Doe Wo 06888 I 
mm 
r ammtAL mu&qmmm 
till *r*L)' *** *Aaf oomka» ) 
NZixtMff xsm&t§»ea snericar, imm «Aif/' 
ALLIED flUHdUfOl ASH •<» 8BOTIOS 
by Thantro Intoll, far^at# 
Sato Bac»d ATX8 U M 50 
Ooonrtptlon of 3ont*mta: ?all ImilftlitA of *Antlbodi»« 1a Slood 
Kllfht *tonth« Aftor Inoculation with Jaaoiv 
oonic toTo-Trcmtod Oholor* fAecinA," by 
Labomiofy of Aiwy Nadleol 
Oollogo, 1940. Dee He 65388 X 
Angr Nodical Gel logo f3pl4o»l domical 
ftMtaveh Boporl 
faction 3 Vunbor 13P 
Antibodies ift Blood Months After Inoculation 
with 3upor«onic *aro-Treated Cholera facetno 
Anqr Modi cal Col logo Bpldooiology Laboratory 
lot Lt (nodical) SAW. Tounoo 
in-official staff aeaber SKDO. fokoehi 
(Secret) 
ioetlo* 3 
Original Copy 
Cl notification 
43T-4 
343-sr? 
360-.1? 
BaoolYod 30 Aug 40 Doc Ho 85388 I 
fablo of Cobtout* 
ftonoral 
Chop tor X. foot material* and procedure 
A. foot material* 
I. foot procedure 
1. Agglutination reaction 
2. CoKpleamt fixation reaction 
9. foot-tube bactericidal* 
4. laniml nation tecta 
Chapter XI. feat result a and ommarle* 
A. Agglutination reaction raaulto and wmmmej 
B. Complement fixation reaction revolta and suanavy 
0. faot-tuba bacteriolysis raaulta and aonnaiy 
D. Inamaieation toot reaulta and cunaaiy 
Chanter XIX♦ Concluaion 
Seppleawnt 
Bibliography doc so sasta i 
&*aaml 
fhe offoetimnoa# of tho oho lorn mod no la reducing tho in* 
faction rato and Mortality mto of tho dieeaee alroady ha# bom 
lessen at rated by m«A8 amt later by «0LUI, %ummmn\ 
tAMLAfU&n and l£X39I3AIZi« In our country thia fact haa boon es- 
tablished through roculta cf investigation* aerfotued in 3T090 
rmfoetum by hOTM® A and In OSAKA by HOIfOlA during oho l orn epidemic*. 
fha actual of foot iron* aa of oho lera-meclne Inoculation* t 
however, haa boon omluatod in acmral different way*. W and 
mmm ham olaived tho effective sorted to bo fro* thru* to four 
nonth #, whom** ITTCOTT, H0M«3 and XOTMyUBI ham olalnod «lx nentha. 
Thu iawualty tltor disappear# in mvm to nine nontho, according 
to 11X13X3, and In one year, seconding to FAFAWAlIi* and XMU8S * 
VMMI haa warned against exceeding tho effective period mi hao 
moanneudod that Inoculation# ho repeated omiy tlx month#{ tho 
9ey«uiaa and Austrians encountered aocceae with thia not hod 
during tho world war. 
ttotheda of Manufacturing oholora vaccine aro uuaortma, but 
la rooont day# tho offoetimnoa* of tho «u:*raonic warn-treated 
oholora mod no hae suddenly cono Into proadnonco* It haa bom 
reported already that In tho Innuainotion of huaan# tho production 
of antibodies in tho blood two week# following tho second inoculation 
proved superior to tha eoatrols. sine# the continued effect a of 
1 amine aubat«aoo# omr an ortoadod ported aaong huaaaa Imauninod 
with tha aunoraonic waTo-troatod dhclora mod no m«aln unknown, 
t#at# to cemr tide yhnee h*m been ordered. 
