Dilot

Eco PNA, @ 3a 0/4 OIF OPS 050 975 078

DeAd * ae 4 00S 1 00S O08 POS p08 00s

St ner

7 rate Down G. cok DNA with RNA Poly merane
(also & UV aad chock on BSA iA_tneubahen)

(vee 2-19-62 LY inhcbhon. by move than fe Usual 0-076 pK
ree “0 PeA O25 mi reach

Fact ihe: 0-05 mi TAKS mvt” SO Mave inecbahn
O03 me Bim ZK
(0.0% bi phos phot mun tar fe
CONS “ Stock sor 49 O1%cpm/.014

FR Be Bleue

ng Fld pu Tpunors fil 1 O10 +00 —_ «O10 7000 “010 ye

fopemolesP{f

¥ UDPR 47-61 San@z ee (nt OF9

muwoler OVA par ardeq 0
JEn zyme hos. lost activity!

WO boast HIS 040.050 065 1090 .030%K

cpm Cie mn) {4 109-7 foo. g 7 8 0k tage
-B MK 89 16 et

: aot
S o3e

— Se enero reece: Rp ET

 

[0254 DNA about an. good Oo -O7S.. se Would ach pola hve by.
mon. RNA Han DNA Whee would have crit ~ od vanta aye:

Yi seems BD Lnthense. & Ctivily. Some habe ge ee noe

 
Protas on = Methylar
Loe

Sot
Pk

(0-014 TH my fd sand

wt
_ AP

—_

£00)

0.007 _Mathgl we
0-910 Matholare

44S
490

Yq

FIO

sage

3- 1-63

Abseeo RK my MS I
Ot osq
HA Ug 360 079 74

2741060 IR°F jd gH
Ugz 10% 10-4

uyd 333

gs» Ssl
=

 

ae re one _ = ee pe ee, see eee ~~ —_
eee nee ee een ore ate ae _ on en ae at teste _
eee om eee os seme ae nner ek mane a me ne A a we fn te rman meinen ee ne
 

 

 

 

 

 

 

3-IS- G3
Cold triphosphate miyhre. New Hot Té phosphate Ue
md mw
UTP 21-7 pw | 046 / 237
ATP 22. & pm /ot ogy & 22%
CTP 24-3 fan] -OY| / / 2¢ 4
o
GIP 24: Y pus out / . 2
172
HO .22¥ ; +6Y
.400
att . Ss
Aot cre SChuwar 6206, pe] ‘So
oe OLA
, ~ anh
4 e a °
Preineobahon: ; / / awe cobohsu. 30 @ 37 f
Tris: My pn 7 a J ONA H,0 i Pre‘ine Meth; | ese Ameue Metait Ao ve |
Iti Pecold = Mn BML Fe cokk we Ve dN See foep cena
1 |.040 .osn “010 9-03 boas .048 .2¢0 |.200 + 'I8S ors 1030 -var G00
2 1aS0 2b +200 'go bis +080 + O57 + You
3 |-oyo ,OS0 1610 +3 Orr VRE ase 1200 H1S8 Ol OS + OH
y 1Ly0 12S , 200 .iye os - -O38S -Yuv
Pddaml Sh TCA. Wash on millipom 3x € 1%. TCA.
Pot maisls pore ln Counting viot c
Tap 4 10-718: . se .
_ cpm
tobe (3 mun) ~ Bf Poly marae Agackion mixture ha; Litle or
| 950 ai no eFechk on mMmethdase rasactio.
i |000 644 Can 4° ahead vith this.
234 \
4 306 ;
Bgl a 23.6
The uaz Son gen

3-2-6232

Sup fom alco hol preccp italian
Concent: B® ( wl. Count OF mk of ysuok CE 1m I-60 NA yon)
Wr Secatiilahe. Mu (Fe) Mi (+4)

Frock rata Cmsolob & Ofter aleohok PAracrp oahu.
Qdd 0.) mh 0-00 N Tar: SAU ded not dicgolve.

