Dear Dan,
As you know by now, I have written to Ching-Juh about our progress. I had not answered sooner because I was feeling quite tentative about the purification and did not want to predict success. As you can see from the gel photo, the mutant was a fairly small portion of the DNA. Also, the DNA was in excellent condition being quite clear of Form II and cellular DNA.
I wanted to do two successive purifications as you suggested. This left me with too little DNA to purify after partial digestion. Therefore I have set up pretty good conditions for making Form III and I will transfect all of the DNA and then select out the full length cloned pieces.
We have now gotten a super safe vector as I mentioned to Ching-Juh. Therefore
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we should be able to transfect early in the year.
I will be sure to let you know as soon as we have some clones.
Although I have started doing some culture work, I am not entirely satisfied yet. I will work on that harder soon.
Best wishes for Happy Holidays and a Happy New Year.
Thanks so much for your help and interest.
Roy