Ohnptar X. toot aatafialo and pmcadura 
A. To*t sutortalci fha !#•! asm canal atod of mi« taken 
on 9 Sec 3© !im 34 <ntl of 40 poraoa# belonging to tho Any Nodical 
Ocllogo Laboratory who, on 31 Nor 39 worn inoculated 
with cqporooaic wam-t mated cholera madnos (ho roof tor referred 
to mm 8.S*?,) and fro* 33 out of 40 poraona inoculated with 
control oho loro, mcciaec (hereafter roformd to a# f .?*) prepared 
by the college* 
BetalXc on tho oho*# inoculation notorial* already ham 
boon doacribod. (SahU force Bulletin n~30.) 
Alac included la tho toot# mm core t«k«n oa tho aaao day 
fro* 133 pcrooaa (ho roof tor roforrod to a# A *M.) not directly oon* 
oomod with this o*port«ont. Tho finding# worn m% uaifor* at «o*e 
of tho oo pmrmmnm hod received oho loro moelno Inoculation* before 
March 1939 while other# hml no previous Inoculation#.* fhair #om 
wore tooted, bowomr, to macortaln tho general nature of imtibodloc 
In tho blood. 
fho oxaainooa worn confined to tho a# who fro* MMifti 1939 
to tho d«y thoir blood in# taken had not boon inoculated with 
cholera wool no. Tho no m worn obtained of tor 8 «e of blood taken 
under at exile condition# fro* tho Median min of each patient 
(*»pty otonoeh) on# alimt-cooguiatod in a ntoriln to«t tubs. fhi* 
mm allowed to at and for 34 hour# In a refrigerator (4*8) aftor 
which tho mtm underwent natural aeparatlos («o»» portion* worn 
roasomd by eentrlfugaliaatlcu). larbotlc mid (0*3 per cent) 
mm added and tho torn warn atorod in a refrigerator. Ttec Bo 55388 X 
B. Toot proooduro. 
1. Agglutination r«action (Widal's mac t Ion) i fho dilu- 
tion of thm toot tin with a otorllo physiological oallao solution 
was succossirsly douhlod, starting with a 5-ti»« dilution for tho 
first toot tub# Mat sading with a 2,560-tlno dilution. Thm 
hactsrtal solution consisted of tho Kltaai strain oulturod for 
30 hours at 3T°C in an agar nodlua and suspoadod at a ratio of 0*3 af 
par cc in a physio log! sal oallao solution. 
following this* 1.0 on of tho bacterial suspension was 
addod to 1.0 on of tho rospoetiro soms-dilutions ooatlonod ahoro. 
This was ohakoA and iacruhatod for two hours at 37°C. *hs rosuits 
wort ohsorrsd on tho following naming aftor it had boon standing 
or* might at rooa toaporaturo. 
3* fJoaploaont fixation reaction: a. Proliaiaary tost. 
(1) Boaolytie sort os* A goat hoaolytis torios was 
usod. four units of homoXy si a- torn* possessing a 
ho&olytic titor of 8,400 tiaos wars astployod. 
(Soo Tahls 1.) 
2 Doe Mo 55J88 1 
feet U&« immtmr 
lM!5K>Iv«iG dll tit 1 OR 
t 
300 
II 
boo 
in 
SQO 
IT 
1.600 
f 
3.J?oo 
Tl 
6,uoo 
▼II 
12,800 
mi 
35.600 
Control 
1 
12 
XXI 
Inti/ili-iifii 
0.5 
0.5 
0.5 
0.5 
0.5 
0.5 
0.5 
0.5 
o.5 
Conplewmt diluted 10 tttees 
0.5 
o.5 
0.5 
0.5 
0.5 
0.5 
0.5 
0.5 
0.5 % MMiUtiA blood cill •elution 
0.5 
0.5 
'V' 
o.5 
o.5 
o.5 
0.5 
0.5 
0.5 
0.5 
0.5 
?h/»iologic«i saline solution 
1.0 
1.0 
1.0 
1.0 
1.0 
1.0 
1.0 
1.0 
2.0 
1.5 
1.5 
37 °c 2 
aoure 
BmiIIi 
1 
X 
h 
L 
L 
L 
L 
H 
s 
8 
I 
H 
Sabi* 1. H*ooIy«isi mlae t««t 
3 Doe Vo 5538B I 
(2) Cojapl#««»t i S#ra tmkmn from aor# than 10 iam«ii 
w#rs aixod (s«parat#4 la r«frl&«mtor) sad 
allowed to stand for mom than thro# hoars at room 
taaporatur*. Th# cowpl#»#at tlt#r was a#a«ur#d 
hofors #aeh #xp#ria*nt • dilution was approxlast#- 
ly 10 tin#s. ($•• ffthl# 2.) 