 

compekl. Aad bo nt (=? to NHGOH . # 10, fi
Blead = Wee
10 MUN Coons
% 4 fo 4 Check onsef quench
cen 230-6 (Sk 0 2344 230.0 4s #i0o wag ad dod
dopl VAT GF Md2 23).3 2289 9.01 nb of HIP ey Me -
4163.9 cpm.
O-O1k aloe YOohk&
GOOMeye +
2344 so hos
Tes Se 4163-49 Qe. 44,
W230 %

Then vey &w counk let in what dant dissolve R~
SOCAOSE oder

Bop What ar Counk that We soloble ote in EH aft.
ex fens ue dialysis %

Plans concanhat down, chomah aph in prosp hak - Amalf.
Tracimen duvs amont of satt- (uth ho pe fu Climate qc oly.

Even di eu Ub & put on samele- “Discard -
He- 3-63

1Apiy Ot mh Tigash = Gbove bo Cla.

Sephadey G-100 Lot no To-33

|4O- LOO Mesh -
Bed volume Cm\ be d Per g dy qt ) iW-26
Won Agyarn Cg wala PH 4 diy get) Jor |

Ve = V6 + vi + Vg = 20
oOo Ff Vo + lO + V4
lo > Vo + Vg

a / gm cotumn is probably Olc Por O1nil afiguok.

7.f
Suspend / gm 67 O-o1 mM iris, pHs, 0.005 M NaCl

Poor. column: _
Soo ml Tus Na 4
OS mi SMNalk {= 09.
Smt / Mit PH ?e3 >

O-s(s)= 25+

[Olan wosh @ Tes: Nee
J 19 0.095 WHA

Detim Vo with DNA, 9-92-60 Los car
s\ ha by a vt 4 above boleh .
td any is

 

Wash coloma uith some Cf satk- bueSex,

Apely Sample Gs im plan -

Sin
3-1T-63

. oos [S00) 2 28

sli) = S00 Lor)

Oth wy abot.
Sephadcy G- 10°

Vo

Fraction Vo}  ComNoP 0D 240
l 1.3 [3 Zz
2 }.3 2-6 z

Ww

SS

¥\

Singer
dah Ceant) hai

Why to Ch hog ODs?
Wash Coe thee @ buLw- sad

Ir is Some thing From Ybeng ur
nver Flash:

ke poor column: Clrange Bb Lohbe.
tobung - Bev OD dawn t o.04r
with abot 200 L was.
4-4 AM OD Gbour 0} wesh Sum, dova Boys
Change fi ~-2em 00 LonG@s coftumn:
Continve washing , Chang b pPuP-e.

 

Yo Wash agqacn  Survteh ph Fz bu Sfex.
Wesh comtny outy og down te Absayor ~- 028. only,

Aeply DNA an above.

Actvoat fed Vvol= Wed3le O73" © 13,8 On

| qm Coloma, but less. 13,9 7 Vo + }O + Vg { Vo 28.0

fusr amounk bs than oe

/ SO Musk

Qast Awe UN MEPur ring :
Y- | &-b?

Cheelc achi vity of ENA POLY NUr KEE:

MO
Each tube 0-085 ml Tare mee tae

Y O-03 rk LM BME (06% 14K —> 10 |
4 0. ye fas 03
7 Ou : preserews ne Jo min. tnav bation
_O-o1 luting Flor d ‘
6.13 Olek Oe pme [mk Add orn Sorylt BSA
Bk 3S To Pen.

Wath 2% oT Bak 3.5 To PCA

( 2 3 Y
b27 t L
E-coli DNA £39 ODft — O30 1.050 ,0S0 ~ Biand= REF 10.3 cM.
DERE Ae , O10 - O10 Stand = iy esy
o1°o

ayo

49 .
1060 . 960 1650 YO

Mot O24 gg

cpm. (10 ma} log Hes Gil 33.%

E nr re quile dead:
Qin
on

Cop hady G-too
Vo Detm 4-17-63

Tobe | Vo I iD 264 |
| 00) 924

2 “1.08 | bys

S$ 0-41, OHS

¢ 0-48 . ost

Janahwm due & cons.

sf. (0 Yoo Vana hens may he dare & Lael of wathag
> OT

6 L1¢ 102 of eotle Ln lbehueca SAME ws:

7 1-4oO i€y¥ .

