OFFICE MEMORANDUM ¢ STANFORD UNIVERSITY ©@ OFFICE MEMORANDUM © STANFORD UNIVERSITY @¢ OFFICE MEMORANDUM

To

From

SuBJeCct:

As

Nov 24 1979

Date: November 28, 1973

Joshua Lederberg, Ph.D.
$331

William R. Fair, M.D.
$287

Use of facilities for high resolution mass spectral analysis with gas
chromatography.

your memo of November 1973 requested, we have answered the questions concerning

our interest in GC/HRMS.

1.

This service would be of definite value to us in two projects currently being
investigated in our laboratories. a) The identification, distribution, and
biological significance of the prostatic antibacterial factor (PAF). Our
preliminary experiments indicate that this is a basic polypeptide, perhaps
attached to a divalent metal such as zinc. b) This service would also be of
value in the determination of the urinary polyamine levels in patients with
various genitourinary tract malignancies. Our initial experiments along this
line indicate that there is significant elevation of polyamines in patients with
prostatic carcinoma. The use of GC/HRMS would enable a more precise quantita-
tion of these differences and enable us to expand our research into other areas
concerning the biochemical significance of the polyamines.

I would estimate that on the PAF project we would use approximately 2-4 samples
per month and perhaps 10-12 samples per month on the polyamine projects. Both
of these projects would require the use of GC/HRMS.

A portion of our research on the PAF is currently supported by a grant from the
NIH. The amount of this grant is $36,698, and this grant will terminate on
December 31, 1974.

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