/re of 7. iy ae Coors tok be Tput foe Ww-L3% - of sen 0/260 °/ om o/ foo . 1? Shi heret. w- 232. ofroo of of wo 4/300. WW- 234. Oo beat boll Y 1680 aa W-L>1 — °/ 245 [300 © /roo 2? S.0. W-3d, of 12 fv ree +/r0m — W34 of i509, of dow 0/00. Tob ly- baht ww Te Lelatry-a- W-3L1X sents. Yo. . TL ego Lac S: [T+ 2 > Joo —. fit, 2ahinbiJ & BM allie Hel S: Tplibes . ca Boo /a litle. sw purbable Mel - aited, Skalent ~~ 9 Nid— werverd). Tee¥ Uwe miglicore AU. Egliad lacs - 17 Copan WilS x W=54 @, lec fae’ 4 Leccernee ot + Lteemern2 Lars Lean’. a wo “cpepve sel. Hum ca SU Lag - Ack fran Lac- ceegerercof flo) (B,) (tat) (To¥A, HulranS mn EMO ba + 7 perf ole Sores. I: 2: ~~ + aad af a. — + — + ": 7 te “ne af roi rook TeTanwe + ond — mba ENA. Feat Resan Tforurd Clacernnae : Pref the bac - ‘. Mal — 5 /32 arcbec — Holt 2 [1 ae bag- T/o) B 7 TA, Mr - too + Me 2 _ + ~ 4+- N- 3 _ + _ a Nr ¥ _ 4+~ ew 2 ft" y fe — + mo ~ OO — te ES FYB. 7 _ L _ 7 Teel urere Chiu Nel wy eeg anche : ? (Neth an ALE Mal — . hoe + Y6 Yr 57 5O oO LB, 2. fay 3, . w- 378 oy, ©, ~ J: ! G. (A, Z Py i 6 4. @, ro, = BY N 4 n. By 3. =P, | iy ny 9b4 5, Op. (6. a P~y 17 he " B, uc 597 u \ SX Sorc, Wo TH, DB HB QS -~ ~ rhe lw 25/28 6/ TH, Pucterte . a, W -~306. If 2. Mack, ! 198. Stubtont a Nf , lace EMB. AY, a> [18 bee , rally allie’. Mo pape Haat jar ¥ - . : _ Mel ~. oo/ yf. 0-306 x SPM lay Lae & My ve. fap ts acuh vy 206 [L. Puck te Nl te etek, specefert, | HE seunrte he Md- on Tekst | Shaki nd ory Lee. Teet pumefed lect ov Mal. l/ - 306 lY2a TFalk+t b-1- Far x f[-b ~fotltt lac — : L bag — 7 gates see 4, dt sume. bee, q 37.57 / 94 feof hact, bec 1 Stel. beet: BI Melee 29M LY - oo feat J. hey shales Lo Mab- Lace 2. pal- bree >. Melt bees Ui Mal> Laer LiNltt 27 fel -. lyr -ab. Lae - 2. Mahe baer 3. Malr- eae - tf, Ataf- pxe- {4} ~ 530 6 AD th Aor: bde Cee prt, Mu, ” bady —~ eg Lac! ( Lace tet) ap (U- pak bee NS . 1Y3 te ee Vhawhs 2, 1908. fo Rep. Yr0 /VB willie | Ca >] SSO | gr plelir > 2evo. Camaprerur relma eTeiabed mt, UW ostil. 