October 6, 1955

Dr. Norton Zinder
Rockefeller Institute
New York 21, N.Y.

Dear Norton:

Could you refresh my memory on a point that has come tip in connection
with some further serotypic analysis that one of my students (Tetsuo Tino)
is carrying on? He is anxious to get a lysate from a TM2(PLT22) culture,
which, as you know, does not induce very well. But your thesis comments that
FA could be obtained directly from TM2 by the use of crystal violet, anti-
biotics, etc.

Can you dig out the details of this? The presumption is that Ta2 is al-
ready lysogenic for a phage for which no good indicator was available; have
you any other information that wuld bear this out, or that might be of prac~
tical utility in producing FA from TM2 by chemicals: I do not have the data
on the concentrations of the chemicals that were used.

With best regards,

Tours sincerely,

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