STATENS SERUMINSTITUT Nr.

TELEFON: CENTRAL 2835
TELEGRAM ADRESSE: STATSSERUM
POSTGIRO: 2817

May 26°, i952,

Dr. J. Lederberg
Department of Genetics
University of Wisconsin
Madison 6, Wisconsin
U.S.A.

Dear Dr. Lederberg:

I received your letter of May 215% and can tell
you the following:

The culture 248 ( 0 19) was received from the Institute
of Hygiene in Basel, Switzerland, in 1941. I suppose it was
isolated from a patient Hasenblhl as the strain was sent to
me designated as "Hasenbtihl". You could get clinical data
from Miss Mislap, Institute of Hygiene, Basel.

The culture contained both a motile, monophasic (phase
1 = b) culture of S.paratyphi B and a non-motile culture,
sent to you. The motile culture did not split off the second
phase, but is no more in my collection. Biochemically both
cultures were identical, both were d-tartrate-negative, not
belonging to the java-variety. I have never found a cross-
reaction between the H antigens b and i.

The biochemical behaviour of 0 19 indicates that the
culture belongs to S.paratyphi B and is a loss-variant of the
motile, monophasic culture, isolated from the same patient.
lama little doubtful whether the O form spontaneously should
split off a motile phase with antigen i.

With kind regards
yours sincerely

PK Afi

F. Kauffmann