THE COLORADO FOUNDATION FOR RESEARCH IN TUBERCULOSIS

 

 

GERALD 8. WEBB MEMORIAL BUILDING 4200 East Ninth Avenue
Denver 20, Colorado

 

Dr. Joshua Lederberg

Department of Genetics 10/9/56
University of Wisconsin 10/15/56
Madison 6, ‘lisconsin.

Dear Joshua and Esther:
Thanks for the letter and the enclosures.

I have looked over Kurahashi's MS and sent it on with a note to
Kalckar. I made no changes or suggestions other than a couple of typo's.
( I was surprised to note in your letter that you thought that the ‘renetic
background"of -3178 "is there’ore less settled than for the members...'").

I was surprised to hear that Kellenberger is working on heterogenotes,
But he is not alone for Latarget told me yesterday that Weigle is also
working on this aspect of the problem. Beyond that I know nothing of his
work, Kell@nberger's letter is not very specific and I] don't know whether
he is working with K-12 and if so with any of the Gal mutants that we have.
I hope that he gets the importance of doing reversion tests on Gal. give
never had a culture that went to Gal-Lp that “eo ther segregated Lp or
its Gal reversions segregated. I'll get together a sumrary of the segregation
studies on W2668, which I've studied fairly well ( segregation,classification
of sdgregants, and some zood reversion studies). As you remember I'm scheduled
for a talk in N,w ¥ rk at the Academy on November 19. X¥ I've been debating
just what to say there outsude of the generalities of review statetents. This
might be a place to put down some of the r/s data. I'm not sure as to how
it could be presented as an isolated paper, the most it could be would be
descrpitive, and this might be more easily handled A&B in a review of the
lamoda system. I hate to lose the data because I don't feel that it has lead
to logicel conclusions, but some of these other people will publish it in
incomplete form. From a statement of Latarget I believe that veigle almast
has a manuscript on the production of transducing lambda. I infer this from
a question that he asked about the burst size. I'm still not satisfied with
my data; mostly from the standpoint of presentability rether than from the
conclusions that I can draw. I hate to publish things hastily or before I'm
ready to. Maybe I'll have to, to keep from losing the data. Some of the
experiments that Ke'lenberger et al are considering I did two years ago.

I just got my rotator this week and can now make lysates and do lytic
exvermments. I've planned a couple of one steps this week and if they are
satisfactory I'll try to get aMS together however brief it may be.

I've not had much sucess with val mu nts in #5100. They have been — 7
few and none that turned out clear cut from Sal, or Gal. groups. there were
a couple non-trensd. but what to do with them. t have also been looking at
V3142 and its progenitors #2753 and J/2734, This has been by crosses. I'm not
sure now that this mutation is distant from the gal group. With 2754 and Gal,
I get a protos but from 2-20% Gal+. With 2724 IT get few Gal+ and many
protos. This is in agreement with ks sther's view that there is another makker
in 2733. These cultures with the other vals have given low percentages of

Gal+. I'm at astate where I would not make any observations on distinctions
between 3142 and the other Gal-.

 
THE COLORADO FOUNDATION FOR RESEARCH IN TUBERCULOSIS

 

 

 

GERALD B. WEBB MEMORIAL BUILDING 4200 East Ninth Avenue
Denver 20, Colorado

 

eo

Itve got some one he_ping me in the lab nabt of the time. Unfortunately
this means thet ' have more trouble getting paper work done, which is what
I really need most now. I'll have to hole up for a couple of days and get
this stuff summarized.

I have been thinking about the possibility of stopping in Madison on
the A¥® woy back from New York but have not as yet made definite plans. Will
you peaple be there at that time ? I met Dr. Benjamin at a party the other
evening. He is really a remarkable individual. The combination of interests
that he has is amazing. (At this stage your letter inviting me to Madison ~
came. I will try to make it and will let you know more later about my pians)

This afternoon we are having a seminar from George Klein. Isuppose
that you have #lready seen him and talked over all his latest results. His
¥¥K topic here is general- " Exptl. studies on the mech. of tumor progression".
I suess(as you experienced) that the audince will be small and sudued. batarget
was a bit disappointed I believe, because there were no questions after his
talk. i told him later that the meopie most interested vere the ones that he
had spent the day with, and they had few questions left.I found his talk
interesting but some of his conclusions were based on opinion. He was pleasant
and telvise and I had home to Lunch one day.

Helvise has gone to work ere in the medical school. In the Ob. dept.
but not as material. She is setting up a tissue culture program, whose
purpose is a bit obscure to all. An Ob who took :ed's course is in chagge
and he has not decided what to do. Host metters requiring decision around
here gravitet& to fed co that I suppose he will give the project a dir@ction.

We received our absentee ballots to day from Bareis, city clerk. ie
will vote them but I am still uncertain whether it is legal. You got any

opinions %.

As evr,