y hig | /.03

Gq - |-co 99S

jo fo 1¢73

i wf .6 72.
pew | bee

13 17 67°

I CSE 62

[fs yc 6s”

[6 35 Gee

Iq f@ 313

Ie 34 Az2 |
¢ 42 ey

Qo OF. hae
Vo ao Pe

 
GLY ane

To lLoak at Phos pho rolyses of da meth sen A Y- 1¥-63

Blend * 13-9

 

 

New reachon muylure - Y ~ 1% 63 Diules count 4 -1G 08 S00 M.
2 -
Hml OF Mins pHPL Plat. epr  ~Bhet pels
-ant 0.os M™ EDTA
< ‘oS - © 7
Bugs 24 6 -2-m en TA S¢0 S46 fo. Fexho ju %x 10°
0.03 me PF 27. b fem | 3/63 ach 1 Jors | 07 lee Tw
23 - Cc
2° OS s . 103 : Ve
7 Sas JO. EXO «O03 gx 322 © Gp
3,606
bf 9 Q

Each be: 0.03 me AE mey. Sf
bor 4d COS MM Mgt
BS 2 mg [ot BSA oe usual ts! assay,
0-0f rl O-1MIGHPOY
JS 4 «
powwA pety U  dernechyt Eco SANA Anibles

see SRNA NEY, 25-67 pooredSep HO COM BR mutes
Sry jot mn 32S 00/4 328 00]4 thte tp wf CLP pre F
Tam Aq i oi
Subytres
. “
Oss
1 tbo reso HOY
- .o010 oos 0300 VIO 025k S04 ON
3 O10 L035 e2.7
Yy “Db 00s = 1030 Go” YOU 'O20K 6.4
- 005"
s abe OHO Hb
1008
C 18 ‘oor ; (038 150 104 9632 0.465
7 po = 1 OL6 44.4
5 pos = O0f 08S 724 QE. O00 F 0. (3

CHa. | mt Cc pe A. Cy pect abut Gu [nk, Sea Yo 16> 63 HMw:
ye pug ued Wh had Lr as muck PM aw bee: That ( one appa

fern w . Py gts

Xv
AY oe
_ | . Y-14-63
Expt #2 on Phospharalysis of damethyfSens paw

 

lock | a exlent oe phosphine
Ee Pp bx EN 2 Y pre concenty ahn
Chaat 28 om

Re Muctou : w @ | he rmove Q. moat (ho fom, 2h.
LM P** O12 (O12 J VUSvat noatt Assay
Mache orm 0.04  o-0Y us ,
2ng fet BSA : 0-02 (002K

O1M KH POq © 00¥ O04 v

deme thyt SNA | 0.02 0.0L f

Ap- lh 63 Lobe | O12 amb

Hot od 0,64 ob d

tt Deseep' cpm

2 > phe — 3h 9
qo! S42
2 Jo! : 39.4

3
Y
Sf th bo.3-
6

+ dow not pepe fh ox pouienes o| op e yihare,
dae seen & . re Pern 202A Mor CW IO mon tier
Mert chev i! eyet © poty A aA eth

 
—)

U-22?-63

 

Ww

TA Le uo

Tne ed cernacndin- a aes rnt-

Oa

 

 

 

 

alco. et wm ta out} in sone ew. Posten & “pas be

|
comp (ately down, = 10° bath Cats dgrtrred somne what

Man problem , heated: or ot LEE B LEO°l, Ose. SRarg
cetluloce nook fuloe | / : |

i

i : i
: / t

i .
oa

XS above | Looted orples nr" a Te “e = ~~ 6° Ch.

