STATE OF CONNECTICUT
STATE DEPARTMENT OF HEALTH
SOUTHBURY TRAINING SCHOOL

Sourupury . CoNNECTICUT

06488

 

August 12, 1966
a
Soa L

5?
Dr. Joshua Lederberg A ws ° *

Department of Genetics

Stanford University, School of Medicine
300 Pasteur Drive

Palo Alto, California 94304

Dear Dr. Lederberg:

Your letter of August 5 inquiring about our work on sex chromatin
determination on human amniotic fluid cells has been forwarded to
me from Yale. I am primarily situated here in Southbury and have
aclinical, part time association at Yale.

Enclosed is a reprint of an article by Dr. Steele and myself published
recently in The Lancet. You will note that the primary purpose of

our study was to investigate the possibility of growing cells from
amniotic fluid, whereas the sex chromatin determination aspect was
only related and mostly served to confirm the work of others, The
note in Medical World News appears to have emphasized the latter
aspect of our study. I am sorry to report that our further efforts
to improve the culture technique so that this might be a practical
procedure, i.e. determining the individuals karyotype prenatally

have been unsuccessful.

Thank you for your interest in this work.

  

W. Roy Breg, M.D.
Assistant Medical Director

WRB/fd

339