U,C>, ARM? MEDICAL LIBRARY Refe*fence Division NITROGEN*5 IN BIOLOGICAL RESEARCH A Preliminary List of References to 1 August 1951 Compiled by Eleanor Johnson Washington, D» C* 1 November 1951 ffiMim i®a*E®TD®a NITROGEN*5 IN BIOLOGICAL RESEARCH introduction This list of references is not to be considered comprehensive. It is entitled a "preliminary" list beoause it could serve as a basis for a more extended bibliography, although at present there are no plans for supplementing it. It was compiled, from the follow- ing sources: Nuclear Science Abstracts, v, 1-5, 1948-1951 Chemical Abstracts, 1937-1948 Journal of Biological Chemistry, Jan.-June 1951 Current List of Medical Literature, July 1950- June 1951 Index-Catalogue of the Library of the Surgeon General's Office, Unprinted Card Supplement Army Medical Library Subject Card Catalogue Siri, W. E. Isotopic tracers and nuclear radi-\ ations* N. Y., McGraw-Hill, 1949. The references were feot checked for any additional pertinent material they might cite. Since nitrogen*5 is a "tool" which can be used in many types of biological research, it has seemed that any compre- hensive list of references should be confined to some particular aspect of its use. T7ith the exception of several general review articles cited at the beginning of the list, references are arranged chronologically with the most recent years first, and arrangement within a year is alphabetical by author. Arch. 2L aU57n * 19 5; ARMLD FORCES MEUIC*. LIBRARY ^ K WASHINGTON. B- C. NITROGEffl5 IN BI03DGICAL RESEARCH Comprehensive Reviews 1* Schoenheimer, R. The dynamic state of body constituents. 81 p. Cambridge, Mass*, Harvard University Press, 1942, This book in- cludes an excellent review of the early work with biological appli- cations of "heavy nitrogen", and a well-selected bibliography. 2. Sprinson, D. B. Tracer studies on the metabolism of proteins. In: Symposium on the use of isotopes in biology and medicine. Madison, U. of Wisconsin Press, 1948. p* 182-208. 41 references. 3. Vennesland, R. Nitrogen and carbon isotopes: their application in vivo to the study of the animal organism. Advance. Biol* M* Phys., 1948, 2*5 45-116. Review article with 200 references. 4*. .7ilson, P* Vi», and Burr is, R. H* The mechanism of biological ni- trogen fixation. Bact. Rev., Bait., 1947, 11: 41-73. N^5 was used as the tracer element in these studies* 117 references. 1951 5*. Bidinost, L. E* Rate of formation of myosin in the muscle of the rat, J. Biol* Chem*, 1951, 190: 423-430* •\6. Clark, I*, and Rittenberg, D» Evidence on the metabolism of a-acetyllysine. J. Biol. Chem., 1951, 189: 529-531* 7* Clark, I*, and Rittenberg, D* The metabolic activity of the a-hydrogen atom of lysine. J. Biol. Chem., 1951, 189: 521-528. 8* Delwiohe, C. C. The assimilation of ammonium and nitrate ions by tobacco plants. J. Biol. Chem*, 1951, 189 J 167-175* 9* Fruton, J* S*, Johnston, R. B., and Fried, M* Elongation of pep- tide chains in enzyme-catalyzed transamidation reactions* J. Biol* Chem., 1951, 192* 39~53** 10. Hoberman, H. D* Measurements of rates of protein degradation and protein loss in fasting animals* J* Biol. Chem., 1951, 188: 797- 804. 11. Kozloff, L» M., Knowlton, K., Putnam, F. W., and Evans, E* A., Jr. Biochemical studies of virus reproduction. V. The origin of bac- teriophage nitrogen. J# Biol. Chem., 1951, 188: 101-116. 