 

bat both ©; o>. Seay “4 Ido. Bol
Clear up How | Fup. duty. ppt. | : |
ha f Sup, | Pasty forbi J « mores : : |
| i .
Wash: Bork | paspita te 7 oe me if ba biee A Gatucfugs 30!
a bove : Combure Wah & | sept : ! :
O Ie ) ! ! !
get
Profec debm ee ee ee ee
: 4p Asso cee mgt
Ph ante | | _ 506 et ! ! | | : :
SFaad, 4 my)4 | oy rn oe : 3 5% 1074 : | | 3
@ \ae 1© ! “oro” 44° ! O75 | 1» 1b i386 ee
0 "| 0d; Ed | bu OF 82 Ub | Ys | |
po" 9087 ES Pe 1s 07 33 ( |
On ore AP oS Hb gg 1 EDS. | :

as

'
;

p vy Oy Of cells and sovicalhy Po ENA pots rer crt pee:
LY gm batgh kevienkor gg cok p| aisfex} | OO | | To
Thaw | some at room Je mpera vce 7 | ! | |

Pham el | ee

In cmall amnovat add & suspend Well (av gmt by MPa

Stu 0 mh, in | ice beth -€ mo.qnefic Sr: :

ce day (ee | - alquhoR
path . Ded not dep i proke cee He! w WU Cos: Same dondiherg
Soriges ,

Y-1F> 63

G-100 Sephadiz Colomn

Wash© Oo1.N Ws, F3 Onh | Wagey = 9 044% and skady
0.C0s M Na te2

Year SRNA Can ben-Sanger 6-24-61) 4.3 my [eek Smale peryds
My Ol} mt OU ahon by ttn. Aeply t colomn. Steart coliechny,
Wash tn with 2 OF ud waster. As fust : cottget eo Nn:
surfer ad eoeryie » | | |

i to pO vack ox less.
i ‘ i

. eum Od Ta tot
VoR To8.

Machin Veh vot 260 Fact Vet Cumul 9
| OG OYe 44 eo W793)
2 fo 4 ay | | 50 | Oe FS th
3 Oe 2.75  @3f 2 i 24S ire
4 op 365 ON | tof 280 027
TO | 4.3 s [$3 Ub +3 : {.§ (27,60 O03
6 0.75 | ol 0 25. | LOY -  2Y OGY 23.58 brY
2 0.7 5.0 (als os 75 0d aye S¥ 433
g 675 6.5 5 pAb | “72 de 0-19 24.57 30
4 OV 19,35 0.46? | : |

 

16 bos P07 019
Coom®C4 aks Tap t

jo- 18

A4- 23-63
Sen

Ach it Aesaqs (ena polymuase don EF. col extracel .

 

 

Tak Pricgloxe

osm x
Y

53.4
db

722

7

| Cach bbe: ‘cos Ts mak
(0.04
O03 AME
| poe
O-o7 dilupng flud
oO -1(3 4:
/ 2
&- Cou DNA C34 00/e- - / O50
CI : fe? 1060 |
HD ,06C O10
V \ oh t wR
pen Usman) 61h 8%
cpm Sa (65:3 54
— lope 44 4I 35
vb (per bn)

meamayes [= ~ Unk

we] A

 

 

| 0! endaid~ | 21,1 00

3-IS-68 da ded ve, of TP muchitle

fecount - ¥isSo Ei PVC pm |

or peet Fers4s F390 + IEF

 

vy24
————
= ¢
ore |w 4
” the
OSO +02
020 , 095
v 6 7 r
i gol
397: Brash Pe
ar  SY44¢ 25.2 Kyso
38EF 543 (22: U (F3H .
364 giao (8356
Ib O |
(647
B 25-6,

Reassay Crv de Pokaan a se (sonicate) a fer Shwe ge in deep Fras wR Se Voy

| \ : i : |
| ! | | {o- 45, apa
!

t
t
j
i

}
Each ‘Whe 0.05 me Tats my hore 4 Bk pa perf 26-2

, O08 at Ta P amu 3-15-63 4 ON s Feed A100
0-03 nk pme, om A :

i
\

O-Ol M HY long ‘Foo. di S 18" as vsvek

i

; i2ao brie

, aan
& Cote, ONA ‘ 3g ont —_ : bh | an
Sample et “fy -2? _— 1 OSO ,OSO 1g 2s)

Uo EIR oJ 070. 097 Y
tu 4 4 ! 4

! yar |
cpm berms) 273 35> 313

7 Rhea  32e “297 .
This evhiaer cof fori ng frosen day Aas dort Aetaceé, abut

 

i
30 ‘lo of us ac hu, >. IMNISre - .
'
i :
i i i i
: | : :
: i i
: :
i L 3 ‘
: : ; :
i +
\ :
i i i ‘
j { !
: !
‘ y a 4
, } 1 .
: 1 : :
5 5 . : :
: i j ! i
i ; i :
: i
: ‘ : i i
i : : :
i i i i j
2 : 4 i
i : i i ;
i ; { ‘
: | | |
; i i j
i : i i
i bee i
: } :
j ‘ :
: : | .
a
' j i 4 t
Assay E.co& alleatne prosphatase.