4 T)oc Be 553SS I 
Coapl«K«nt 
8 X 
10 X 
12 X 
lH X 
0.15 
x?o 
0.25 
0.15 o.?o 
o.?5 
0,15 
o.?o 
0.25 
0.15 
0.20 
o.?5 
Physiologlcel miine solution 
1-35 
1.30 
1.25 
5TC0 
1.35 i.30 
1 hour 
1.25 
1-35 
1.30 
1.25 
U35 
1.30 
1.25 
5> sensitized blood cell solution 
0.5 
0.5 
3.5 
379C 
).5 0.5 
2 hours 
0.5 
0.5 
0.5 
0.5 
0.5 
0.5 
0.5 
3s stalls 
X 
1* 
L 
X * 
L 
1 
X 
FcL 
I 
K 
X 
iftbl* 2. ralae a»aturt—at t««t 
5 Doc So 58388 I 
(3) Antlgtn* The superstate v*Tt~ti*tatod antigen 
vat obtained by culturing choltra bacteria 
(SItaut atrain) la an agar aodlut for 18 hours 
at 37°Ct suspending th# bactarla la a physiological 
tallat solution at a ratio of 10 mg par ec, sub- 
jecting 80 et of the suspension to th« action of 
880 ho eucertonic warot for 80 minutes la order 
to destroy the cells, and adding 0*5 per coat 
of carbolic acid, Ooopltta hemolysis vat dlsrlsy- 
td vlth a dilution of 3 tints or tort vhaa the 
"self-retarding action” of Milt antigen vat 
measured. The practical tlttr vat tot at 16 tints• 
(Sat fables 3 and 4,} 
i Bom So 993*8 l 
Tost tubs tmaWr 
antigen dilution 
Z 
2 
n 
u 
in 
8 
IT 
lb 
T 
32 
n 
6b 
Til 
12« 
Till 
25* 
Control 
X 
ii 
Ill 
Antlgon 
0.5 
o.5 
o.5 
o.5 
0.5 
0.5 
0.5 
lb | 
diluted 10 tiaos 
o.5 
0.5 
o.5 
M 
0.5 
0.5 
0.5 
0.5 
0.5 
0.5 
I swans sortUB diluted 10 tlass 
0.5 
0.5 
0.5 
>.? 
0.5 
0.5 
0.5 
0.5 
0.5 
saline solution 
1.5 
1.0 
o.5 
37° 
C l-h 
our lae 
ub&ti on 
— > ■ ■■ ■■—* — * ■■— 
5% sons!tltod blood coll solution 
1.0 
1.0 
1.0 
1.0 
1.0 
— 
1.0 
1.0 
1.0 
1.0 
1.0 
1.0 
37°' 
our inc1 
abut ion 
losults 
E 
s 
a 
1 
t 
1 i 
kk 
& 
a 
X. 