12* Marrian, D. H., Spicer, V* L*, Balis, M* E., and Brown, G* B. Purine incorporation"into pentose nucleotides of the rat* J* Biol. Chem*, 1951, 189: 533-541* -2- 13* Meneghini> M#, and Delwiche* C* C* The multiplication of tobacco mosaic virus in the host tobacco plant. J> Biol. Chem.t 1951, 189: 177-186* 14. Reichard, P., and Estborn, B* Utilization of desoxyribosides ift the synthesis of polynucleotides. J* Biol. Chem., 1951, 188: 839- 846. 15, Stettenj M« R, Mechanism of the conversion of ornithine into pro- line and glutamic acid in vivo* J. Biol* Chem*, 1951, 189: 499-507* 16* Yanofsky, C, and Bonner, D» II* Studies on the conversion of 3- hydroxyanthranilic acid to niacin in Neurospora* J. Biol. Chem*i 1951, 190: 211-218. 1950 17. Arnstein, H» R. V. The biosynthesis of choline methyl groups in the rat, Biochem. J., Lond., I960, 4fs xviii-xix* N*5-labeled glycine was used* Abstracted in: Nuclear Sci. Abstr*, 1950, 4: 894, 18* Arnstein, H* R* V., and Bentley, R» Isotopes in the study of chemical reactions* Nucleonics, 1950, 6: 11-27* Material on N*5 is included* 19* Bartholomew, ir,«r. V,, Nelson, L* B*, and werkman, C. E. The use of nitrogen isotope"nitrogen*5 in field studies with oats* Agron. J., 1950, 42: 100-103. Abstracted in: Nuclear Sci* Abstr*, 1950, 4: 645* 20* Bendich, A., Brown, G. B., Philips, F. S., and Thiersch, J. B.* The direct oxidation of adenine in vivo* J. Biol. Chem., 1950, 183: 267-277. Abstracted in: Nuclear Sci. Abstr*, 1950, 4: 516* 21* Bendich, A., Furst, S* S*, and Brown, G* B. On the role of 2, 6- diaminopurine in the biosynthesis of nucleic acid guanine* J. Biol, Chem*, 1950, 185; 423-433. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 775T" 22. Bendich, A., Geren, W. D., and Brown, G* B* A study of the metab- olism of 2, 4-diaminopyrimidine. J* Biol. Chem*, 1950, 185: 435- 438. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 777* 23* Benedict, J* D., Forsham, ?* H*, Roche, M., Soloway, S*, and Stetten, D*, Jr» The effect of salicylates and adrenocorticotropic hormone upon the miscible pool of uric acid in gout* J* Clin. Invest*, 1950, 29: 1104-1111. Uric acid labeled with N*5 was injected in- travenously* Abstracted in: Nuclear Sci* Abstr., 1950, 4: 894. 24. Berenbom, M., and IVhite, J* The metabolism of intravenously ad- ministered ammonium glutamate and glutamine. J* Biol. Chem., 1950, 182: 5-10. Abstracted in: Nuclear Sci* Abstr*, 1950, 4: 359. -3- 25. Bonner, D« M*, and Wasserman, E* The conversion of Ni&-containing indole to niacin by niacin-requiring strain 39401 of Neurospora. J. Biol. Chem*, 1950, 185: 69-74. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 772. 26. Brown, G. B* Biosynthesis of"nucleic acids in the mammal. Fed. Proc., Bait*, 1950, 9: 517-523* Includes general discussion of recent research using C*4 and Nl5. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 894* 27. Elliott, D. F., and Neuberger, A. The irreversibility of the deamination of threonine in the rabbit and rat. Biochem. J. Lond«, 1950, 46: 207-210. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 564, 28. Elwyn, D., and Sprinson, D. B. The relation of folic acid to the metabolism of serine. J. Biol. Chem., 1950, 184: 475-478. The utilization of N*5-L-serine and glycine was studied. Abstracted in: Nuclear Sci. Abstr*, 1950, 4: 689* 29. El*wyn, D,, and Sprinson, D* B. The role of serine and acetate in uric acid formation. J* Biol. Chem., 1950, 184: 465-474, Ab- stracted inj Nuclear Bci« Abstr., 1950, 4: 6^0T 30. Furst, S, S., Roll, P, M», and Brown, G. B* On the renewal of the purines of the aeeoxypentose and pentose nucleic acids* J. Biol. Chem., 1950, 183: 251-266* Abstracted in: Nuclear Sci. Abstr., 1950, 4, 462. 