Ya2s- 63

 

  

ib Ab pom clea ke

  

3 xy 2 ' ) 37°
0-01 oO-ot < |
Ines, O-S MEH EZ 0: 0S t b-esg-— 60 2°95 Prdd 0-4 ot
\ -63 Revkt D.O} Oo O- OF
S-AmP au.s w OOS 0S OS 0:94
- a DOS
Emon, ded '-100, goult a OL 00s 1029
0-07 F OTe O60
Ko - Ost
q “O28
{ 2 4 >
Vag, O-S M pl &2 -O) Ol Ol /O) 10!
\ 27(G-63 Asubrad
S- Ame Quy uf *O| *O} O81 OO; }
dtr-feo. Pm] Steud 74
Shou sample 6120A s1662 coum _ +601 -00S ee: os
deb INS forge _
Use sample e1r0A 299¢yuJ4 — - 001 00S
Ho 08 074 O18 1.09 1095
Abs $20, O14 O10 322 ~O4Y 107
pomoles Po 000 G 0038 «Os .ooly “bous
— BL (0032 1.612% + CO1\D +0034
motes fy my -6107 ‘0430 OO4O 0130
famogs [mL onrgne. 1o70 7 £ GO Yoo 260

fommoks [ho [rt enry

Z2x6 2080

 

Plante eee —stect

2.0 PCA

take 0-3 for Arse Py

~O nine te

12 00 7 Re

2A rrphe-—--a-beovk- -/C-0-8 fro

ver dahon |

102m
pe” Pi

G17

5 a
! lan bose stock makual mevees fr allcaleu dageats
(4-25-63

Te st Ap tte 2 be nuclease

 

Blank ont Nuyorro 3 2s 100th 21 Cpm

 

 

 

{ ' j : i } n A
| i brand : | #.008 eS BMA e919 epm
. A
Cold aur imu: } Plae v a, 9) 8, bale Or & wh of
2 2 wl OS M Tres #2 | ced soto he + Onl ml 1910 Ney,
: Ord « O- OF mM cér 4 10 wh: Bays Coons Tap F, 10-7
©. & aa M me Cts Otters heed 00 at 260 4a 3,6,4 10
4 " ;
6 3.0
i : ' ; ; :
WA | 3 | SeNA 00S Phgnle oOe MK P04, C-o1N Wa CL
| Abs. Yo dk: 8 |
: ‘ } COT » orn
eth | |
. Por BY Bg is : 6.7 & oF f9
S ey May | 4) 1 O30 O30 1.030 » 030 "030 (086 030 . ©0230 “026 10 30
Vv poty A Sey Taloa rPfo ‘ Oro O10
y 34.
S RvA a4 Fiaigs 1 005. 00S | OOS |
J sed yen Lo : i QB : sdk © 9 (0
(™ chga ele, s :
H.0 , 0 bo 16C |} 065 —«.OSO | 08S 05S " 959 08S oss, ous
t
Fume yh ih th Oo 7 Oe thy pho th th
pt] 25D PCA 0-9 ig. | 0-9 OG ' 04 (09 .0F a4 184 09
: >)
0 4 3 / : Hf 4 ' ‘
O0 460 | OE wl c : yy 64d
ODdc7 | OS | L/Cl me HB i © |
_—
C hn | , obs | | B43 | 32.57
: |
i |
why high | QV fur 220 hie i
& ggnrod no nuclease | hore tw sF evylead fest @ SQNA:
: : : i
‘
;
|
x
Slow in Falund rie: hme’:

 

. 4 : t
\
4-26-63
of dc mncetnegh SeNA

 

Re peat on phos phov olyses
: i ¢

Pach bbe

O-O3 Wi ky Me Y-1g-63.

