OFFICE MEMORANDUM e¢ STANFORD UNIVERSITY © OFFICE MEMORANDUM e STANFORD UNIVERSITY e OFFICE MEMORANDUM

NEC 3 4973

Date: November 30, 1973

To Joshua Lederberg
FROM: I. Rabinowitz, Ph.D.

D.I. Wilkinson, Ph.D.
SuBJECT:

RE: NIH GC/HRMS Proposal

Research carried out in this department has strongly implicated a role

for the prostaglandins in the etiology of psoriasis (E. M. Farber, K. Aso,

32nd Annual Meeting, American Academy Dermatology, Chicago, I1]., Dec. 1973;

E. M. Farber et al, J. Invest. Derm.,in preparation; E. M. Farber et al,

Nature New Biology, in preparation). The prostaglandins are a class of

Cog fatty acids, having molecular weights near 350 and basal tissue con-
centrations in the nanogram and picogram per gram range. The prostaglandins are
presently detected by radioimmunoassay, bioassay and mass spectrometric
techniques, among others. There is considerable controversy concerning the
method of choice for measurement of absolute amounts of prostaglandin in various
tissues. In particular, it has been suggested that mass spectrometric techniques
yield more accurate quantitative assays than radioimmunoassay techniques (Adv.
Biosciences, 9, 71-123, 1973, Ed. G. Raspé, S. Bernhard, Pergamon Press, N.Y.).
Radioimmunoassay techniques are currently in use in our laboratories, and

the addition of mass spectrometry capability would greatly increase the
definitiveness of our studies, as well as make available to us a powerful

tool for the study of prostaglandin precursors and metabolites. Work to date
has been supported in part by NIH Grant No. AM 15107.

Dyed \ AOD on Sm
D. I. Wilkinson, Ph.D.
Department of Dermatology

 
     
 

 

  

I. Rabinowitz, Ph.D.
Department of Dermatolo

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