31. Geren, w"., Bendich, A,, Bodansky, 0., and Brown, G. B* The fate of urio acid in man. J. Biol. Chem., 1950, 183: 21-31. Abstracted inj Nuclear Sci. Abstr*, 1950, 4: 517. 32. Ghosh, B. P., and Burris, R. H. Utilization of nitrogenous com- pounds by plants. Soil Sci., 1950, 70: 187-203. Abstracted in: Nuclear Sci. Abstr*, 1950, 4: 898. 33. Graff, J., and Hoberman,"H* D. On the metabolism of B-alanine* J* Biol. Chem., 1950, 186: 369-372* The B-alanine was labeled with M*5. Abstracted in: Nuclear Sci* Abstr*, 1950, 4: 896* 34, Gray, C. H., and Neuberger, A, Studies in congenital porphyria* 1* Incorporation of *5tf into coproporphyrin, uroporphyrin and hippurio acid. Biochem. J,, Lond., 1950, 47: 81-87. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 837. 35. Gray, C. H., and Neuberger, A*, and Sneath, P. H. A, Studies^in congenital porphyria. 2* Incorporation of *5N in the stercobilin in the normal and in the porphyrio. Biochem. J., Lond.", 1950, 47: 87-92* Abstracted in: Nuclear Sci* Abstr., 1950, 4: 837. -4- Grinstein, M., Eamen, M. D., Wikoff, H. M., and Moore, C. V. Iso- topio studies of porphyrin and hemoglobin metabolism. I. Biosyn- thesis of coproporphyrin I and its relationship to hemoglobin metabolism. J, Biol. Chem., 1950, 182: 715-721. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 358* Grinstein, M*, Wikoff, H. M., Pimenta de Hello, R., and YJatson, C* J. Isotopic studies of porphyrin and hemoglobin metabolism. II. The biosynthesis of coproporphyrin III in experimental lead poisoning. J. Biol. Chem., 1950, 182: 723-726. Abstracted in: Nuclear Sci. Abstr., 1950* 4: 358* Hammarsten, E., and Reichard, P. Pyrimidine nucleosides as pre- cursors of pyrimidines in polynucleotides. J. Biol. Chem., 1950, 185: 105-109. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 519* Hirs, C. K. W., and Rittenberg, D. Studies on urea formation in surviving liver slices. J, Biol. Chem., 1950, 2^6: 429-445. Nl5 of the ornithine cycle was used. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 959. Hoberman, H. D., and Fruton, J, S. The metabolic fate of acetyl- dehydrotyrosine in the rat. J. Biol. Chem., 1950, 182: 127-129. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 359. Hoberman, H. D., and Graff, J. Effects of fasting of glucose in- gestion on the retention of ammonia. J. Biol. Chem., 1950, 186: 373-375. Nl5 labeled ammonia was used. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 894. Hultin, T* Incorporation in vivo of Nl5-labeled glycine into liver fractions of newly hatched chicks. Exp. Cell Research, 1950, 1: 376-381. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 895. Johnston, R. B., I.Iycek, H. J., and Fruton, J. S. Catalysis of transamidation reactions by proteolytic enzymes. J. Biol. Chem., 1950, 185; 629-641, Abstracted in: Nuclear Sci. Abstr., 1950, 4: 834. Leifer, E., Langham, IT. H*, Nye, J. F., and Mitchell, H. K. The use of isotopic nitrogen in a study of the conversion of 3-hydroxy- anthranilic acid to nicotinic acid in Neurospora. J. Biol. Chem., 1950, 184: 589-592. 8 references. London, I. M* The'conversion of hematin to bile pigment. J. Biol. Chem., 1950, 184; 373-376. Nl5-labeled hematin was used. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 598. London, I. M., Shemin, D., and Rittenberg, D. Synthesis of heme in vitro by the immature nonnucleated mammalian erythrocyte. J. Biol. Chem., 1950, 183: 749-755. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 566. -5- 47. London* I. IT,, and West, R* The formation of bile pigment in pernicious anemia* J. Biol. Chem*, 1950, 184: S59*364* Glycine labeled with N*5 was used. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 598. 48. London, I. M., West, R., Shemin, D., and Rittenberg, D. On the origin of bile pigment in normal man. J. Biol. Chem., 1950, 184: 351-358. Nl5-labeled glycine was used. Abstracted in: Nuclear Sci* Abstr., 1950, 4: 600. 49, London, I. K., 'Jest, R., Shemin, D*, and Rittenberg, D. Porphyrin formation and hemoglobin"metabolism in congenital porphyria. J. Biol. Chem., 1950, 184; 365-371. Glycine labeled with N*5 xvas used. Abstracted in: Nuclear Sci, Abstr., 1950, 4; 600. 50. Maynert, E, "7., and Van Dyke, H. B. The absence of localization of barbital in divisions of the central nervous system. J. Pharm. Exp* Ther., 1950, 98: 184-187, Abstracted in: Nuclear Sci. Abstr,, 1950, 4: 641. 51* Maynert, E. W,, and Van Dyke, H» B. The metabolic fate of pento- barbital; isotope dilution experiments with urine after the ad- ministration of labeled pentobarbital, J. Pharm. Exp. Ther., 1950, 98: 174-176. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 359. 52. Maynert, E. !.,, and Van Dyke, H* B. The metabolism of amytal labeled rath Nl5 in dogs, J. Pharm. Exp. Ther., 1950, 98": 180-183. Abstracted in: Nuclear Sci. Abstr., 1950, 4; 359. 53. Muir, H* M., and Neuberger, A. The biogenesis of porphyrins. 2. The origins of the methyne carbon atoms. Biochem* J., Lond., 1950, 47: 97-104. Abstracted in: Nuclear Sci. Abstr., 1950, 4s 834* 54* Muntz, J. A. The inability of choline to transfer a methyl group directly/ to homocysteine for methionine formation. J. Biol. Chem., 1950, 182: 489-499. Abstracted in: Nuclear Sci. ^bstr., 1950, 4: 357. 55, Radin, N. S., Rittenberg, D*, and Shemin, D. The role of acetic acid in the biosynthesis of heme. J* Biol. Chem., 1950, 184; 755- 767. Nl5 labeled glycine was used. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 690. 56. Radin, N. S., Rittenberg, D., and Shemin, D. The role of glycifte in the biosynthesis of heme. J. Biol. Chem., 1950, 184; 745-753. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 690* 57. Shemin, D. The biosynthesis of porphyrins* Sympos. Quant. Biol., Gold Spring Harbor, N. Y., 1948, 2^8 185-192. Glycine labeled with Nl5 was used. Abstracted in: Nuclear Sci* Abstr., 1950, 4; 356. -6- Shemin, D*, London, I. M., and Rittenberg, D. The relationship of serine to porphyrin synthesis* J. Biol. Chem., 1950, 183: 767-769. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 565, Shemin, D., London, I* M., and Rittenberg, D. The synthesis of protoporphyrin in vitro by red blood cells of the duck. J. Biol. Chem., 1950, 183: 757-765. Abstracted in: Nuclear Sci. ;/bstr., 1950, 4: 566. Simmonds, S. The metabolism of phenylalanine and tyrosine in mutant strains of Escherichia coli. J. Biol. Chem., 1950, 185: 755-762. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 836. Sprinson, D. B., and Rittenberg, D, The metabolic activity of the a-, B-, and >f- hydrogen atoms of L-leucine and the a-hydrogen of glycine* J. Biol. Chem*, 1950, 184: 405-416. Abstracted in: Nuclear Sci. Abstr., 1950, 4; 599. Stetten, D., Jr. The pool of miscible uric acid in normal and gouty man, studied with the aid of isotopic nitrogen. J. Mt. Sinai Hosp., N. Y., 1950, 27: 149-158. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 959. Tove, S. R., Niss, H. F., and Wilson, P. T.r. Fixation of N^5 by excised nodules of leguminous plants. J. Biol. Chem., 1950, 184; 77-82. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 598. Uber, F* M* Stable isotopes as tracers* In: Uber, F. M. Bio- physical research methods* N. Y*, Interscience, 1950. p, 561-598. A discussion of N*5 is included. Waelsch, H*, Owades, P*, Borek, E., Grossowioz, N., and Schow, M, The enzyme-catalyzed exchange of ammonia with the amide group"of glutamine and asparagine* Arch. Biochem. f N. Y., 1950, 27: 237- 239. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 726. " "Thite, A. G. C, and Parson, "7. Comparative studies on nitrogen excretion* Arch. Biochem., N. Y., 1950, 26: 205-208. Abstracted in: Nuclear Sci. Abstr., 1950, 4S 563. Wood, H. G., and Lorber, V* Carbohydrate metabolism. Annual Rev. Biochem., 1949, 2£: 299-334. Use of N*5 to study the tricarboxylic acid cycle is reported. Abstracted in: Nuclear Sci* Abstr., 1950, 4: 724. Zamecnik, P. C, and Aub, J* Growth. Annual Rev. Phvsiol., 1950, 12: 71-100. Use of N*5 in the studv of peptide and protein syn- thesis is mentioned. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 726. -7- 1949 Abrams, R., Hammersten, E*, Reichard, P., and Sperber, E* Tracer studies of nitrogen assimilation in yeast. II. J. Gen. Physiol., 1949, 32: 271-277. Abstracted in: Nuclear Sci. Abstr., 1949, 3: 486. Arvidson, H., Eliasson, N* A., Hammarsten, E., Reichard, P., von Ubisch, H., and Bergstrom, S. Orotic acid ?«s a precursor of pyrimidines in the rat. J. Biol. Chem., 1949, 179: 169-173* Orotic acid containing Nl5 was used* Abstracted in: Nuclear Sci. Abstr., 1950, 4: 104.' Benedict, J. D., Forsham, P. H., and Stetten, D. The metabolism of uric acid in the normal and gouty human studied with the aid of isotopic uric acid. J. Biol. Chem., 1949, 181: 183-193. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 61. Bloch, K. Tho synthesis of glutathione in isolated liver, J. Biol. Chem., 1949, 179: 1245-1254. DL-glutamic acid was labeled with N*5. Abstracted in: Nuclear Sci. Abstr., 1950, 4; 106, Borsock, H», Deasy, C. L*, Haagen-Smit, A* J., Keighley, G*, and Lowy, P. H* Uptake of labeled amino acids by tissue proteins in vitro. Fed. Proc, Bait;, 1949, j3: 589-596. Abstracted in: Nuclear Sci. Abstr., 1950, 48 223. Davidson, J. N. Nueleoproteins, nucleic acids, and derived sub- stances. Annual Rev. Biochem., 1949, 25.' 155-190. The use of N*5 as a tracer is mentioned. Abstracted in: Nuclear Sci. Abstr*, 1950, 4: 727. Di Carlo, F, J., Schultz, A. S., Roll, P* M*, and Brown, G. B. Isotopically labeled nucleic acid from yeast* J. Biol. Chem., 1949, 180: 329-331. Abstracted in: Nuclear Sci. Abstr., 1949, 3: 318. Getler, H., Roll, P. IT., Tinker, J. F*, and Brown, G. B. A study of the metabolism of dietary hypoxanthine and xanthine in the rat, J. Biol. Chem., 194 9, 178: 259-264. Hypoxanthine and xanthine labeled with Nl5 were used* Abstracted in: Nuclear Sci. Abstr., 1950, 4: 161. Gray, C. H., and Neuberger, A. Investigations on porphyrin forma- tion in congenital porphyria with the aid of N*5. Biochem, J,, Lond., 1949, 44: xl'C'-aclvi. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 462. Gray, C. H., Neuberger, A., and Sneath, P. H. A* Stercobilin for- mation in a case of congenital porphyria and in the normal. Biochem* J., Lond*, 1949, 45: x^xvfU Abstracted in: Nuclear Sci. i'«bstr*, 1950, 4: 601. -8- 79. Grinstein, M., Aldrich, R* A*, Hawkinson, V*, and Watson, C. J* An isotopic study of porphyrin and hemoglobin metabolism in a case of porphyria. J. Biol. Chem., 1949, 179: 983-984. Abstracted in: Nuclear Sci. Abstr., 1949, j3: 148, 80. London, I. M., Shemin, D., West, R., and Rittenberg, D. Home synthesis and red blood cell dynamics in normal humans and in sub- jects with polycythemia vera, sickle-cell anemia, and pernicious anemia. J. Biol. Chem., 1949, 179: 463-484. Glycine labeled with N*5 was used. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 101. 81. IIcKee, H* S. Review of recent work on nitrogen metabolism. New Phytologist, 1949, 48: 1-83. Use of N*5 in tracer studies is re- ported. Abstracted in: Nuclear Sci. Abstr., 1950, 4; 520. 82. Muir, H. M*, and Neuberger, A* The biogenesis of porphyrins; the distribution of *5N in the ring system. Biochem. J., Lond., 1949, 45; 163-170. Abstracted in: Nuclear Sci* Abstr., 1950, 4: 597. 83. Muir, H* I'., and Neuberger, A. Further studies of the biogenesis of porphyrins in rabbits. Biochem. J., Lond., 1949, 45: xjedv. Ab- stracted in: Nuclear Sci. Abstr., 1950, 4: 598. 84* Neuberger, A. Metabolism of proteins and amino acids. Annual Rev. Eiochem., 1949, 18: 243-266. Reports on the use of N*5 are included. Earlier volumes in the "Annual Review" series also include brief reports on this topic. 85. Reichard, P, On the nitrogen turnover in purines from polynucle- otides determined vath glycine N*5. J. Biol. Chem*, 1949, 179: 773-781. Abstracted in: Nuclear Sci. Abstr., 1949, Z: 149. 86. Reichard, P, On the turnover of purines and pyrimidines from polynucleotides in the rat determined with Nl5. Acta chem. scand., 1949, 3: 422-^32. Abstracted in: Nuclear Sci. ;/bstr., 1949, J3: 385. 87. Roll, P. II., Brown, G. B., di Carlo, F., and Schultz, A. S. The metabolism of yeast nucleic acid in the rat. J. Biol. Chem., 1949, 180: 333-340. Yeast nucleic acid labeled with Nl5 was used. Ab- stracted in: Nuclear Sci. Abstr., 1950, 4: 598. 88, Rosenblum, E. D., and Wilson, P. W* Fixation of isotopic nitrogen by Clostridium. J. Bact., Bait., 1949, 57: 413-411. Abstracted in: Nuclear Sci. Abstr., 1949, 3: 17. 89. Sprinson, D. B., and Rittenberg, D. The rate of interaction of the amino acids of the diet with the tissue proteins. J. Biol. Chem., 1949, 180: 715-726. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 601. 90. Sprinson, D. B., and Rittenberg, D. The rate of utilization of ammonia for protein synthesis. J* Biol. Chem., 1949, 180: 707-714, ijimonium citrate of high N*5 concentration was used. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 105* -9- 91. Stetten, M* R. Some aspects of the metabolism of hydroxyproline, studied -frith the aid of isotopic nitrogen. J. Biol. Chem., 1949, 181: 31-37. Abstracted in: Nuclear Sci. Abstr., 1950, 4s 62. 92. U. S. Atomic Energy Commission. Isotopes, a three-year summary of distribution rath extensive bibliography. Washington, G. P. 0*, 1949. The 100 page bibliography includes references on Nl5. 93. Wittenberg, J., and Shemin, D. The utilization of glycine for the biosynthesis of both types of pyrroles in protoporphyrin. J. Biol. Chem., 1949, 178: 47-51. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 162. 94, Wu, H,, and Rittenberg, D* Metabolism of L-aspartic acid. J* Biol* Chem*, 1949, 17^9s 847-856. L-aspartic acid was synthesized from fumaric acid ammonia containing Nl5* Abstracted in: Nuclear Sci. Abstr., 1950, 4: 103. 1948 95. Abrams, R., Hammarsten, E., and Shemin, D. Glycine as a precursor of purines in yeast. J. Biol. Chem., 1948, 173: 429-430. Nl5 was used in the study. Abstracted in: Chem. Abstr., 1948, 42: 4235. 