5 wulalr regrerud a) seers aoa . [. uth 6 en eS Sous , vont - 32 an on typ. I> 22). w- 2324 Wwe- 335 hil ser atc Mah. 3,1478. _— 110. Dremereye hablsts onan ageneed YIOS + YI0F. p fan brit cables, wool nm LO. €Pnuak Lr BS6 FE = fos. ie 387 F 6 yoy to" 38% FS y1049 yo? 3 BS FH v109 2x10" QR ull hes ded x4 2 ~ Whpeces ry) al lvg EMSs: W108 Y/o Maprare — t+ 1%o Sobrere 1/s - - liucore 1%. * a K-' Melee ¥/0 T 34, -“ _ BIN. helen ee age WosSs Sh, w -708 ~ w- 14s + iW) - tam | be W)-327 _ wW-b28 - , 4S Neth o-f alt portant amet nh del “i> oo Mellrpre | = olé+! _ H a-d - anerlt x» ttt tte WHE W119 ws Ae Cay mrt? te +r $e Compact SYS, pee LES oh, (et 2) ¥ idcote pecehedele Mair of. We deli rtatle Thagle K-(h W-SS wd w]o8 YS 327 7 SOG. 35/8. | Sec 197 foe "WW" stsdiseren Iter 4-1? dail oe Vicheunl ule: "3. bo. AS. AT NEV I. Y/0 4 + + ' 2. Y¥/0 + + . 3. YM. + + em Y K~IL - + + U fra din p'accte! Wo) SNL = = Ulea). 6 K-fe = + lags yl? Heth ge Anca wt cb ctcte,. wel rye gt, m4 byfllrg ifn MO yt fee Lhyelag’ tei. + TLA, va, Woo 7 FE Aen ke lin, frat . 14S Therk, 6.1998. EY B. bac Ne yok. Nak = Anal Aly on Me 1 314 ~ = = Pe Weorth, hubid 370° $321 te Uh UD et + % ~ (108) 4 322 _ ~ - + - (108) Hemp -! f 323 ~ t r * + 7 ” b 22y. ~ + ¢ + 4 * 7 32> + + + + + 32 - -* ~ t + - blu- Heyh-! Y 108 4 BD ~ + + + + 4 es )0 3B2 - +7 + + t * "3933 ~ + + t + + iv >>7 ” + + + + r (> 335. ~ + + r r Ma: 324 LS 50 Ww Tre s see 1. Te tp- | ttt +++ W- 60 “st Ww -102. tet +tr y \ot ads. 1g aad —~ ( w- ly +++ +44 vy V4h W306 — ~ — shy w- 327 oS Khe 7 ~ ~ w- 328 - - - Wer VF - - rect oy ag §. W-243, baw te © 99%4, Teatmn Glu Nob. bo: sd blr MSR. Mal 7 bi WA14S Hegel ta Hethy weber. Tootmbac. b+. fictne bua, Mel. W125” Me-anb. tH © Alt feat mac. 10 +. tJ -120 Me -sp. ba CH 42 WHS Hest + te * He ee allt. Ps. Pq. w-Uy Tae. Hy «| EI%hr. Teatom saatfrae whh+ . Teter + foc. He +, +. WHbO TA t+ 60% weber. Tertrns curltere, 5). RACK ony trelighpas : oo a 5,0. Der lelebee. Fo we-tiy tials tantly dealings fon t's, j aaudl Mrbirtin ple (+) ard bre (-). Prpdlee fron 327, (08m prelaber hdr 327: 4+,2- € Lebaha. 149-I-6 lof. Aeo- 144= 7-10 (11,7 5.0) Whim Beat nig detondbont Cer unteer bolt, prot. )3 0 Maes byé, (95- | Prcpace wasted eeagberee tone ¥ pele Hk cach oh lac LYS Nb. i (ed Ae 2, wIr8 ~ 3. W134 — J VE7TK W4A3 — 5. Y8TX LofoS” ~ O.VET XAST — 7. Wid * wIi3F ~ S.lugax wld - 4.N 87. - Me aiken f reonbrulin, Meychieeklice nua be fred, Mag bore —2. Maul, 0-12, )4¢2- Yiox YS... + a A) CB) pala. ) bee § 18 2. / 26 Shrake ov aC Wn: B). bacSK, per . All. Recount AM + _ eo Lp, | MH YoU 323 | c a. tee) a S| 1 habe rue ol 7, * Loe- = 7.5% A wth &.6% - je Tove 517%. fec-2 Cegan Peeks | )52 Wade & (649. Coots om tae (0) Waeser 237 ot Ue Lh, 3 paki cat "(, Jack seme.) 1 ws, en bo T X,. - x bcshid 2. Blac, 3 3, Sbirlaglir beboflarn, 1 Memmnbmaben poy Thats 1918 Tv (B,) Yi0 + VP7. Measiusd) kage Lelie arerptrerins + tok yo] pte) anh 0+ 7007 Py + 3S meg, Blethe Munk Wrlirn, . A- Pio (on 6, ) 33 /% d,s | m~ & B, 34 / 4, 119 Lema) G13 maT. We prone Hato Pas ghee Mew colonia mag agperas te) /2 raped ly ett, See II: Map Lac-1 on EMS plates March 8, 1948. 1. Y-87 X Y-10 2. Y-53 X Y-40 3. W-183 X Y-46 Id On ENS (-2,) poker . -R -S 4p . - = *) Radi fo lin 3 > $0". iy cohip 364d” Com. « Rput AN. : +4+-~—4$ | Me SZ. 1H 27 36 1f 1B da EYS(B, )lee palo 7] 4, . - 16 a. Aut cerita . 16 3 ; 7 1S. 30 lS” a aupeah > 3° > 1S. 16 3 2 j _ UP h (Ad > seckers - 19 q4 63 257. 2270 Jol | 338° IC. Fos, Tlb,) peter . See page following for raw data. 4otals of all experiments this page are: -R -3 ZR S. 445 131 6 207 (.294) .013 0465 Cf published results: ws +S, fs 4,Q1 2274 lo. Seok muinnetly asa bact. R —§ tf ae © : 13 Ss 4s Lace 1y 3 - | 3 va: [ aed ass 4 sq | +? “ Y / yn 13 1B. Ae bec O oO a 2) Oo o ls S ° ° 12 q © O /¢ 6 0 Oo 13 7 ! Oo 13 S / 0 1S) Ss Mo let - u4 / O O 34 ! © © tat 3 4G OT aye [sry | 270 09S Hes 12367 ( Ic.(plfos 0, shot). 16 80 = ah lo 13 7 26 0 34 14 19. L 1324 O 430 YO ~ 131 6 267 10} Ys v f .29y 013 Hb QT v | S Tes | (S56. ALD 6 crcbrid colon wre sect, Thecame wrees pif ad abd ct TI. ACO 12 cellar wre uf. BN hac v, Th OQ ~ \V/ - Y R ~ = = a oh a. + + Ss - No . X, +R x, Re th cabewkeding the hunarel X ea) om bee Vy Ye Th ia theuld Any conrecdeud) aa wa 2. ey yt Se Se ra na Teed oy | PPrssspaesen A bac - > barr. EX (. B, 1 + Py 3 B, By 48 B >. 8B, Meo, feet Y/0 oan fee nae + ho Te ~ 2 TLO, +h 3 Tre r Y. TL: et & TETRA, +t 6. ee oA le) - (Ok mE “10 sluccose, MVNA | fe ELT8 Pra. We papilla com na flee poke | per bactere. brretoyese 69. Madr 31, (478. Jet shame on dace, opi-Aubre, restecher + gabe ecsrh ba VK Rabie tne Lbogtt. He bac Meolec Gps dae. Nahutbetar C Henge. t RVD +? -%* 4+ 7 + De YO + - to + op b fee, YSS -- 7 _ _ + ee f bees WHS - = - = - re 5 acy 108 -- =P = t+ OW oe 6 lene, LO -1 - - — - - 1 dacs WHS p= - = + g la, W-S p) - + - _ 4 lee, W-Id3 - fF - = - — we oS, weg —-P oR - = T w-252 + + + - + Ir SR w-328 + - + - + 13 pp2dh- W-254 + — + -/ ~ : tet dey i OS beetle, Me erectoorr afb — Sela ot popable 4-12 Wis efera, 04, Oxfous, Teattectreces mutator. + °3-.' Yate + eW- 34). SEP bet Oe rrr. a/b f rtrrbale 58-161" wile YS oh a i ’ : Tilo) + Videclere. 