h 
fabl« 3. lest on fixation strength of antigen and i&auae ssrna 
7 Doe Ho 5538B I 
Test tube sumbsr 
ml 
antigen dilution 
1 
2 
n 
4 
111 
8 
IV 
1£ 
f 
32 
s # 
Central 
m 
7TXJ 
Antigen 
a.5 
0*5 
0.5 
0.5 
0.5 
0*5 
Ccmphmmt dilifcsd 2D Un»» 
0.5 
0.5 
o*5 
0.5 
0.5 
0.5 
0.5 
KhgrslologieaX —line kiM1«i 
0.5 
0.5 
0.5 
0.5 
0.5 
0*5 
13 
1.0 
yfc 
l-hom 
p ineub 
alien 
% sensitised blood eoH solution 
1*0 
| 1.0 
1.0 
1.0 
1.0 
1.0 
1.0 
1*0 
37°t 
.— 
2-iiOU3 
r ineab 
ntion 
R suits 
H 
I 
L 
t 
L 
L 
B 
1 
Tabl» 4* t&st <m practical oftlgan jfalxxm 
a B00 lo 55338 X 
(4) Tool tor*i the toot sot* wore rendered inactive 
by immersion la o 58°C water both for 30 alautes. 
(a) Kola teott The dilution of tho tost sore 
with ft physiological saline solution wee 
successively doubled, starting with a 2.5- 
tlfte dilution for the first test tube and 
ending with ft 3,500-time dilution. Test 
um to lusts was 0.5 oc of each dilution to 
which was added 0.5 ec of the complement 
sad 0.5 cc of the antigen (starting with the 
second test tube), Aftor incubating for one 
hour et S7°o 1.0 ee of ft 5 per cent sensitised 
blood cell solution was Added to each of the 
test tubes which were pieced la the incubator 
for » two-hour period. These were allowed 
to stead at room temperature. The results were 
evaluated oa the following morning. 
{See Table 5.) The fixation strengths are la- 
dieated by eyubolo H (formerly +*- ), 
K (formerly -h- ), k (formerly -+~ ), end 
t (formerly — ). (Symbol f c 1 has been 
added in thle test.) 
5. Test-tube bacteriolysis (Sets*«r-Woehsbe«y method). 
The test sera were inactivated at a temperature of 58*0 
lasting for 30 Minutes . The complement wee obtained from more than 
10 marmots. This was mixed and diluted 10 times before using. The 
bacterial suspension consisted of cholera bacteria (litanl strain) 
cultured In «a agar medium (PH 7.9) for 18 hours at 37°C wad 
suspended in bouillon (PK 7,8), Thle was diluted to a point where 
the bacterial content equalled .000001 mg per ee and Immediately 
used. 
The dilution of the test sera with a physiological 
saline solution was successively doubled, starting with e dilution 
of 5 timet and ending with a dilution of 3,580 times. Bach test 
tube contained 1.0 ce of diluted sera to which 0.5 cc of the above 
bacterial suspension and 0.6 ac of the complement were added. The 
mixture was Incubated for two hours (shaken during this period) 
at 27°c and later placed In a sterile Petri dish. Agar (approximate- 
ly 50°C) was added in amount* of 15 cc each. Bacterial colonies 
were counted after culturing for 30 hours at 57*0. The bacteriolytic 
titer of the sera was taken as the highest serum dilution at which 
10 or less eholem bacterium colonise were produced. Controls were 
also observed. 
4. Immunisation tests. 
The cholera bacteria were of the Xitaal strain which 
were cultured in am agar medium (PH 7,8) for 18 houre at 3?°C and 
which displayed a minimum lethal dees of O.S mg against white 
German mice. Mice weighing from 10 to 15 g each were employed In 
the teste. 
Xntraperl tones! injections were performed on these 
nice after 0.1 co of fresh test sera and 0.4 esc of the cholera 
9 3©« Ho 553*« I 
baeterlus suspension (0*3 mg of bacteria la 0*4 oc of physiological 
•nil no solution) worn aixod 1m the hypo do redo syringe. Observations 
were continued over a three-day period. Over 0.6 mg of cholera 
bacteria was required during the pre11ainary tost« in producing 
total death. 
fable 5. Malm toot pmcodum on esoapleaent fixation reaction. 
feet tub* amky 
terus dilution 
X 
3.5 
n 
0 
• -— 
in 
10 
17 
30 
T 
40 
n 
80 
TXX 
Control 
mi 
IX 
h- " 1 ’■ 
Sis rum rolmm 
(la*.ctiT«) 
0.6 
0.5 
\j *s 
0.6 
0.6 
0.6 
. 
