MAY 124975 cs)
THE UNIVERSITY OF ROCHESTER
SCHOOL OF MEDICINE AND DENTISTRY
AND
STRONG MEMORIAL HOSPITAL
ROCHESTER, NEW YORK 14642
DEPARTMENT OF MICROBIOLOGY 6 May 1975

Dr. Joshua Lederberg

Department of Genetics

Stanford University Medical School
Stanford, California 94305

Dear Josh:

Under separate cover | have sent the strain for genetic mapping
RUB830, which is relatively promiscuous for phage DNA grown on Bacillus
amyloliquefaciens. That manuscript will be forthcoming and | will send it
to you as soon as it is prepared. In addition, | have sent a lyophilized
strain of the B. amyloliquefaciens that we used to isolate Bam. The enclosed
outline of purification procedures will give you an indication of how we
handle it. If you have any problems, do not hesitate to contact me. I would
also greatly appreciate receiving the strain from Bacillus globiggi that you
have worked with so that we can work on its restriction nucleases as well.

Thank you very much for sending me your preprint. I think that
it is a great step in the direction of gene purification. It is interesting
to note that we had done similar experiments as yet unpublished with EcoR]
utilizing the same relative group of markers. Our results are similar to
yours and I will send these to you when | get a chance to pull them together.

During the ASM meetings, we formulated the program for the upcoming
Bacillus meeting. A copy is enclosed and | trust that you will be able to
attend and participate in two ways. First, | believe that it would be highly
appropriate if you could present your recent work with the EcoR1 digests
and the use of other restriction nucleases on gene purification. In addition,
i would like to invite you to summarize the proceedings of the conference and
to give us your insights to the future of genetic manipulation in microbes.
| believe from sitting with you at the Asilomar meetings that you would be
able to really contribute a great deal to such proceedings. The meeting will
be written up and published in the book MICROBIOLOGY. Because there is no
copyright and limitation of republication of these data, many people will
be submitting their manuscripts and thus it will constitute the first compen-
dium of a volume dedicated to studies in Bacillfi. | will look forward hope-
fully to your participation and to meeting with you at that time.

With warmest regards,

Sincerely,

Frank E. Young, M.D., Ph.D.

Enclosures Professor and Chairman
Y/c