: ve
C-Ol hk Cos M mye

i

Plan esual f" assay

O-O0$ ai ang | BSA : Sa - 3%, 2b ox GIA zx 19, 700

CO(M Of MK, UPOe

Y

:
i

aK demartf~ : Ap ib6 Cpe
Sng a peew Saag Wer 0 pune wf
rites Save
O10 ~ 7 O32 32
'0fo ~ (OOS 03 0— U3S YO3 0205 4
so 046 330
coe (1028 .g20 Yor | 2 |
O10 — ore +010 (YT pf 5 05S OSS
~ 005 — oi0  ,o30 43 60 - 5636.

O- 134, lact asstyg | was ov \. & FR poty coy [d

Maat of Te esse nhathy no Phosp hovotysus Youd fp- 16 le3

Bul O- [3¥ again "A fankabraing Qeckeek ‘Muth longer

La eud alk om.

 
a

AL

(ON A Polymerase, Prro ot

Y-3663

 

55.¢ qm E.col B ( enre-) oame wtoc Bote h 3-]3 -G3.

‘Take eor aboot Gam

(w ‘r-?/_ b)
bath and me chancel. “C9 ppg, :

leder's

SOmewWhat mane quid than

Botte A & make good suspension. |

My 0 &

. magnet iW. tad AQom.

Soni fier on 1c ml batches,

each: hooks. pre Ty yood..

Com hi ge Seayvall Fo Mon.

Wash ppt wth
Foo!

~~ 30 mi
Are 301
Wash « Sup. [es.

Pore D feu.

Spneg, 30,000 #, Qo 30 head. Plan oh.

bole...

Y rom EE UA -

seth: ny 6 ( Cor )

Thaw wah ard of cata Hoo

Miy into - Goo paste. Seems

cells. Slowly mix tn. 114 ok
Coa down
S+o—ro as thy was done.

30 sec. blasts, BE on

\20 set (> 20,000% ).
Morky se sup Recent: ‘loge, logics bet.

bufKA as
| rh saved.

last fire - Centon Hey

hed only absk 3’ no
henwel4

 

 

 

| Get (428 mA: adhe Kikang through glass work. Ap.s02
eo. 20660 ~ ee mg omg
#, Blank - ‘500 on SB
t SF oes gos was *S* 14a gage 7
F010 ote go Yo 348 077
Y Ap 3ol (-¢ - OL0 “490 (ee 10256 256
seen ree 19 oe ager ee
& Ap ioe. fete wa 20) jefe 18 19S 0169 Med Tee
>? J ore cg 249 19 034% ISS
‘e Ap 302 [62 mg | o, have 13447 Des0mq 2EEO “ta ve ETS —
oer
Ao wb. /E2.5 AL by. adding $F et - bo fla A een ime

 

Add (ye? BME —>oo M -

G.B¥ wh ‘lo Shap tonuy ean Sut tate C Sqpubb, 25

GEES BYS

[es )

eC 2) = i675 (01) ya’ RT
Pru

 

tS - J-63

OVA ~~ IS mine Never Stes Any strings: “Then she cold iS miun> morc.
Spin ~ 14,0004 3 VRA ist Teguk wm hile eee eke Yyetlow cup.
Me - leg. —omwme Q.rc

Add r-50t (0 Protanine sul Foti Clitly 0-1 yo 25-4) Coors- S4s cer)
SARs fem is! 1,000 « ver. Give sue To “Vothett May iro.

Smal whilk preespchel, Male ae BOF A oorN am p Me Ss o-osie
IN-7M- Wash preciprtale witty this as be Are. Combs Bae 10,000%g

GQ" Lovades:

Pot ep + in homogent wr OX, Using totol of abst 20 ot Bu CFeaAA- My),

My 2(362.
“BME Ceanh; Boye . Obtacn 20:5 ni Sv peanatanr. chee Co td AOOM |

To 20 ml é{ Sup Above, aad B- F54 wt Ac (satd ase, aur)
Soin 1S lop seeed, Geavoatt

Small yellow- while foe tan

To Sup add 5. Wm A-<. as abu.

