[written in Lederberg's handwriting, Yanofsky]
May 29 1958
Dear Josh,
I'm enclosing 9 stocks which have not been messed-up yet and two which are in a different category, as you will see!
T-3 [unknown symbol] ansh (h k-12 by U.V.)
td4 I [unknown symbol] T                "
T-4 AA [unknown symbol] I            "
Hist minus cys4 reisolate (k-12) from Treffers [?] -- a fairly good stock if grown in the presence of cysteine supplement (30 gamma/ml) -- all In rich media and slants
"Hfr" H minus T-3 -- transduced "B" tryp region (with requires) into H minus cysq and isolated an H plus with requires -- replicated for an Hfr (1) and got one -- replaced tryp region by transduction from H minus cys4 -- picked a H minus cys minus -- replaced cys with T-3 by transduction
undoubtably lysogenic for Plke --
This stock and other isolated from Hfr(1) gives 5.5 percent recomb. when crossed to strain B F minus's and to avalent K-12's. We only get high frequency when we plate 1 hr old mixture on enriched minimal (.2 percent by volume, nutrient broth soln supplement) and if we include a supplement permitten, recovery of the non-hist marker of the donor ([ . . . ] A. with HfrH minus T-3)
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Recomb. colonies tend to be se[ . . . ]ed. Really don't know what grew with this strain, would appreciate your opinion if you can run a cross with it.
Hfr H minus/1tryp number delta -- mostly organic for Plkc from Hfr (1) by exposure to F1. Isolated typical tryp deletion type -- responds to tryparue and is V1R. V1 is of course very closely linked to the tryp region. We have been able to transducer the tryp deletion into a cys attach.
8am order a basis of crosses and transduction.
[diagram]
If any of these prove useful let me know and I'll send you a copy of my stock list -- we have many combinations which you might find useful. --
I'd like a transfer of the gal stock you think is closely linked -- it might be close enough for our transduction mapping.
Hope Elway will give you what you want -- this place will probably continue to be almost unbearable if it remains as is --
Best regards,
Charley