6575 EAP. I}]—- OF. : IES Set hile He te 4 Dba hee /~ a le hy tb le | So ; tiie Bd o i Pugar ISO 4. ee py Sees K ~ ? Co LA a WA tre / 04 Le fore scat: ues Lath un. Sonl€ syle Subimint under Moet : Py — Pio. et - | enc Aik Uane- Aas TOnb wah, . res a 01 Andel 5 bnew Lo Ae 1 Sern nk ag 3.57 As 4 sherntect pose, i ela bed Ifreo walirig we ce, WH Oy. (G0 alme elke, cpp pu <) ; Je (ork + love, tow rn Melr a lo * Latour here cI Ame” # 1Orng Fae So jo" | bechere (Oma, 1 OB br. or ee yr 54% ~ 114 = on 282 Nhidael 163 62 thee. 5. a | m 20 ” (amba) € Iheay 169 W125, W145 april g, 1948. In neolactose tests it was noted that W-125 and W-145 were positive or slow positive on lactose. when streaked out again as controls on outcrosses, this was noted again, and suggests the need for reexamination. Streak out on lactose EaB and compare: W145 stock slant € 1% bac— tolonced , ~ Caleade grail macall, H-145,lyophil tube ACL Syn = Mak bee. Recor te 5 ed, W-125. Varnacroun fosih, aeell sod telnriis Hab wag ht dee coraralrrf shew . Waters tut 14 ealprite hear at ae noig, GC be lat Phaay unnusrrd. Thies acess 6H or telny (70 LauctSgitol selection Galactosan u apr. 9, 1948. Inoculate 58-161 or Y10 heavily into T(m) TLB]BM with 0.1% sugar. 1. Lactdajtol Y10 + +4 re lett Tuk amt 2. " Y10 + +h 3. " 58-}61 2 H+ tenet Ae" be A fea. 4 " 56-161 zt pay 100 ml. Ars _ 5 Galactosan 58-161 _ - ~ / beer oe 6 Galactose 58-161 +e. ~ +t— AE. Arle aI land d. [iver Anke . (3) [ wm lacldof Ach, war +. , ee ey AVA. 5.0.) woke by ek W349 gare SPE, ly d¥4 wo pcs bel tht eu lately weak ; 56 elwe ict Lacrito antes Tie, FH - 1%. {from Uso Lebrda lt) K-1L — : YJo ~ YS 9 - WHS = W-108 — welds = W-lvs W-124& - W133 —- K-L Nek — +54ec 56 :6¢ Melt — se 0.170 pornos of Like aubwodks. L j A ae emerge WILT, Wys. [Aeron lact ap rhechensy Wis « Fe, TP~/6/, + a 16 (6 be - Vive elms an, EMS Wwl>d x SP -16] + - 33 S53 {97 IL w4S x ¥/o >/0ee) — y 5A 1GE- ee N wio8 3 ed. + + - . ged Holl hot an 25 3, a 1 f bette votuctin; . ger Ul DY tg ae UW rl. Wale. 40 5 tw ud Drrovn bL-hretrnor EMC. ee fen one 1 pee hoff tt bAcos 3° a . 