Antigen diluted 
16 tlB«« 
- 
0.6 
0.5 
0.6 
0.6 
0.6 
- 
- 
0.6 
0&»ple»e»t diluted 
10 tlBWC 
0.5 
0.6 
0.6 
0.6 
0.5 
0.6 
a 
0.6 
- 
Fby»lologie*l m11b« 
elution 
1.6 
1.0 
1.0 
37 
*C 1 
mm 
Uheui 
1 
r ineubetic 
m 
-r— 
St HMillMi 
bleed eell solution 
1.0 
1.0 
1.0 
1.0 
1.0 
1.0 
1.0 
1.0 
1 
1.0 
37 
°0 3 
1 
*-houa 
r Inert. 
ibatic 
»a 
— 
S 
t 
t 
** 
Chapter XI. foot *m*alts and stwaarlos. 
A. igglutiaatioii reaction moults »ad »vww»aj7 
(So* fable* 6 and 7 bo low.) 
10 .*c m 553es x 
dilution 
© 
5 
10 
m 
4& 
a© 
160 
320 
— 
«• 
WHSL~ 
U.O.V. 
positive 
0 
0 
4 
f* 
t 
16 
6 
1 
0 
0 
• 
% 
0 
0 
n.?6 
SS .59 
47.05 
17.65 
2.94 
0 
0 
* 
i 
iUf. 
IMMr 
positive 
1 
4 
11 
10 
4 
• a 
1 
0 
0 
Jl 
* 
3.03 
ia*i2 
33.33 
>0,36 
12.12 
6*o6 
3.03 
0 
0 1 
*JK. 
Suitor 
posltim 
IB 
43 
49 
23 
15 
3 
1 
0 
0 
M 
152 
[ * 
11-84 
2B,29 
32.^4 
i$,ao 
i 9.87 
1.97 
0.65 
0 
0 
Tsibl© 6, ggluCtlnat ion mmtSjsm r-zimlts 
u Boo lo 5538ft Z 
Table 7. Agglutination reaction results. 
4" 
«■ 
0m#« posit It# to the agglutination reaction at dilution* of 
40 tlaes and over totalled S!7 per coat for the tf.S.V., ZS per coat 
for the fand 13 nor eont for the A.M. At dilutions of 10 tines 
and over tbs pool tiro eases totallsd 100 per cent for tbs U.S.f, f 
84 per cent for the t.V* and 80 par esnt for tbs A.W. £.5.7. oases 
showing a negative agglutination moot ion or positivity to the 
5-tins dilution were not *»countered* 
Das case rash was produced at ths naxiraum agglutination Talus of 
160-tiss dilution, fh® severity of ths U*8.T, casss vers post tire 
to dilutions of 380 tins* and over, but in examining the drop in 
individual agglutination values after eight nonths had elapsed ths 
values vers found to he between 40 and 80 tines, the values for 
IT,?, were still lower, indicating A drop to 10 to 30 tines. 
the U.S.y. indicated the highest average individual score, 
according to the agglutination reaction results, with a figure of 
p.8, followed by *•** with 1.7 and by A.M. with 1.0, By score is 
seaat ths exponential score represented by the exponent of prime 
factor % for the absolute value of the antigen-antibody reaction 
titer possessed by the individual serun. 
id Doc 770 55330 X 
1. Oouplomoat fixation jroaotlon moult« and mmmryt 
Cooplanant fixation otrongth wax gonoral ly voofc. In «T«*y inotaaoo 
ths ooorplouoat fixation tltor «ao mxiamjn at a 30~Um dilution. 
firm eaooo (14.71 pox a oat) voro Indicated by U.S.T,, ono onto 
(3.03 por coat) by K.f. and flT# oaooo (3,t3? oor coat} by A.M. 
