PROJECT REPORT 
COMMITTEE ON FOOD RESEARCH 
QUARTERMASTER FOOO AND COITFAI HER INSTITUTE 
FOR THE ARMED FORCES 
CHICAGO ILLINOIS 
XtSKAKCl AND BBVELOONENT BKAHCI 
MILITANT PLANNING DIVISION 
orrici or t** 
QUAKTI1MASTBI OBNSSAL 
C00PEIATIN6 INSTI TUTI ON 
University of Minnesota Medical School 
iOCAUTY 
Minneapolis 14, Minnesota 
01 VI SION- “ OEPAATMMT 
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Dr. Enrique Sanchez 
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’’Effect of Spices and Condiments on the Gastric Mucosa and Gastric Secretion” 
SUNMAHY 
•Histamine in beeswax as a method for production of peptic ulcer in 
laboratory animals, has been widely tested in this clinic. Rabbits are 
refractory to the production of ulcer. Since it seemed, by experience 
already reported, that spices abet the production of peptic ulcers in 
dogs when histamine in beeswax is used concomitantly, it was decided to 
try a similar method in rabbits, 
A dose of .025 grams of spice in 20 cc. of water was given to animals 
by intragastric intubation twice a day. A dose of 15 milligrams of 
histamine in beeswax was given intramuscularly to tne animals, at daily 
intervals. Mustard, cloves and paprika were tested, A series of animals 
were given spices alone, 
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Results: Paprika. 16 animals used. 3 animals were given spices 
alone, sacrificed at 7, 25 and 30 days after experiment started,■showed 
negative stomach and duodenum. No abnormal findings were evident in 
the rest of the animals, dead or sacrificed between 1 and 20 days. 
J3 \ • ■ ' - ’ 
Cloves. 20 animals used. Four were given spice only and 
these were sacrificed between 9 and 25 days. There were no abnormal 
findings on stomach or duodenum* Sixteen animals died or were sacrificed 
between 1 and 23 days. One after 3 days showed slight injection of the 
antrum. Two after 5 days showed discrete duodenal petechiae. The rest 
of them showed no evident pathology. 
Mustard. 22 animals used. Four animals given spice alone, 
showed no change up to 40 days. Six animals showed some change consist- 
ing in some petechiae at duodenum (between 2 and 4 days after begin- 
ning, of experiment). Twelve showed no change, 
Conclusion: No evident abetting ip the production of experimental 
peptic ulcer in rabbits with histamine in beeswax can be found when 
spices in the cjjses given an© used. 
5*Apr !?**?* I 121