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Aricckian Aon....One: of. the mire. exeiting eowstimld was about centrioles considfred for pyasmids, whigh came up obliquely in discussing verdous experimental designs/ ~~ in re nuclear transplantations. I had not realized how sharply dependent the frog egg was” = _.... 00 & sperm “or center", having thought that pricking would activate cleavage as well as. a the initial fertilisation reaction. But Bob had seen that experiments: involving, ¢.g-5 blood —""—“ge-@ Nea gent did not exclude centrioles; this is of course the dnewer to Shaver's problem of ~~ _ the "seepnd factor" in parthenogenesis; Briggs was sceptical ahout the extent of any.of the activations with ill-defined material, as Shaver did not. score his material very late, and his yields were very lows What excited me was that the attivated- unfertilised egg wpuld es _constitute a simple assay for the biological activity of cantriolar preparatipns, qua plasmid, with all the possibilites for genetic and chemical ahalysis. They already know that Tritarus ~ Svradiated sperm will activate frog eggs, so the specificites are Low/; it is even conceivable _.__...that mouse. centrioles will function in frog cytoplasm if. any of Shaver is to.be rekzevant. here... Anyhow we did comverge on the importamce of this, and Bob may put a fellow"Subtelny" to the ~~ “problem of pulling apart sperm, and perhaps otter cells, so define just what pie activating” — _. Genter ia. .D would almost be tempted. to take a vacatipn in Philadelphia: myeelf.. 3... - “Note from Kalckar and Kurahashi: Gal 1,6 and 7 evidently all lack the second “enayte;- ~ — | galactose.I-phosphate-uridyl. transferase, Which is the same_as the. galastosenic infants. _ Esther took a phone call from Herman yesterday which sounded rather confused; 2 don't under—_ ~~" ~"“etand the constitutivity & the enzyme sequence myself. Anyhow, these mitants fomm one _..-.-. position-effec t. group;we have to send. them Gal. zee see. 4f.these_are in any way ==. diffe rent. —In. the. lab. only. one . new. points . the effect of Ca in. stabilising spheres « of Kel2 was con~.. ~ firmed; it now remains to 1) review viability of these preps.; 2) biochemical properties— p per Boris, and 3y uy ‘to grow g-gn So far, nothing promising c on | non-lambda transduction. ee ee eee - *. | OP MAB 9. ane nnn ee ne ee cee oom Many F35--1956 (Sun) /~ Pontecorvo visited last week; arriving several hours~----------- J... -_ date owing to fog, and only just in time to meet us at the Hoffman House .§ —ss—s—> for a dinner honoring Jim Neel by a local medical student fraternity. But To we had tiskets to the Dublin Players, to which Pontet escorted Amn Crow to --- > oe 00 Armes and the Man, rather. than Neel's lecture on hemoglobins. Neel himseld is in the midst of a changeover to a Genetics Department at U/Mich Medica} $$ ehool, He still construes this rather narrowly as human genetics (my ow hopes a would be for_a department having. the.same_relationshdpto medicine as ours does to Agriculture.). For example, he had projected some tissue cultures studies "en fuman mutant material, but for biochemtcal rather than any hope of genetic oo ee a lysis....We discussed Newton Morton's position at the medical school here; Jim had also talked to Mortenson, and I feab there is a growing misunderstanding on ~~ ——————Netwon's role in Anatany. (I also ran into Phil Cohen whose apprehensions are ~~ ___.___..___ ...-.@ven_stronger, and he is. going to reopen the question with Bowers. Newt' himself = _ desn't seem to be strong enough to stand up to these pressures, and he is especially ~~ walnerable just because his research pregram is in abeyance while he learns laboratory" ee genetics.) It's too bad we don't have a stronger atart_(in add. to, notin place of Newton-- I just thought of Mitchison as representative of some of the other hopeful directions, This did not jyst cane from the bluey Ponte(t told me that——-———- -_-—__Mitch_had_just.done_the experiment—on ion_of cells heterozygous. for Ho which I... _ talked about at the Ascites meeting, and which I suppose G Klein had also planned, +... .--. We -had an intensive. going-over with Ponte, after which I feel rather pumped §.§_-—| dry though this is largely my own impulse. It must still be barely possible to do some mapping from diploid automixis in coli, but the worst problem is = ; anne oor bopgakeing into the cycle of circuler-reasoning on the location. of -markers.-This——-.—- oj. as deen something I had hoped to find the xight student for, but he hasn't = fos materialized, and Alan seems less likely than at first. I don't really have much ————-__—-wopthwhtie data, -but -now-that “Gals -is-out- of-the stocks; and -we-can- seore Lac; ve — Ww. Lac, fairly readily by progeny teSts, the problem should be simpler. The best designs _ at first might be te select for crossing-over of near--linked genes. For example, oe ~~~“between Lacy 7 aud V¢, selecting for ¥g still Lacv,- or between ye "Mal andS (+8/-r, selecting for +r/-r) and seeing the ditsribution of homozygosis — for other markers. ‘But we need “the right marked diploids first, and-these- msy ~ Jus’ an -—.--— Mow be coming through fron LacjHfr x Lac, crosses, the stocks for which are aby product of Newton's wOpk. _.W-...___.__._ Experimentally,.last week was only a couple of days between vistors (MIW) which —_—- concerned mabajy more on "protoplasts" (I like Stahelin's term gymnoplasts better). TO HS expe perinents were rather N6ESSY,— probably” because of” haste, “and perhaps because a ther. (At. least the planting is es- ue. Summer started abruptly with some muggy 80+. sentially done now). Viability has been variable, some prepa showing at least 50%; “"~——~~9thers less than 5. No show #0 far on an L cycle, certain that the protoplasts do make NPGase, but even the control rods_ are not too happy about the hypertoni&# sucrose, and it may take some more fiddling with the medium to perfect that, if at all. No more tra&is yet om DNA =