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INSTITUT DE CHIMIE BIOLOGIQUE

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a FACULTE DE MEDECINE

DE STRASBOURG

STRASBOURG, le.

INSTITUT DE CHIMIE BIOLOGIQUE
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Under the «ction of the DRNA o f C1S, C2R (deprived of the poly-
Saccharide and capable of fermenting sucrase) is transformable to C1s
(containing polysaccharide and incapableof fermenting sucrose.

We have tried, wy cpllaborators and myself, more than a year agp,

what would be the effect of NA of CLR on living C2R. We had obtained a

a strain of type R capable of fermenting sucrose only slowly (while

C2R does so actively and Cl not at all.) but we hw e not been able to
trensform this R strain either with WA from either Cls or Cés. Unfor-
tunately, since then,albeit with different preparations of the NA of
C1R we have npt been able to repeat this transformation pf CeR
vigorously attacking sucrose to an R which attackso it only slowly.

On the other hand, when C2S passes spontaneously to C2R, one obtains

a ‘spectrum! of variantes distinguishable by the appearnace of the
colonies, Only one of these C2R variants, up to now, is capable of
passage to Cls by@the action of NA of Cls. But in studying, subsequently,
the other (non tranformable) variamts we have found them to be
tnequally active on sucrose, m6 we havefound (b; spontdeaas occurrence)
one which attacks suuvrose only veru slowly. That pulls us into a
quandary as to the significance of the loss of the power to ferment
Sucrose under the influence of NA of C1S or C1R ! Nothing has been
publishea on this point, while whiting for further experiments. My
departure from the Institut Pasteur and arra&val at strasbourg, in sésponsc
to the ‘appeal of obligation) made to me b. the faculty of Medicine

of the Alsace (where I have worked before) has momentarily disturbed

my research to 4 considerabel extent. I am encountering unusual aiffi-
culties at the University. However,I mave already re-undertaken my exps.
tp a certain extent and have higher hopes for resuming my work under
notmal conditions,,I am informing you of my observations and comclusions
by letter, while hoping to meet you this summer at the Congress at
Copenhagen,

In conclusion , the sole point, at this time, which appears to me
to be settled beyond doubt is the rossibility of changing the antigenic
type of the coli bacillnts by anh inductive principle exactly as in the
pneumococci.

I will add that all my attempts (they were numemwus) to carry
Clr to Ces by NA from C2s (and also Cer) hase failed. Similarly, other
DRNA anc RNA frother bacteriaand animal thssues have not been active
on C2R or Clr.

I agree that your arguments in favor of the intervention of some form
of s xual phenomenon in bacteria, to explain your recent observations,
harry enormous weight, and after having reflected on the. matter at
length, I am all but convinved that you are right. What you heve told
me in your letter of the 13th reinforces that opinion, Do you not think

that one coulda find the occurrence of aome heterokaryosis such as is
found in the asexual molds? I mean, in particular,the publication of
Pontecorvo, Nature 154:514 1944. Parallel with the chemical studies
follwoed with my collaboratory Vendrely during the last few years, :nd
which contuhnues. here, on the N* of various bacterial species, I have
undertaken with another coll& oratpr,Tulasne, the magnificent work of
Robinow on basterial nuclei. Tulasne confirms Hobinow in eve: y detail.

But, in examining the preparations of tusesne; 23 in a0 preps.
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Of course, Since kka your new forms are stable, it would be necessaryv
to visulaize not only a change of nuclei betweem bacteria,rut the reaction
(fusion, etc..) of nuclei of different origin in the same bacterium.

I am very much impressed with the interest which Prof. Vatum
attaches to my work, and thank him foe the friendly regards which you
have transmitted on his behalf. Would you, in return,espress to hin
the great admiration which I have for his admirable researches.

kwaxkingxyouk Hoping to hear from you Sovily~ and 00 uieet you
this sumer in Europe, etc..
AB