78 ) 270 Ha Se. Leo ad~x eee EMB, SO pat . ta [Be acontatlte calecccn pupal JI 10- CH: SO TOD tp lerscs - 10 "ectorcBs ° WwW X Meat we tal eae TI 35! | ~ + 4 BS 2 ~ ‘ 4 353 3 _ asy. 4 ~ AT - b a. p _ - + + 9 — H + + L 3 bo /o — solo} a _ Jr 4 ~ ~ — y + ~ a + = L t + + +- Kk t= 17 + — If + -- 7 + - 370 Yo + — vt + - ~ + r 22 + 23 +- — | + + Vy a — 2 a a — L % + - < 4 + = V nn a a 24 — — _ _ i _ _ R 560 40 — — — __ - _ _ Q / 3 y 5 ¢ 7 € FHO+ I%o glucose. +. flack Ar/b, 1 VAF. pre powth - “SRE Meheak lords fmrsbeunt. BIS lage colons Ney i gorb eolelirg Ler AGE Apher kt hie fenebherny (oF S have ,prrsale telrrees | 41%, Me ale | rlcrte EM, fekita; hogy fp. 25 1e- Ley 1h tro — _ — p87 tem — = + }72 4 aa _ + * + 1849 “wa, OT _ —/ + We tb ++ + 5 +, 237 pap ~ — 7 as COC - - + + nS 7 + tr the — 73 + > + +. 319 — ” - _ ae . 32) tr pp ++ ++ += “708 = pep. mw pe mp He += (ee Mab bee Fok Ane Meal HOHE NHHM YY Broth 7 Creates Quepasa? fran fall DB cull n 1 fn plan 3.0. 32. oT fe ~ 64. 187, U8, 17> ia CN I7 Pa. 5.0. 291 m Gite Fo%r. [safe Wn ~ fo tat eo Lec, WYP a , 0 ALR. 7 — UND a Cte a Nes Cpa Cod ; LY5 or btore . at a fet * tolnuin prlnernale ert, feat i kee en Ble. | / 708 par — _ +b ++ _ tJ/0F WHShe- .., Spy ll rs - + = ISY, 1-3. Mdlackt — t + + — th Ww3él » 2VYGkac- = = + ++ —_ 243tae- 2 — + + + —h- wiryva fshicale prsfud wok on lac YY 20 copasalhe To wW)08 omaha be Lees =. VV obae tary Bea Seraees fre. Cll rv¥3lee - Wr an errr anrrel VVF bac te = WIZE] Pree attie Ll tere Arbar. W & y beled : Cokaecee anal 4d pas o vw foe, 9S + ow Hey Mauer FU. Sottf Ml- Faeteae Aten. Rermrer ; a. Weer glu - ard wth + on linkd) wrnisaf: . hemtertoe’s Gory 1778. May 5 1998. Shubert 1774 , W-r¥s [ek on7 Wal E17 p>, Prete MY elt cgtmieds io tae and fhe. ap 377 a) KC Pace Lactr Bly - 4) MAb bt colrrces beet ty ~ | Mok ~— Cooney bee Abe, a4 partes. 3.0. fume aandl b a rr2lbae © punts . W397 4 1W039F Faumuble, fats: ENA. 17@. K-12 +44 » HP de w-108 + ~ yr} 2¥3 + 3 +76 266 ~ 4 83 76! =_ 6 Ve6 167 =Te lt » 8? 2649 - 1 BI 270 + 6 3/6 277 _ 4 >IT - 280 + eo [Pe VEY ~ ” a Les - 2927 - 247 VOA- 299 + 30] _ 307 + goer 7 au ~ 249 + 2357 - 18 = b19 - 322 + 32] + {+o -~ nT aS,1 +++ RSF +e YS3 the Vio +H ~~ * 31. 4 30r we prc fl ath, waits | Sou ? OQ ay gteccore. fhe — Wwutelons ton, ) 8° [pl LO de, HYP SE 716, 8: | dS othe K > 10 Aenet cartrseds = CO (5) rey Rebaf. 1S yy erlbomcen puberd Wore nuabhanty . Vo undone from vu 6 than peice . 0. Apiredion Aetucrar'%h, frrmale 1% totes nee rnolir bey eat 1b pater rr ? 