(Son Tabloo 8 and 9.) 
u iMB as 5538b I 
■i#ruEk billot ion 
D 
5 
10 
20 
40 
• 
JteakJBaai 
Total mniiww 
0 
BuEsber 
positive 
12 
a 
9 
5 
0 
0 
iS 
II 
> 
33.50 
23.53 
26.47 
14.71 
0 
0 
i 
M\*ab&r 
positive 
13 
U 
5 
1 
0 
0 
i 
* 
39.39 
42*42 
15.15 
3.4B 
0 
0 
• 
A*M* 
ftnber 
positive 
74 
4$ 
28 
5 
0 
© 
M 
152 
31 
48.02 
28.95 
I9*ca 
3*23 
0 
© 
Table &• Ccmphsmmt fixation reaction results Boo Vo 8538V I 
Tn'blo 3. 0oopl«M*t fixation reaction ronulto. 
2P* 
GRAPH 
I" 
Dofiaito ws»ult» shoving « dacront® in Individual oowplo- 
aont fixation 9%T*nsih9 pm not Th« nrorafo Individual 
acorn v«» 0.56 point for U.3.?*, 0.21 point for K.T. and 0.36 point 
for A.M. t th« tattor t*o *•»!»* !••« thmn on* half of TJ.S.7. 
C. font-tub* b&otorlolyoia r»«ult§ nad ausaaaiyi (So® 9»U«s 
10 and 11.) 
10 Doc Ho 55388 1 
Smtm dilution 
5 
§ 
10 
20 
40 
so 
160 
330 
610 
1260 
2560 
1 
JoUil SCOTS 
Hal casST: 
at 
- .-Mitive 
casas 
2 
X 
2 
1 
a 
2 
6 
5 
5 
4 
4 
181 
% 
? oeitivlty 
5*ea 
2..9U 
z*% 
5.aa 
5 *66 
17.55 
14*71 
34*71 
11.77 
11,77 
i 
-i 
K.V, 
Cg4@ 8S 
a 
$ 
a 
a 
6 
4 
6 
0 
4 
a 
0 
■ osltl-rlty 
St 
6.06 
15*26 
6.06 
6.06 
m.aa 
12.12 
ibjla 
0 
12.12 
6.06 
0 
A*M. 
Pasitiy® 
Casas 
22 
16 
9 
5 
17 
20 
m 
10 
19 
16 
7 
m 
i oaitirlty 
* 
/ 
XUA7 
< 
10,52 
5.92 
3,29 
—j 
n.ia 
23.I6 
7.24 
6.se 
i 
12.50 
L 
10.53 
4.61 
Table ID. Bacteriolysis .reaction result# 
15 Doe io i 
Table 11. Bacteriolysis tool results 
a$* 
3" 
an*?! 
#ere than half (53 per cant) of the oases indicated 
a bacteriolytic titer of 330 tines end over. Approximately half 
(48 per cent) of the I.f. cases Indicated a titer of SO times *md 
over, not differing to any great extent free that displayed by A.M, 
fluctuations la individual bacteriolytic titer after sight 
months were observed, but because of the Insufficient asm»at of Mire 
and the well number of eves! nod eaeoo in the Initial series a 
standard for comparison could not be oetnbll shed. It was observed, 
however, that tight heath* after Inoculation the tKS.T. retained a 
distinctly higher amount of hemolysin as compared to the control. 
The highest average Individual score was displayed by 
U.S.f. with 5.3 points, followed by with 3.8 pointe and 
A.K. with 3.9 pointe. 
B. Immunisation test results and suassary* The immunisation 
tests were performed on laboratory animals corresponding In amber 
to the 33 persons, 39 persons and 141 persons fro® who® the U.5.T., 
I.l. and A.W. test sera, respectively, were derived. 
(See Tables 13 and 13.) 
17 Doe so 5538G 1 
Dags observed 
1 
a 
5 
Total 
deaths 
6 
6 
1 
15 
i 
(Tras 34 cases) 
lieath rate (?) 
25 
18.75 
3*13 
1 
&*?• 
Deaths 
14 
5 
1 
20 
(fma 33 cases) 
>®sth pate (^) 
4B.2? 