96. Ashby, W. The span of life of the red blood cell; a resume. Blood, N. Y., 1948, Zs 486-500. Experiments determining life span by labeling corpuscles with Nl5 are discussed. Abstracted in: Nuclear Sci. Abstr., 1948, 2: 107. 97. Bendich, A., and Brown, G. B% 2, 6-Diaminopurine, a precursor of nucleic acid guanine. J. Biol. Chem., 1948, 176; 1571-1572. Ab- stracted in: Nuclear Sci. Abstr., 1950, 4: 162. 98* Bergstrand, A., Eliasson, N. A., Hammarsten, E., Norberg, B., Reichard, P., and von TJbisch, H* Experiments with Nl5 on purines from nuclei and cytoplasm of normal and regenerating liver. Sympos. Quant. Biol., Cold Spring Harbor, N. Y., 1948, 23: 22-25. Abstracted in: Nuclear Sci. Abstr*, 1950, 4: 357. 99. Brown, G» B. Studies of purine metabolism, Sympos. Quant. Biol., Cold Spring Harbor, N.'Y., 1948, IZt 43-51. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 362. 100. Brown, G. B., Roll, P* M., Plentl, 7,. A., and Cavalieri, L. F. Utilization of adenine for nucleic acid synthesis and as a precursor of guanine. J. Biol. Chem., 1948, 172: 469-484, Adenine containing excess N*5 was used. 7>bstracted in: Chem. Abstr., 1948, 42: 3785. 101. Cavalieri, L. F., Blair, V* E., and Brown, G. B. The synthesis of uric acid containing isotopic nitrogen* J. Am. Chem, Soc, 1948, 70: 1240-1242. /abstracted in; Chem. Abstr., 1948, 42: 7251. 102. Davidson, J. N«, and Reymond, W. Nucleic acids labelled -with P32 and Nl5. Biochem. J*, Lond., 1948, 42: XIV. Abstracted in: Nuclear Sci. Abstr., 1948, 2: 111, 103. Grinstein, M*, Kamen, *M. D,, and Moore, C* V* Studies on rlobin and porphyrin metabolism made with C*'** and N*5* J. Laborat. Clin. M.| 1948, 33: 14-78-1479. Abstracted in: Nuclear Sci* Abstr,, 1950, 4* 160. 104* MacVicar, R*, and Burris, R, H* Translocation studies in tomato using ammonium sulfate labeled with N15. Am, J* Bot», 1948, 35: 567-570. Abstracted in: Nuclear Sci. Abstr., 1949, 2: 199* 105* Iluir, It. I.I., and Neuberger, A, Investigations on porphyrin forma- tion in rabbits with the aid of Nl5» Biochem* J*, Lond,, 1948j 43: Ix. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 462. 106. Shemin, D*, London* I* M*, and Rittenbcrgj D* The in viti-o syn- thesis of home from glycine by the nucleated red blood cell, J* Biol. Chem., 1948, 173: 799-800. Glycine labeled with N*5 was used. Abstracted in: Nuclear Sci. Abstr., 1948, 1: 106. 107. Terry, R., Sandrock, TV. E*, Nye, R. E., Jr., and Whipple, G. H. Parenteral plasma protein maintains nitrogen equilibrium over long periods. J. Exp. I,!., 1948, J37: 547-559. Lysine labeled with N*5 was used. Abstracted in: Nuclear Sci. Abstr., 1948, 1: 107. 108. Tove, S. R., and Wilson, P. W. Isotopic studies of fixation by rhizobia in presence of hemoprotein, Proc. Soc. Exp. Biol,, N. Y., 1948, 69: 184-186. Abstracted in: Nuclear Sci. Abstr., 1950, 4: 409. 109. Vigneaud, V. du. Migration of the methyl group in the body. Proc. Am. Phil. Soc, 1948, 92: 127-135. N*5 was used in the study. Ab- stracted in: Chem. Abs¥r., 1948, 42: 8904. 110. White, A., Hoberman, H. D., and Szego, C. II. Influence of adrenal- ectomy and fasting on the incorporation of isotopic nitrogen into the tissues of mice. J. Biol. Chem., 1948, V£} 1049-1050. Ab- stracted in: Chem. Abstr., 1948, 42; 8286. 1947 111, Brown, G. B., Roll, P. M., and Cavalieri, L. F. The in vivo oxida- tion of uric acid. J. Biol. Chem., 1947, 171: 835. The studies used isotopic N. Abstracted in: Chem. 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