5 bre tobe, — an a } fo, Va, |, 1418. mance. — (dicerse EHto+ ane + (-) ehenees tho f 0 Ce) Come SY, Wane $tyeban, 01% + (0) Fane - ~ frrenptute Miea2 6 ee (a) (6) { EM Sf Sfen. ey fer eine /, yi +e 0 apete o 2. } 3 I- . fo | ~ 3. Ir é. 1- pone oO 7. Yt _ 8. - + 9. a - {d, ~ S- >? wh i (up?) 12 ~ 13 & T a cy ~ ‘So4t - 6 R- - Deaueebls peod diwle tfficrin, flied Maegh aga ” Spee of 1,4,6,7,3,9 Dv» + (41) an Ghecerse EMA. PratesToragsorteable Tet aTicler on pt en “ge, i . oor ” Terma fn 6 Ftd gato 38S 1G] — f a ay. 4 FL [ed W-Id om _ — colores . ~ | ENO ~ Wo Masliecsnphorplite Sto. Lasgn Ilo Peake arid) uutiadin d how MS Tre 3. Neplideyesd) teri, CHE) Oper . Moduake . Mobriake colored . 4. HO~ Sucernala - Mrnghinaticdaphinnch ' Shouws a fparre reeling 0 / se Aertel ‘fe trons: i. Ee els ~ Ss. g 60. 8. He- wot, Feit os ¢ hola dar clennged on prrgracdor CP, dusteccirl Ce Mp homer ey ome yorbated 17 : tre Pehle vo elinns yeas q. ° : fo HHI) : tL) - MO gen cnn arate proluanr, ; ISS Apel 30, 168. Dure Wel YS Wh, ude T lw) TLE, BY + ~/% 244” Th. /. bieonale - ttt 2. SrA etar2 trt t+ 3. bactrre + ++ 4. Haber . + 1 2 » 2.0. P3 An, HL. . Me -97Z,0 Ye J. Mo Pebeerioghespbede > 0.r% “teas et 2. pecker werd) mt, MOH | + + paws aural 1h. vb. T E MB. 58-16 / - + Yio — + p3. S.0. | Sand om hares tones EM agar - | Mh wxtd prartuctn colonite neue auberbeteal 3. Vamures + cols. heh & re EMP Phecemse rnveclling rm ify fw 1418 Wo Idem ete. (Perera bsecs.) am Ene. AA rss hy edt a 20 heart en Yr piles } ca Sop terete = /0, 0%. 3 Blicger — | Stuele aero T/. Ate y * Ak, , 7aal tae. Led Arne |. W- 382 tet tee + OP bee 2. W- 28> - x x — e+ 3. w- 36q - H ~ - te ~d82. Why pepillar rbymn rather ? T tala aipenctte de te keried “Cacore- epee meudeah fon ceaaren wrth Aral peobierts acid shorty tom lifted titan kugpeatine >-3bowo! (Cancpaate 340). as Sheehy ret 384ml 390 a A A SG : 372 ate (FS /.1s4 6 - 2. D. d. -TOB/S & Fe ate oF: IS ¥ frmnttnn dest, . duel nA be tenn ta Rhaunuert Biter Sotrtf 08S — + ~ —~ —- ~ - 9S b+ rads wd —_ ++ 7 ul, a 6s )- - + = — - ~ nee pee 2490 - + = B- - @ oi 3a5/ —- He te Me ee ya BEf - a aR RO regis fo ane D.6 wee hack ++ - t+ tta- ee gt FE hae a bt VA of PA aon (mr EMP "lo :). ctere [laser clef Macmenre He lec fret Drelectet bbe OO w ee: ee th _ wT a _ —_— oe ” NM 7 pol - — + + & th § Be - t¢ “ wk ™ {Leet Maced ae X Ar eS — - 4th 4+ o¢4 —~ = ah mb, —~ FP te te Ab p- ~ ~~ te OT, Ab P’ ++ y-P - 2M oa ne ~ 44 < -_ - ~~ oe tia Lac. Crosses 3 May 4, 1948. Cross the following on EMS-Lac-B, . 