WASHINGTON @EH} UNIVERSITY

 

SCHOOL OF MEDICINE
SAINT LOUIS

DEPARTMENT OF BACTERIOLOGY
EUCLID AVENUE AND KINGSHIGHWAY June 8 , 1946

Mr. J. Lederberg

Yale University

Qsborn Botanical Laboratory
New Haven, Connecticut

Dear Josh: ee
1 received your letter of the sixth. For your purposes

I don't think that Octosporus would do, since it is extremely

difficult to keep this form in the haploid state. Pombe would

probably be preferable, I will send you soon a copy of my stock

culture which sporulates very easily so you will not have any diffi-

culty isolating the haploid forms.

I am puzzled by one statement you made, namely that you
would be satisfied by a form that could be obtained in reasonably
pure haploid culture, but which can be made to sporulate, if need be.
As you probably know, haploidy and sporulation are mutually exclusive
so that once you have a culture ina haploid state, no sporulation
could possibly occur. One other point I would like to raise; do you
have any purpose in confining yourself to the schizo-saccharomycs or
wouldn't the saccharomycs @o just as well. It is relatively easy to
isolate haploid forms from sereviiae.

We shail welcome your brother with suitable pomp and circum-

stance. I expect many fundamental findings from your efforts, so
keep me informed. ’

Sincerely yours,

Sok

S. Spiegelman

8S:mm

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