17.24 
3.45 
68.97 
A«H* 
S3 
21 
ia 
122 
{from 152 cases) 
fceath rate (%) 
58.B7 
14,90 
12.?? 
86.52 
fable 12. laBonizatlon tost results 
xs Deo He 653S8 I 
Table 13. Immunisation loot results 
*• 
GRAPE 
21" 
The death rote of animals employed la the teete and 
Inoculated with K.V. and A.M. practically readied or exceeded oae 
half their umher on the flret day, whereas the rate for ?.5.T, 
was only 36 per cent* the total covering the three-day period 
reveal# a 47-per cent rate for U.S.V., a 69-per cent rate for IC,T. 
and an 87-per cent rate for A.H. 
Death# among the Infected animal# vers the highest on the 
first day, hut decreased on the second and third days, la that order. 
These results shov the D«S.?. serum to poosoe# the highest 
Immunisation strength followed hy the K.f. serum; the A.H. seme 
displayed extremely weak immunisation strength. 
Chapter III• Conclusion 
A. Comparative studies were made on the anti bodies contained in 
human blood eight months after Inoculations of supersonic ware- 
treated cholera vaccines and control cholera vaccines (manufactured 
by Army Medical College) were made on the ease Amy, In addition 
the antibodies contained in the bleed of persons not connected direct- 
ly with the above experiment were studied. 
19 TSoc ¥o 56388 X 
3. The sera fren persons who had besn Inoculated with 
supersonic wave-treated vaccine* were found to retain the original 
agglutinin, oowpleaent fixation substances, bacterlolysln and 
laauae substones• eight south* after the Inoculation. The amount 
retained greatly surpassed that retained in persons who had been 
Inoculated with the control cholera vaccine (aanufactured by A ray 
Medical School). 
C. The antibodies contained in the blood of persons who had 
been Inoculated eight Months previously with the control cholera 
vaccine (Manufactured by A my Medical School) gave the impression 
of being slightly superior In number to those of ordinary persons. 
The results, however, indicated no great difference between the two. 
Supplement 
An opportunity to exaaine the blood tjnse# of a land# muster of 
persons was provldod. The results of this oxa^in#tloa have boom 
added to tho Main toxt a# a supploaent. 
the standard «orm prepared bp tho Away Medical College 
%idcnic logical Section vmn dissolved la m physiological saline 
solution according to an established rule* Blood cells of the 
highest possible freshness vers oilseed to act upon this. Mood ©ell 
agglutination eat examined and the results were dstemlned el thin a 
three ~jb! nut* period, (See fables 14, IS and 18 and Supplementary 
f»tl« 1.) 
Supplementary fable 1. Blood type 
8* 
OHAPH 
8* 
Ssz 
(l) Bleed type 
30 Bee Ho 55398 Z 
A comparative study of the Individual antibody productivity 
performed concurrently with Inoculations (based on blood typos) 
of supersonic v«ve-t rented a bo lore vaccine and control cholera 
meet no revealed no particularly marked differences. (800 
supplementary Table 2 for comparative insults.) 
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1. Anay Kadleal droup, B3VKXOAK7 ICTftm (%ldamiolo£y Manual). 
3. SAKAMTOU, Tutakm, SUIKIV rar&AHC (Serologic 
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oad Xaarunology), Adraaca traatlaa. 
4. K0BAYA3KI, 3okusa, KAMWVI SHHM0 SAIKIHOAKU (Slapllflod 
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7. SHI ISA, Toahlji and OTAMA, Matas to, VAimdAKtJ XA3VK 
(Journal of Baatarlalagy), Vo. 392, 1934, 
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Saptaakar 1938. 
11. YAffAVABI, Vatari (and thm othara), VIVOS BI3BXV9*MUkVV 
iTOftlGAKTJ 2A3SIX, Yol. 32, Octokar 1938. 
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aikroorfranianan. Id. III. I Tail. 71. 1928. 
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14. yilXfS u. WW®, Zaitachr, f. Xnnuniatatator BA, 84. 1912* 
15. lahil Korea Bullatin IX-80, 
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