1. W-108 x W-249 (A conc.. susp) TWL—B, -Lac, x B-M-Lac, 2. W-108 x Y-40 x B-¥- V1 3, W-249 x Y-46 x T-L-B,-V,* PT. oO ) oO ! O } 0 oO o oO 0 } © I: A. Oo Ss A. ° 3 A oO of A o a ° oO 0 3 © / A oO 2 oO } O ! g oO oO >a © oO A 6 3 A o A © wu err errata °O 38 / () . ' , Ft £4 pLAG aS CNC AR Tanne 7 Co C7. , i J YB OA AnH A tA yy AA Lg | ait dhe ge 27 at, jd 187 /87 ~ 2 — 3 i? 25° 6 ay a2 5 2 e 30 4 sO oO 32 7 aS 4 | 281 = 67% Leese. T-L-1,-bacy- BeMs ( x rota teege it been, 4 fala clnely Lneld te BAC ote we Phage f0k5 (or giccore plates). lace: ¢R 9% |? All Bh + lec-: (498% 33°Lb) Aee — 10) oR net 12 63 Ale | = Po% 25 [ 124. ap pie "ith Lest bacbigrend | O ] O O 2 ° o ° ] © { fo BO 3 58-16): Ca nubation Aun. la¢ 12, 1998. | [ov plalr DluEME x 19> |edate = 28, 00 Pe Relek wtih gece > (7-3) 4 O mruecod f oO t . ar) + 7 Oo + +f) 8 o - 5) 4 ° ~ lo @ t no 6 @ 7 1r @ + I> @ r 6) WoO Hors ? (7) WO “ee et 1b 60 v 17 © r W2,YS aoa? ace Ts Sard pestraleby nunckante . Y, 2 eal acheaeaen alent abs white Lo- f h ae — - + Hour + tr 23 t £ + . 4 588 = th ee 389 ys A eT} i a re t+ ++t " 390 Fi, et ++ et Hert 7 frat fet ett + _ 7 peerfeall hae + Maw 5, IG IE. | 08 «SP-/g) an gore t P, 2. 249 x 108 on pslecee B, 3, 382% 2Y9 a gfutese (acl a0 - Yo B82 xSP) sees lac hose . / : " Pv: € —A,. Oo Ae 14 177 Je 133 35° 3ee0 32,5 1, 2h 163 [184 56 {63 311 rere /9] /f2@ 1448 a ” tn OL TS Viernes. oe . A pr Meal vise milinae dup he fens Mahescirn fru pore , Ure aera kag jada chic Napa il hap Sor Arable. thin, 4t0r wbrret fre Cadell calor trufleinb geuteerboslers lor a f- NG SCS lor Mg. Lac 3 crosses / 9 wv Lac 3 mapping. May 10, 1948 har Ens (We). (OD. W-10? x Y4o. om ond gle (NF | () w-i¥ix yy¥6 & W-108 x W-2Y4. | 3 : . v a Si Sy, ~< + oO 9 oO Oo oO oO . 84 % u UY 3 22 ° 25 oO L Mt a / o tL 24 ° 3/ o é Y/ Oo L 16 o L 17 Oo s~ Oo ree bac: 197! °. Gk! 310 oO FabbwGo1 0 Poth ae p pall hats -. spl 9 lf 2— a Je of 3 2 3 Oo Y O A / es oO 7 O 7 2 — - BLL scarce (Ju, , QM We is [~ Ga © sstrece Ly le [ert’ha *Aanloy 7 ‘y Ly £618 A x ¥f -/6 29 2 3/ 35 g on 4 - 39 Sf 7 > #87 51a 10s] 607 > 11% bce. 93olaec- Tort hort a, Xt, Tl: R $ Le 2 rT 2 13 L| 52 9 ee = 1, arenora, Lee + Toot ber - mm Man AvnT| IP Xf Terk Lee - tegenento on T, ( Atle ox Bena Eras’ ) R S, iS) Ss Lo ae 6 of G ye I> 7 70 56 wl Sou 30% aru lee The birbibtinn xo Hew: ond lcadewtted fener J —R 58 bt -S 25) EZ 126 +R ws Lv 16 + O42 a /.00 jor [ef So reper Estima x foe Ylure Lake - . - Ms Y¥- 2 Hecebs @ > ,OL2 x is /s8 x25 . 