PROGRAM

INTERNATIONAL CONFERENCE ON RECOMBINANT DNA MOLECULES

Asilomar Conference Center, Pacific Grove, California

Sponsored by the U.S.

2. 23-70

February 24-27, 1975

National Academy of Sciences with financial support from

the National Cancer Institute of the National Institutes of Health, and the
National Science Foundation.

MONDAY
FEBRUARY 24, 1975

 

 

 

TIME SUBJECT SPEAKER
8:30 Introduction: History, Aim of Meeting, D. Baltimore
Outline of Task (MIT - USA)
8:50 Assessment of present technology, Pp. Berg
joining methods and vectors (Stanford ~ USA)
9:10 Ecology of Plasmids and Enteric Organisms
Ubiquity of organisms, natural history, S. Falkow
plasmid ecology and introduction to normal (U. Washington - USA)
bowel flora ,
General problem of Salmonella infection in E. Anderson
man and domestic animals and the use and (Colindale - UK)
misuse of antibiotics in medicine and agri-
culture, enhancement of R plasmid emergence
Plasmids which contribute to virulence or. H. Williams-Smith
colonization of enteric species (Houghton Poultry
Station - UK)
10:00 Coffee Break
Gene transfer in vivo of both enteric M. Richmond
organisms. and Staphylococcus (Bristol - UK)
Panel discussion on ecology and potential S. Falkow
dissemination of E. Coli strains (Chairman)
12:00 Lunch
1:00 A Public Policy Perspective H. Green

(The George Washington

Univ. National Law Ctr.)
Monday, February 24, 1975 cont.

page 2

 

 

 

TIME SUBJECT SPEAKER
4:30 Molecular biology of bacterial plasmids R. Novick
(Pub. Health Res. Inst. ~-
USA)
Molecular biology of bacterial conjugation and R. Curtiss
conjugative mobilization of plasmid and other (U. Alabama - USA)
DNAs
Phenotypic expression of plasmids in bacteria J. Davies
(U. Wisconsin ~ USA).
Some features of DNA uptake by bacteria M. Fox
(MIT - USA)
6:00 Dinner
7230 Genetics and biochemistry of DNA restriction H. Boyer
(U. Calif., San Francisco -
USA)
Molecular cloning of DNA as a tool for the study 5%. Cohen .
of plasmid and eukaryotic biology (Stanford Univ. ~ USA)
Development of bacteriophage systems as cloning ~~ K. Murray
vehicles (U. Edinburgh - UK)
TUESDAY
FEBRUARY 25, 1975
8:30 Panel discussion on experimental guidelines R. Novick (Chairman)
for novel recombinant prokaryotes (Pub. Health. Res. Inst. -
USA)
12:00 Lunch
4:30 Plasmid-viral DNA Recombinants

 

Design, construction and value of new kinds of
molecules using SV40 (deletion mutants, trans-~
ducing viruses, plasmid-viral gene recombinants)

"Natural history of viruses"; especially, are
viral genes attached to plasmids a special case?

Adeno-SV40 hybrid DNAs as a case history; includ-
ing: a) NIH distribution policy, b) conditions
required for use in lab, c) results of risk
assessment experiments.

D. Nathans
(Johns Hopkins Univ. ~- USA)

J. Sambrook
(Cold Spring Harbor Lab-USA

A. Lewis
(NIH - USA)
Tuesday, February 25, 1975 cont.

page 3

 

 

 

 

TIME SUBJECT SPEAKER
6:00 Dinner
7:30 Comparison of SV40 and human papova viruses: D. Walker
pathogenicity, (cross reactivity and onco- (U. Wisconsin - USA)
genicity)
Infectivity and transforming capacity of SV40 D. Jackson
+ SV40 DNA; use of recombinant DNAs to infect (U. Michigan - USA)
bacteria: vs. mammalian cells
Isolation and study of Rous sarcoma proviral M. Bishop
DNA presence of "virogenes" in "normal" cells (U. Calif., San Francisco-
USA) |
Possible experiments with herpesviruses DNA B. Roizman
fragments (U. Chicago ~ USA)
Panel discussion on guidelines for virus- A. Shatkin (Chairman)
plasmid recombinants
WEDNESDAY -
FEBRUARY 26, 1975.
8:30 Plasmid~cell DNA Recombinants
What has already been done in linking cell and D. Hogness (Stanford - USA)
plasmid DNAs D. Brown (Carnegie Inst.-US
D. Carroll (Carnegie Inst.-
USA) :
10:00 Coffee Break
Major problems which can be solved by making 5S. Brenner (MRC - UK),
cell DNA-plasmid-cell DNA recombinant P. Day (Conn, Ag. Exp.
' Station - USA), R. Valen-
tine (U. Calif., San
Diego ~ USA)
12:00 Lunch
4:30 Panel discussion of guidelines for plasmid- D. Brown (Chairman)
cell DNA recombinant experiments.
6:00 Dinner
7:30 Brief analysis. of responsibility of research D. Singer (Fried, Frank,

scientists and of risk balancing. With touch on
ethical issues insofar as responsibility is con-
cerned, and focus on the serveral components
involved in balancing the risks and benefits

of creating recombinant DNA molecules

Harris, Shriver and Kampel-
man, Washington, D.C.; Hast:

ings Inst. of Society, Ethi:

and the Life Sciences-USA)
Wednesday, February 26, 1975

TIME

SUBJECT

page 4

SPEAKER

 

Legal liability of investigators and institutions

R. Dworkin —

in the event of proximate or remote injury arising (Indiana Univ. - USA)

out of work with recombinant DNAs. Applicability
of Occupational Health and Safety Act. Infotmed
consent, insurance and indemnity.

Institutionalization of current and future experi-
mental guidelines, role of NIH Advisory Committee,
international co-ordination of policies of
different nations.

A. Capron
(U. Penn. Law Sch. - USA):

 

THURSDAY
FEBRUARY 27, 1975

 

8:30

12:00

Discussion and adoption of conference statement

Ajournment