“Lwbeuge 2s mu Take oe eptin | nt Bu kee B. My 2163
Rte uw — 70 Roy

My-i 2m |of

Mag y2 jo-Y mg]

Maz en 63 thaw pot up 8 Col wh ale pk

}

{ AL Meet & Lu frep _ 70 Boe

 

 

 
 

 

 

    

As SCL S On Pn poy yrer ALE Prep ©. g-2768
|
Caah Whe 6 GC mt Taw my % lo" @s osvW |
Coun TAP SJ (2 pe mes) fo->8, fap 7:
O03 Ak Gl M BME vy Bind cpms 25.5
O-or mM ditulng Kurd ON Stand — 2OaS 0
2 oy op Be aE
OO SV 1930 Oxo —
Ap 204 — , bas _ 1 OPO i — —_ - - ~
Ap 302] 7 — ~ O80 oko — - - -
Mey Be) 7 ~ - — 0 fo 020 - -
Mozy y - _ ~ 7 ~ ~ - » 010 4026 «020
Le / 070 »020 ope -020 .0f70  ,ObO0 tf 100 .E6O_ +08 1/00 % "4
Cae prGone B44 352 . ~£55 N° 1496 iy 42 2yyo y2.¢ aad
~ Blend - 4 3.26 626 qa4 14a | a aut 1"
~ Bla 4 | 322 ORR AO IFFO GND 2410 18
Kb (pf) 1930 3730 S642 ea FU Ga [yso0
mumotes (4 Cons) 5.78 bi (16.9 AS.@ sy RS
mo fot Ws 224 [e970 A240 AS yoo._21,79°
so | 4s BR | ee. - Be 268 | ¢0 ge
Tota unk ts 14,0 BL 2O° 3 boo 76, 400 25,400 Pty 200
a Ls AOR [Oe | | ana lett a ete nny ee ane -
_ ” xg wet, ase With OAT

 
S- 3-63
Protecns Or RIVA Pouy mAtde Pro 2

sp CO ah RR my Sipe IH
Ne
Blane OD OE a

~

Prot Stand 24myh coor -4aS 247 QT
awe ae ; < wee we ree na ee neat ee . “ ay LOY = 2) —
My i a0 80K acl

ioe W ®

OHO S00 | Sly 4U6G 10935 fe 2.¥

My dry, OPE, SIE yee ome ead

bf ere 99 2B ee TK IY

”

co ge ne ee er ee oe a ee

meee ee ee BOF ee
4.3
to 3-6, add oo et zx

o PCA _& precplete Sit 10 spin to! fake Yeo.
las tndtaa@d tn. Cep~—AtrFH: 0.6 wh C0>- Nad.

 

Use 0-5 00 mt fa Samph.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

ane ae nes mite ee ee no ~
= ete nee. ec etmcae
+
ee a ee ce a ee erent tn me cee

 
bs -63

Y -30- 63

Ap 30363

CeNA E coli ( 2oex) Gum Felerofaky.
a2 md ‘ op = Vv 380.
Totat Ob= ~ Noo tht [sme

bone e(1) = (2+v) 03 70000 = 70 pumes

3s7~ My,
w= O6+03¥ Want ae. J
O71 = O€
kK = 0.86 wt.

¥(1) = 7 (03)
‘

of. Q SENA 2it(r)e= 7 (0-3)

2Gt MeO
at
7,9

2
GS PM-

Wash (ine eo (umn) AG SoW- Xz S0- wo ra sh hr @ dichiled Ho.
#eise-ur Bioga. ser Neg li quble O-dD.

Node A SOSPENS1 On: 0-7 meg [nt 7, CO. NLS

N\udee Upp dummy 214 1 N OH UqR Wo.
3.8 wR ALsin Volowe DB pH & ST

)
Sample Vaan qed cqlntes. Total vol- 12-¢. Take & pu
jos abwt GS © w aye pesin Jolome Alla a wash. Volune
now is 3[ x. Add 0-3) 2 OTM TA pyea - PH Cheeks OR af 2 -
Diuke or >t-o0t Ao. ODago = 0-344. OOf4 = 2¢Y -¢
Joted OD s 384-4 4 31.34 * [077 Ob. 3 Popmo day. 4 F eee Lt
Asn? on 0008 0-010 Sample: Shaw C8 Row,