6235 |. et IE b = 0tr x SBS IS KUT = .3%0 583 67 © = ord x 12>T/ IT KSPR eg 6 253 26 a Lac-3, Lac-4 crosses May 17, 1948. 1. 108 x y4O On Lad—\and on Gna EMS! 2. W-67 X Y46 On Lae 3. W-126 X Y40. On Lac I gre Md S10 (plete, TeX glucose E87 veo Go L 32. I bee. a 4 leet: 6*: 15 ate Ar + 20 oO bag — R S Ne le - f Oo +g b 7 2 4 by tears feats tort rotor ° 2% , pee wal 3 i It }e “late? t 4 82 33 jus 3 oO 3 oO /0 2° oS oO é O tf £ 3 ° 3 ° 2 Oo , 302~C«~S™S*«*Y'B S/% —" The Mlibute, od Tae U® ye 28.2% £S J ~” -§ + R +38 687 215 OU 007 177 — W-67T x Y-46 | 5 > PH-leey~ xX Tel-fy- uF. ! o R S | 0 2 oO 24 62 Od at. / 0 / d / O 3 oO a 0 an) Y Oo Yo / O rn) 4 o / 0 32 0. |! R S ~R + — Lac- i 4 +R $4 ; 7 _ tN 6 -- § or | . ; , (7 22 jo | 3X +8 b } lect ath 8 P 83 Mucs ) °o , . « Lb & Wy 8 sp ites ! 5 (3 17 6 [83 |b 33 rg eT “8 fim 31% (; [Tag be xcesent) Ler Uiise paleo / — te Corecess MACIR el gr ralle, Ha., f pera ducctein. alee. Summary: Lac-3 mapping crosses W-108 X Y-40. 200-3 p/187: 174 : 254~ on lactose. ie 6.7% Lac 4# Among #, 6V,T +: 21,5. Fox ogame - 99 + 25 Lee ~ , /191: 564 : 463- i.e. 13% Lac 47 ye ~ 22.20 Pp? < oo) /198: 1054 : 512- 17% Lac-/- among 4, 526788 13% Ss. Among ~ 56R:24S (708 3) among Lae-., —> if /. x4 72,83 p = OF peg" 194. bbeme- 130 >: 7 + 5.)% bacs+ =~ 30 + a. BEC. to bac - > ley. a hae te Rhett on tre teat Rf. = 3 of Pilye, /. ee 20. fs ii o tH 0Q Saliem Puy adalin + Ht + see + xe _— es Eco b.wt Fee heubeckn delmacbh & typoler 4+ + + t + + + _ + — f& CH, -CHon- CHO + F © ”™ HC -CHy, — _ Alo, —C HOR - + a ~ hiale-c00e L~ Matrnere d- Ribose L- K nove D- Lyxose p- Kylose L- Rhamnose xy lorce ar. + d- nettle acabrneside _~ >be rulndle — + 0 mitted) Hamel — a- etalte . K-22 it ttettree + + — | ct + + + + + 4 + + + + + + + + yoo Maire grate sorbulef dudlc.led Inosrtek Milne Co thine. (ofa: “3 re lt + T + | fete KP Gt ty Gt fuctalz Sebel + + + + + ° + F + + + + _ — - + + + + $- + + - _ a + + + + + + + 4+ + - + heeitt + Oct. few WV + + te +7 to +r + 7 + 7 in He Tt 4p wbuconle fd Hucoasth - fh qolactoale (lacks adap )